Reaction mechanism of GTP cyclohydrolase I: Single turnover experiments using a kinetically competent reaction intermediate

Journal of Molecular Biology

Volumn 316, Issue 3, 2002, Pages 829-837

  Schramek, Nicholas ^a   Bracher, Andreas ^b   Fischer, Markus ^a   Auerbach, Günter ^c   Nar, Herbert ^d   Huber, Robert ^e   Bacher, Adelbert ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (France)

^c Antisense Pharma GmbH   (Germany)

^d BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

^e MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

Dihydroneopterin triphosphate; GTP; GTP cyclohydrolase I; Pteridine; Tetrahydrobiopterin biosynthesis

Indexed keywords

2 AMINO 5 FORMYLAMINO 6 RIBOSYLAMINO 4(3H) PYRIMIDINONE; CARBOHYDRATE; DIHYDRONEOPTERIN 3' TRIPHOSPHATE; FORMAMIDE; FORMIC ACID; GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE I; IMIDAZOLE DERIVATIVE; MUTANT PROTEIN; PHOSPHATE; PYRIMIDINONE DERIVATIVE; SCHIFF BASE; TETRAHYDROBIOPTERIN; TETRAHYDROFOLIC ACID; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; ENZYME ASSAY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME METABOLISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; PHOTODEGRADATION; PHOTOMETRY; PRIORITY JOURNAL; SPECTROSCOPY; STEADY STATE; WILD TYPE;

EID: 0036300682     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5339     Document Type: Article

References (13)

1. The biosynthesis of folic acid. VIII. Purification and properties of the enzyme that catalyzes the production of formate from carbon atom 8 of guanosine triphosphate
2. A chemically prepared formamidopyrimidine derivative of guanosine triphosphate as a possible intermediate in pteridine biosynthesis
3. Biosynthesis of folic acid, riboflavin, thiamine, and pantothenic acid
4. Macrophage oxidation of L-arginine to nitric oxide, nitrite, and nitrate. Tetrahydrobiopterin is required as a cofactor
5. Biosynthesis and metabolism of tetrahydrobiopterin and molybdopterin
6. Homology cloning of GTP-cyclohydrolase I from various unrelated eukaryotes by reverse-transcription polymerase chain reaction using a general set of degenerate primers
7. Active site topology and reaction mechanism of GTP cyclohydrolase I
8. Zinc plays a key role in GTP cyclohydrolase I
9. Ring opening is not rate-limiting in the GTP cyclohydrolase I reaction
10. Histidine 179 mutants of GTP cyclohydrolase I catalyze the formation of 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone triphosphate
11. Biosynthesis of pteridines. Stopped-flow kinetic analysis of GTP cyclohydrolase I
12. Partition analysis and concept of net rate constants as tools in enzyme kinetics
13. Biosynthesis of pteridines. NMR studies on the reaction mechanisms of GTP cyclohydrolase I, pyruvoyltetrahydropterin synthase, and sepiapterin reductase