Routine immunohistochemical staining today: Choices to make, challenges to take

Journal of Histotechnology

Volumn 25, Issue 1, 2002, Pages 45-54

  Van Hecke, Donald ^a  

^a AZ St Lucas   (Belgium)

Author keywords

Epitope retrieval; Fixation; Histology; Specimen processing

Indexed keywords

ACCURACY; ANTIGEN DETECTION; ARTICLE; EUROPE; HISTOLOGY; IMMUNOHISTOCHEMISTRY; LABORATORY AUTOMATION; PATHOLOGICAL ANATOMY; PROBLEM SOLVING; STANDARDIZATION; TECHNOLOGY; TISSUE FIXATION;

EID: 0036310373     PISSN: 01478885     EISSN: None     Source Type: Journal    
DOI: 10.1179/his.2002.25.1.45     Document Type: Article

References (32)

1. Pre-primary antibody factors that affect immunohistochemical Staining
2. A Holistic approach to antigen retrieval
3. Antigen resoration - The 'Hot' revolution in immunohistochemistry
4. The effects of tissue processing variables other than fixation on histochemical staining and immunohistochemical detection of antigens
5. Immunohistochemical analysis of decalcified paraffin-embedded human bone marrow biopsies with emphasis on MHC class I and CD 34 expression
6. Assessment of antigen damage in immunohistochemistry. The vimentin internal control
7. Conditional epitopes: Is your antibody always specific?
8. Antigen Retrieval in immunohistochemistry: Past, present and future
9. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections
10. Antigen unmasking in formalin-fixed routinely processed paraffin wax-embedded sections by pressure cooking: A comparison with microwave oven heating and traditional methods
11. Antigen retrieval immunohistochemistry under influence of pH using monoclonal antibodies
12. Effect of fixation time and microwave oven heating time on retrieval of the Ki-67 antigen from paraffin-embedded tissue
13. Study of interlaboratory reliability and reproducibility of estrogen and progesterone receptor assays in Europe. Documentation of poor reliability and identification of insufficient microwave retrieval time as a major contributory element of unreliable assays
14. Development of an optimal protocol for antigen retrieval: A 'test battery' approach exemplified with reference to staining of retinoblasoma protein (pRB) in formalin-fixed paraffin sections
15. Optimizing immunohistochemistry: Antigen retrieval and signal amplification
16. Immunoglobulin light chain staining in paraffin embedded tissue using a heat mediated epitope retrieval method
17. Microwave-based antigenic unmasking
18. Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?
19. Modified antigen retrieval procedure: Calibration technique for microwave ovens
20. Suppression of endogenous avidin binding activity in tissues and its relevance to biotin-avidin detection systems
21. Retrieved endogenous biotin: A novel marker and potential pitfall in diagnostic immunohistochemistry
22. Non-Biotin Amplification (NBA) Kit prevents nonspecific background staining of endogenous biotin induced by heat induced epitope retrieval (HIER) procedure
23. Enhanced polymer detection systems for immunohistochemistry
24. Water soluble polymer conjugates for enzyme imunoassays
25. The use of enhanced polymer one-step staining reagents for immunoenzyme double-labeling
26. Sequential immunohistochemical double-staining using envision polymer technology
27. Sensitivity and detection efficiency of the peroxidase-antiperoxidase (PAP), avidin-biotin peroxidase complex (ABC) and peroxidase labelled avidin biotin (LAB) method
28. The use of avidin-biotin interaction in immunoenzymatic techniques
29. Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabelled antibody (PAP) procedures