Cell penetrating PNA constructs regulate galanin receptor levels and modify pain transmission in vivo

Nature Biotechnology

Volumn 16, Issue 9, 1998, Pages 857-861

  Pooga, Margus ^a,c   Soomets, Ursel ^a,d   Hällbrink, Mattias ^a   Valkna, Andres ^a,d   Saar, Külliki ^a   Rezaei, Khadijeh ^a   Kahl, Ulrika ^a   Hao, Jing Xia ^b   Xu, Xiao Jun ^b   Wiesenfeld Hallin, Zsuzsanna ^b   Hökfelt, Tomas ^b   Bartfai, Tamas ^a,e   Langel, Ülo ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

^c UNIVERSITY OF TARTU   (Estonia)

^d UNIVERSITY OF TARTU   (Estonia)

^e F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

Antisense; Drug delivery; Neuropeptides

Indexed keywords

CARRIER PROTEIN; COMPLEMENTARY DNA; GALANIN; GALANIN RECEPTOR; MESSENGER RNA; PEPTIDE NUCLEIC ACID; RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DOWN REGULATION; DRUG DELIVERY SYSTEM; FEMALE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; MELANOMA CELL; NONHUMAN; PAIN; PRIORITY JOURNAL; RAT;

ANIMALIA;

EID: 0031754150     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0998-857     Document Type: Article

References (31)

1. Roush, W. 1997. Antisense aims for a renaissance. Science 276:1192-1193.
2. Bennet, C.F. 1998. Antisense oligonucleotides: is the glass half full or half empty? Biochem. Pharmacol. 55:9-19.
3. Troy, CM., Derossi, D., Prochiantz, A., Greene, L.A., and Shelanski, M.L. 1996. Downregulation of Cu/Zn Superoxide dismutase leads to cell death via the nitric oxide-peroxynitrite pathway. J. Neurosci. 16:253-261.
4. Nielsen, P.E., Egholm, M., Berg, R.H., and Buchardt, O. 1991. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science 254:1497-1500.
5. Demidov, V.V., Potaman, V.N., Frank-Kamenetskii, M.D., Egholm, M., Buchard, O., Sönnichsen, S.H., and Nielsen, P.E. 1994. Stability of peptide nucleic acids in human serum and cellular extracts. Biochem. Pharmacol. 48:1310-1313.
6. Knudsen, H. and Nielsen, P. 1996. Antisense properties of duplex-and triplex-forming PNAs. Nucleic Acids Res. 24:494-500.
7. Peffer, N.J., Hanvey, J.C., Bisi, J.E., Thomson, S.A., Hassman, C.F., Noble, S.A. et al. 1993. Strand-invasion of duplex DNA by peptide nucleic acid oligomers. Proc. Natl. Acad. Sci. USA 90:10648-10652.
8. Hanvey, J.C., Peffer, N.J., Bisi, J.E., Thomson, S.A., Cadilla, R., Josey, J.A. et al. 1992. Antisense and antigene properties of peptide nucleic acids. Science 258:1481-1485.
9. Bonham, M.A., Brown, S., Boyd, A.L., Brown, P.M., Bruckenstein, D.A. Hanvey, J.C. et al. 1995. An assessment of the antisense properties of RNase H-competent and steric-blocking oligomers. Nucleic Acids Res. 23:1197-1203.
10. Good, L. and Nielsen, P.E. 1998. Antisense inhibition of gene expression in bacteria by PNA targeted to mRNA. Nat. Biotechnol. 16:355-358.
11. Pooga, M., Hällbrink, M., Zorko, M., and Langet, Ü. 1998. Cell penetration of transportan. FASEB J. 12:67-77.
12. Derossi, D., Calvet, S., Trembleau, A., Brunissen, A., Chassaing, G., and Prochiantz, A. 1996. Cell internalization of the third helix of the Antennapedia homeodomain is receptor-independent. J. Biol. Chem. 271:18188-18193.
13. Allinquant, B., Hantraye, P., Mailleux, P., Moya, K., Bouillot, C., and Prochiantz, A. 1994. Downregulation of amyloid precursor protein inhibits neurite outgrowth in vitro. J. Cell Biol. 128:919-927.
14. Tatemoto, K., Rökaeus, A., Jörnvall, H., McDonald, T.J., and Mutt, V. 1983. Galanin - a novel biologically active peptide from porcine intestine. FEBS Lett. 164:124-128.
15. Bartfai, T. 1995. Galanin: a neuropeptide with important central nervous system actions, pp.563-571, in Psychopharmacology: the fourth generation of progress. Bloom, F.E. and Kupfer, D.J. (eds.). Raven Press, New York.
16. Habert-Ortoli, E., Amlranoff, B., Loquet, I., Laburthe, M., and Mayaux, J.F. 1994. Molecular cloning of a functional human galanin receptor. Proc. Natl. Acad. Sci. USA 91:9780-9783.
17. Burgevin, M.-C., Loquet, I., Quarteronet, D., and Habert-Ortoll, E. 1995. Cloning, pharmacological characterization, and anatomic distribution of a rat cDNA encoding for a galanin receptor. J. Mol. Neurosci. 6:1-8.
18. Bartfai, T., Langel, Ü., Bedecs, K., Andell, S., Land, T., Gregersen, S. et al. 1993. Novel galanin receptor ligand M 40 distinguishes betweeen putative galanin receptor subtypes. Proc. Natl. Acad. Sci. USA 90:11287-11291.
19. Wynick, D., Smith, D.M., Ghatei, M., Akinsanya, K., Bhogal, R., Purkiss, P. et al. 1993. Characterization of a high-affinity galanin receptor in the rat anterior pituitary - absence of biological effect and reduced membrane binding of the antagonist M15 differentiate it from the brain/gut receptor. Proc. Natl. Acad. Sci. USA 90:4231-4235.
20. Howard, A., Tan, C., Shiao, L., Palyha, O., McKee, K., Weinberg, D. et al. Molecular cloning and characterization of a new receptor for galanin. FEBS Lett. 405:285-290.
21. Wang, S., He, C., Hashemi, T., and Bayne, M. 1997. Cloning and expressional characterisation of a novel galanin receptor. J. Biol. Chem. 272:31949-31952.
22. Derossi, D., Joliot, A.H., Chassaing, G., and Prochiantz, A. 1994. The third helix of the Antennapedia homeodomain translocates through biological membranes. J. Biol. Chem. 269:10444-10450.
23. Wiesenfeld-Hallin, Z., Villar, M.J., and Hökfelt, T. 1989. The effects of intrathecal galanin and C-fiber stimulation on the flexor reflex in the rat. Brain Res. 486:205-213.
24. Langel, Ü., Land, T., and Bartfai, T. 1992. Design of chimeric peptide ligands to galanin receptors and substance P receptors. Int. J. Pept. Protein Res. 39:516-522.
25. Egholm, M., Buchardt, O., Nielsen, P.E., and Berg, R.H. 1992. Peptide nucleic acids (PNA): Oligonucleotide analogues with an achiral peptide backbone. Journal of the American Chemical Society 114:1895-1897.
26. Peterson, G.L. 1977. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83:346-356.
27. Harlow, E. and Lane, D. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
28. Storkson, R.V., Kjorsvik, A., Tjolsen, A., and Hole, K. 1996. Lumbar catheterization of the spinal subarachnoid space in the rat. J. Neurosci. Methods 65:167-172.
29. Wiesenfeld-Hallin, Z., Xu, X.J., Langel, Ü., Bedecs, K., Hökfelt, T., and Bartfai, T. 1992. Galanin-mediated control of pain: enhanced role after nerve injury. Proc. Natl. Acad. Sci. USA 89:3334-3337.
30. Young III, W.S. and Kuhar, M.J. 1988. A new method for receptor autoradiography: [3H]opioid receptors in rat brain. Brain Res. 179:255-270.
31. 125I-galanin binding sites in the rat central nervous system. J. Chem. Neuronatal. 1:213-233.