Tryptophan fluorescence study of the interaction of penetratin peptides with model membranes

European Journal of Biochemistry

Volumn 269, Issue 12, 2002, Pages 2918-2926

  Christiaens, Bart ^a   Symoens, Sofie ^a   Vanderheyden, Stefan ^c   Engelborghs, Yves ^c   Joliot, Alain ^d   Prochiantz, Alain ^d   Vandekerckhove, Joël ^b   Rosseneu, Maryvonne ^a   Vanloo, Berlinda ^a  

^a GHENT UNIVERSITY   (Belgium)

^b DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^c UNIVERSITY OF LEUVEN   (Belgium)

^d ECOLE NORMALE SUPÉRIEURE   (France)

Author keywords

Circular dichroism; Homeoproteins; Lipid vesicles; Penetratin; Trp fluorescence

Indexed keywords

ACRYLAMIDE; CHOLESTEROL; IODIDE; PEPTIDE DERIVATIVE; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLSERINE; TRYPTOPHAN;

ALPHA HELIX; ARTICLE; BINDING AFFINITY; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; FLUORESCENCE; LIPID BILAYER; LIPID VESICLE; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN INTERACTION; PROTEIN STRUCTURE; WILD TYPE;

AMINO ACID SUBSTITUTION; BINDING SITES; CARRIER PROTEINS; CIRCULAR DICHROISM; FLUORESCENCE; LIPID BILAYERS; MEMBRANE LIPIDS; PEPTIDES; PROTEIN STRUCTURE, SECONDARY; TRYPTOPHAN;

INSERTION SEQUENCES;

EID: 0036314776     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.02963.x     Document Type: Article

References (42)

1. Homeodomain proteins
2. Homeodomain-DNA recognition
3. alpha-2,8-polysialic acid is the neuronal surface receptor of antennapedia homeobox peptide
4. Neurotrophic activity of the antennapedia homeodomain depends on its specific DNA-binding properties
5. The third helix of the antennapedia homeodomain translocates through biological membranes
6. Peptide nucleic acid smugglers
7. Trojan peptides: The penetratin system for intracellular delivery
8. Getting hydrophilic compounds into cells: Lessons from homeopeptides
9. Intraneuronal delivery of protein kinase C pseudosubstrate leads to growth cone collapse
10. Downregulation of Cu/Zn superoxide dismutase leads to cell death via the nitric oxide-peroxynitrite pathway
11. Cell penetrating PNA constructs regulate galanin receptor levels and modify pain transmission in vivo
12. Cell internalization of the third helix of the antennapedia homeodomain is receptor-independent
13. Conformational and associative behaviours of the third helix of antennapedia homeodomain in membrane-mimetic environments
14. The role of charge and hydrophobicity in peptide-lipid interaction: A comparative study based on tryptophan fluorescence measurements combined with the use of aqueous and hydrophobic quenchers
15. Role of peptide structure in lipid-peptide interactions: High-sensitivity differential scanning calorimetry and electron spin resonance studies of the structural properties of dimyristoylphosphatidylcholine membranes interacting with pentagastrin-related pentapeptides
16. Effect of tryptophan derivatives on the phase properties of bilayers
17. Phosphorus assay in column chromatography
18. 2-GHz frequency-domain fluorometer
19. Fluorescence study of the three tryptophan residues of the pore-forming domain of colicin A using multifrequency phase fluorometry
20. Time resolved fluorescence and phosphorescence properties of the individual tryptophan residues of barnase: Evidence for protein-protein interactions
21. Exposure of tryptophanyl residues in proteins. Quantitative determination by fluorescence quenching studies
22. Solute perturbation of protein fluorescence. The quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ion
23. Quantitative analysis of protein far UV circular dichroism spectra by neural networks
24. Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion
25. The hydrophobic moment detects periodicity in protein hydrophobicity
26. Fluorescence and the location of tryptophan residues in protein molecules
27. Exposure of tryptophanyl residues and protein dynamics
28. Tryptophan fluorescence study on the interaction of the signal peptide of the Escherichia coli outer membrane protein PhoE with model membranes
29. Physico-chemical requirements for cellular uptake of pAntp peptide Role of lipid-binding affinity
30. Identification of a signal sequence necessary for the unconventional secretion of Engrailed homeoprotein
31. Conformational and functional study of magainin 2 in model membrane environments using the new approach of systematic double-D-amino acid replacement
32. Binding of antibacterial magainin peptides to electrically neutral membranes: Thermodynamics and structure
33. Characterization of permeability and morphological perturbations induced by nisin on phosphatidylcholine membranes
34. Tryptophan rotamer distributions in amphipathic peptides at a lipid surface
35. Toward understanding tryptophan fluorescence in proteins
36. Environments of the four tryptophans in the extracellular domain of human tissue factor: Comparison of results from absorption and fluorescence difference spectra of tryptophan replacement mutants with the crystal structure of the wild-type protein
37. Intrinsic tryptophans of CRABPI as probes of structure and folding
38. Comparison of helix stability in wild-type and mutant LamB signal sequences
39. Peptide alpha-helicity in aqueous trifluoroethanol: Correlations with predicted alpha-helicity and the secondary structure of the corresponding regions of bovine growth hormone
40. The position of the cell penetrating peptide penetratin in SDS micelles determined by NMR