Selection of an effective enzyme for digestion of non-denaturing proteins using microscale two-dimensional electrophoresis

Clinica Chimica Acta

Volumn 302, Issue 1-2, 2000, Pages 221-224

  Shimazaki, Y ^a   Muro, M ^a   Manabe, T ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Achromobacter protease I; Complement; Fragmentation; Staphylococcus aureus V8 protease; Trypsin

Indexed keywords

ALBUMIN; ALPHA 2 MACROGLOBULIN; COMPLEMENT COMPONENT C3B; IMMUNOGLOBULIN G; PLASMA PROTEIN; PROTEINASE; TRYPSIN;

ACHROMOBACTER; AMINO ACID SEQUENCE; FRACTIONATION; GEL ELECTROPHORESIS; LETTER; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; SEQUENCE ANALYSIS; STAPHYLOCOCCUS AUREUS; TWO DIMENSIONAL ELECTROPHORESIS;

BLOOD PROTEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENDOPEPTIDASES; HUMANS; SERINE ENDOPEPTIDASES; TRYPSIN;

EID: 0033763843     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-8981(00)00329-6     Document Type: Letter

References (11)

1. Microscale multisample two-dimensional electrophoresis of proteins in human serum, cerebrospinal fluid and urine
2. Analysis of human haptoglobin-hemoglobin complexes by micro two-dimensional electrophoresis
3. Identification map of human plasma proteins: Micro two-dimensional electrophoresis followed by multiple immunoreplica technique
4. 2-macroglobulion
5. Isolation of a fragment (C3a) of the third component of human complement containing anaphylatoxin and chemotactic activity and description of an anaphylatoxin inactivator of human serum
6. Studies on a new proteolytic enzyme from Achromobacter lyticus M497-1. II Specificity and inhibition studies of Achromobacter protease I
7. Protease from Staphyloccus aureus
8. Cleavage at glutamic acid with Staphylococcal protease
9. A nondenaturing protein map of human plasma proteins correlated with a denaturing polypeptide map combining techniques of micro two-dimensional gel electrophoresis
10. Detection and identification of constitutive polypeptides of the third component of complement using microscale two-dimensional electrophoresis-correlated with the separation by non-denaturing microscale two-dimensional electrophoresis
11. Studies on human plasma alpha 2-macroglobulin-enzyme interactions. Evidence for proteolytic modification of the subunit chain structure