Isolation and characterization of VGF peptides in rat brain. Role of PC1/3 and PC2 in the maturation of VGF precursor

Journal of Neurochemistry

Volumn 81, Issue 3, 2002, Pages 565-574

  Trani, E ^a   Giorgi, A ^b   Canu, N ^a,c   Amadoro, G ^a,c   Rinaldi, A M ^a,c   Halban, P A ^d   Ferri, Gian Luca ^e   Possenti, R ^a,c   Schinina M E ^b   Levi, A ^a  

^a CNR   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c UNIVERSITY OF ROME TOR VERGATA   (Italy)

^d UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

^e UNIVERSITY OF CAGLIARI   (Italy)

Author keywords

Endoproteolytic processing; MALDI ToF mass spectrometry; Prohormone convertase; Rat brain; VGF peptides

Indexed keywords

ENZYME; NEUROTROPHIN; PROTEIN PRECURSOR; UNCLASSIFIED DRUG; VGF PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN; CARBOXY TERMINAL SEQUENCE; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; ENZYME ACTIVITY; GENE; HUMAN; HUMAN CELL; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NEUROSECRETORY CELL; NONHUMAN; PERIPHERAL NERVOUS SYSTEM; PRIORITY JOURNAL; PROTEIN ISOLATION; RAT; SITE DIRECTED MUTAGENESIS;

ANIMALIA; BOVINAE;

EID: 0036316623     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.2002.00842.x     Document Type: Article

References (45)

1. 7B2 is a specific intracellular binding protein of the prohormone convertase PC2
2. Serine 133-phosphorylated CREB induces transcription via a cooperative mechanism that may confer specificity to neurotrophin signals
3. 'Western blotting': Elettrophoretic tranfer of protein from SDS-PAGE to unmodified nitrocellulose and radiographic detenction with antibody and radiodinated protein A
4. Molecular cloning and characterization of the human VGF promoter region
5. Carboxipeptidase E is a regulated secretory pathways receptor: Genetic obliteration leads to endocrine disorders in CPE fat mice
6. Glutammate-induced protein phosphorilation in cerebellar granule cells: Role of protein kinase C
7. VGF a neurotrophin-inducible gene expressed in neuroendocrinological tissues
8. The VGF protein in rat adenohypophysis: Sex difference, changes during the estrous cycle and after gonadectomy
9. Carboxipeptidase E
10. Processing of pro-opiomelanocortin in GH3 cells: Inhibition by prohormone convertase 2 (PC2) antisense mRNA
11. Heterologous processing of prosomatostatin in constitutive and regulated secretory pathways
12. Heterologous processing of rat prosomatosstatin to somatostatin-14 by PC2: Requirement for secretory cell but not the secretion granule
13. High efficiency DNA-mediated transformation of primate cells
14. Targeted deletion of the vgf gene indicates that the encoded secretory peptide precursor plays a novel role in the regulation of energy balance
15. Sorting and processing of secretory proteins
16. Hippocampal plasticity involves extensive gene induction and multiple cellular mechanisms
17. Proenkephalin-processing enzymes in chromaffin granules: Model for neuropeptide biosynthesis
18. Two activation states of the prohormone convertase PC1/3 in the secretory pathway
19. Cleavage of structural proteins during the assembly of the head of bacteriophage T
20. Glutamate and GABA localization and evoked release in cerebellar cells differentiating in culture
21. Molecular cloning of a gene sequence regulated by nerve growth factor
22. Peptide V: A VGF derived neuropeptide purified from bovine posterior pituitary
23. A developmentally regulated nerve growth factor-inducible gene, VGF, is expressed in geniculocortical afferents during synaptogenesis
24. Protein measurement with the Folin phenol reagent
25. The cell biology of the prohormone convertases PC1 and PC2
26. Chromogranin B (secretogranin I) promotes sorting to the regulated secretory pathway of processing intermediates derived from a peptide hormone precursor
27. Expression, processing and secretion of the neuroendocrine VGF peptides by INS-1 cells
28. Proglucagon is processed to glucagon by prohormone convertase PC2 in alpha TC1-6 cells
29. Structures of a gene encoding VGF, a nervous system specific mRNA that is rapidly and selectively induced by nerve growth factor in PC12 cells
30. VGF: A novel role for this neuronal and neuroendocrine polypeptide in the regulation of energy balance
31. Gene expression of prohrmone convertases in the rat CNS: A comparative in situ hybridization analysis
32. 'Proline-directed arginyl cleavage' and other monobasic processing mechanisms
33. Proprotein and prohormone convertases: A family of subtilases generating diverse bioactive polypeptides
34. The family of subtilisin/kexin like pro-protein and pro-ormone convertases: Divergent or shared functions
35. Comparison of VGF and trk mRNA distributions in the developing and adult rat nervous systems
36. Development expression of VGF mRNA in the prenatal and postnatal rat
37. Peptide repertoire of human cerebrospinal fluid: Novel proteolytic fragments of neuroendocrine proteins
38. The proprotein convertases
39. Subcellular distribution of secretogranins I and II in GH3 rat tumoral prolactin (PRL) cells as revealed by electron microscopic immunocytochemistry
40. Tissue-specific processing of neuroendocrine protein VGF
41. Mammalian membrane metallopeptidases: NEP, ECE, KELL, and PEX
42. Processing of proinsulin by Furin, PC2, and PC3 in (co) transfected COS (monkey kidney) cells
43. The neuroendocrine protein 7B2 is required for peptide hormone processing in vivo and provides a novel mechanism for pituitary Cushing's disease
44. Arginine and lysine aminopeptidase activities in chromaffin granules of bovine adrenal medulla: Relevance to prohormone processing
45. Enzymatic properties of carboxyl-terminally truncated prohormone convertase 1 (PC1/SPC3) and evidence for autocatalytic conversion