Enzyme activity analysis of soluble proteins using non-denaturing two-dimensional electrophoresis

Bunseki Kagaku

Volumn 51, Issue 6, 2002, Pages 367-371

  Shimazaki, Youji ^a   Hiraka, Yoko ^a   Ohnishi, Hiromi ^a   Manabe, Takashi ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Enzyme; MALDI TOF MS; Non denaturing; Soluble protein; Two dimensional electrophoresis

Indexed keywords

CHOLINESTERASE INHIBITOR; ESTERASE; OXIDOREDUCTASE; PROTEIN; SUPEROXIDE DISMUTASE; TACRINE; TRANSFERASE;

ARTICLE; CHROMATOPHORE; DENATURATION; ELECTROPHORESIS; ENZYME ACTIVITY; LASER; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; SEPARATION TECHNIQUE; SOLUBILITY;

EID: 0036299445     PISSN: 05251931     EISSN: None     Source Type: Journal    
DOI: 10.2116/bunsekikagaku.51.367     Document Type: Article

References (12)

1. N. G. Anderson, A. Matheson, N. L. Anderson: Proteomics, 1, 3 (2001).
2. M. Kinter, N. E. Sherman: "Protein sequencing and identification using tandem mass spectrometry", p. 117 (2000), (J. Wiley & Sons, New York).
3. J.-C. Sanchez, D. Chiappe, V. Converset, C. Hoogland, P.-A. Binz, S. Paesano, R. D. Appel, S. Wang, M. A. Cawthorne, D. F. Hochstrasser: Proteomics, 1, 136 (2001).
4. T. Manabe, E. Hayama, T. Okuyama: Clin. Chem., 28, 824 (1982).
5. Y. Shimazaki, M. Muro, T. Manabe: Clin. Chim. Acta, 302, 221 (2000).
6. Y. Shimazaki, S. Ohara, T. Manabe: J. Biochem. Biophys. Methods, 37, 1 (1998).
7. G. M. Rothe: "Electrophoresis of enzymes", p. 181 (1994) (Springer Verlag, Berlin).
8. P. Davidsson, L. Paulson, C. Hesse, K. Blennow, C. L. Nilsson: Proteomics, 1, 444 (2001).
9. C. Beauchamp, I. Fridovich: Anal. Biochem., 44, 276 (1971).
10. F. Sakiyama, T. Masaki: Methods in Enzymology, 244, 126 (1994).
11. B. Brune, E. G. Lapetina: J. Biol. Chem., 264, 8455 (1989).
12. S. E. Mack, F. B. ST. C. Palmer: Biochem. Cell Biol., 66, 199 (1988).