Lagging strand synthesis in coordinated DNA synthesis by bacteriophage T7 replication proteins

Journal of Molecular Biology

Volumn 316, Issue 1, 2002, Pages 19-34

  Lee, Joonsoo ^a   Chastain II, Paul D ^b   Griffith, Jack D ^b   Richardson, Charles C ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

DNA polymerase; Helicase; Okazaki fragment; Primase; Replication; Replisome

Indexed keywords

BACTERIOPHAGE DNA; DNA; DNA BINDING PROTEIN; DNA FRAGMENT; DNA POLYMERASE; DNA PRIMASE; DOUBLE STRANDED DNA; HELICASE; NUCLEOTIDE; PRIMER RNA; PROTEIN; SINGLE STRANDED DNA;

ARTICLE; BACTERIOPHAGE; DNA DETERMINATION; DNA REPLICATION; DNA STRAND; DNA STRUCTURE; DNA SYNTHESIS; DNA TEMPLATE; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN ANALYSIS; RNA SYNTHESIS;

EID: 0036303514     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5325     Document Type: Article

References (42)

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2. Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution
3. Escherichia coli thioredoxin stabilizes complexes of bacteriophage T7 DNA polymerase and primed templates
4. Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7
5. Bacteriophage T7 helicase/primase proteins form rings around single-stranded DNA that suggest a general structure for hexameric helicases
6. A 7-kDa region of the bacteriophage T7 gene 4 protein is required for primase but not for helicase activity
7. Requirements for primer synthesis by bacteriophage T7 63-kDa gene 4 protein. Roles of template sequence and T7 56-kDa gene 4 protein
8. The linker region between the helicase and primase domains of the bacteriophage T7 gene 4 protein is critical for hexamer formation
9. An N-terminal fragment of the gene 4 helicase/primase of bacteriophage T7 retains primase activity in the absence of helicase activity
10. Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7
11. Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides
12. DNA-dependent nucleoside 5′_ 4 triphosphatase activity of the gene 4 protein of bacteriophage T7
13. The gene 4 protein of bacteriophage T7. Characterization of helicase activity
14. The acidic carboxyl terminus of the bacteriophage T7 gene 4 helicase/primase interacts with T7 DNA polymerase
15. Role of bacteriophage T7 DNA primase in the initiation of DNA strand synthesis
16. Characterization of the ribonucleic acid primers and the deoxyribonucleic acid product synthesized by the DNA polymerase and gene 4 protein of bacteriophage T7
17. Interactions of the DNA polymerase and gene 4 protein of bacteriophage T7. Protein-protein and protein-DNA interactions involved in RNA-primed DNA synthesis
18. Structure of the gene 2.5 protein, a single-stranded DNA binding protein encoded by bacteriophage T7
19. Purification and characterization of the bacteriophage T7 gene 2.5 protein. A single-stranded DNA-binding protein
20. Interactions of gene 2.5 protein and DNA polymerase of bacteriophage T7
21. Acidic carboxyl-terminal domain of gene 2.5 protein of bacteriophage T7 is essential for protein-protein interactions
22. Coordinated leading and lagging strand DNA synthesis on a minicircular template
23. Reconstitution of the T4 bacteriophage DNA replication apparatus from purified components
24. A preformed, topologically stable replication fork. Characterization of leading strand DNA synthesis catalyzed by T7 DNA polymerase and T7 gene 4 protein
25. Purification of the 56-kDa component of the bacteriophage T7 primase/helicase and characterization of its nucleoside 5′-triphosphatase activity
26. Characterization of the helicase and primase activities of the 63-kDa component of the bacteriophage T7 gene 4 protein
27. Template recognition sequence for RNA primer synthesis by gene 4 protein of bacteriophage T7
28. Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. I. Multiple effectors act to modulate Okazaki fragment size
29. Coordinated leading-and lagging-strand synthesis at the Escherichia coli DNA replication fork. V. Primase action regulates the cycle of Okazaki fragment synthesis
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31. Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. II. Frequency of primer synthesis and efficiency of primer utilization control Okazaki fragment size
32. Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. III. A polymerase-primase interaction governs primer size
33. Analysis of the Okazaki fragment distributions along single long DNAs replicated by the bacteriophage T4 proteins
34. Gene 4 DNA primase of bacteriophage T7 mediates the annealing and extension of ribo-oligonucleotides at primase recognition sites
35. Interaction of ribonucleoside triphosphates with the gene 4 primase of bacteriophage T7
36. Formation of a DNA loop at the replication fork generated by bacteriophage T7 replication proteins
37. Initiation sites for discontinuous DNA synthesis of bacteriophage T7
38. The dTTPase mechanism of T7 DNA helicase resembles the binding change mechanism of the F1-ATPase
39. Roles of the helicase and primase domain of the gene 4 protein of bacteriophage T7 in accessing the primase recognition site
40. The nucleotide binding site of the helicase/primase of bacteriophage T7. Interaction of mutant and wild-type proteins
41. Electron microscope visualization of chromatin and other DNA-protein complexes