In vivo dissection of the Tat translocation pathway in Escherichia coli

Journal of Molecular Biology

Volumn 317, Issue 3, 2002, Pages 327-335

  Ize, Bérengère ^a   Gérard, Fabien ^a   Zhang, Ming ^a,c   Chanal, Angélique ^a   Voulhoux, Romé ^a   Palmer, Tracy ^b   Filloux, Alain ^a   Wu, Long Fei ^a  

^a CNRS   (France)

^b JOHN INNES CENTRE   (United Kingdom)

^c INSTITUTE OF PLASMA PHYSICS   (China)

Author keywords

Colicin V; GFP; In vivo probes; Tat component; Twin arginine

Indexed keywords

ARGININE; COLICIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LYSINE; MUTANT PROTEIN; SIGNAL PEPTIDE; TRANSACTIVATOR PROTEIN;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GROWTH; BACTERICIDAL ACTIVITY; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; GENE DELETION; GROWTH INHIBITION; MEDIATOR; MOLECULAR PROBE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN MOTIF; PROTEIN TRANSPORT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0036296099     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2002.5431     Document Type: Article

References (44)

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3. Overlapping functions of components of a bacterial Sec-independent protein export pathway
4. TatD is a cytoplasmic protein with DNase activity. No requirement for TatD family proteins in sec-independent protein export
5. An essential component of a novel bacterial protein export system with homologues in plastids and mitochondria
6. Potential receptor function of three homologous components, TatA, TatB and TatE, of the twin arginine signal sequence-dependent metalloenzyme translocation pathway in Escherichia coli
7. Sec-independent protein translocation in Escherichia coli: A distinct and pivotal role for the TatB protein
8. Protein translocation into and across the bacterial plasma membrane and the plant thylakoid membrane
9. Old and new pathways of protein export in chloroplasts and bacteria
10. Protein import and routing systems of chloroplasts
11. A signal peptide that directs non-Sec transport in bacteria also directs efficient and exclusive transport on the thylakoid ΔpH pathway
12. Targeting signals for a bacterial Sec-independent export system direct plant thylakoid import by the delta pH pathway
13. Bacterial proteins carrying twin-R signal peptides are specifically targeted by the delta pH-dependent transport machinery of the thylakoid membrane system
14. Pathway specificity for a delta pH-dependent precursor thylakoid lumen protein is governed by a "Sec-avoidance" motif in the transfer peptide and a "Sec-incompatible" mature protein
15. Competition between Sec- and TAT-dependent protein translocation in Escherichia coli
16. The role of the twin arginine motif in the signal peptide encoded by the hydA gene of the hydrogenase from Wolinella succinogenes
17. The efficient export of NADP-containing glucose-fructose oxidoreductase to the periplasm of Zymomonas mobilis depends both on an intact twin arginine motif in the signal peptide and on the generation of a structural export signal induced by cofactor binding
18. Site-directed mutagenesis of the hydrogenase signal peptide consensus box prevents export of a beta-lactamase fusion protein
19. The twin arginine consensus motif of Tat signal peptides is involved in Sec-independent protein targeting in Escherichia coli
20. A naturally occurring bacterial Tat signal peptide lacking one of the "invariant" arginine residues of the consensus targeting motif
21. Evidence against the double-arginine motif as the only determinant for protein translocation by a novel Sec-independent pathway in Escherichia coli
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23. Requirement for nickel of the transmembrane translocation of NiFe- hydrogenase 2 in Escherichia coli
24. A novel Sec-independent periplasmic protein translocation pathway in Escherichia coli
25. Ralstonia eutropha TF93 is blocked in tat-mediated protein export
26. Translocation of jellyfish green fluorescent protein via the Tat system of Escherichia coli and change of its periplasmic localization in response to osmotic up-shock
27. Processing of colicin V-1, a secretable marker protein of a bacterial ATP binding cassette export system, requires membrane integrity, energy, and cytosolic factors
28. Colicin V-treated Escherichia coli does not generate membrane potential
29. Genetic analysis of the colicin V secretion pathway
30. Escherichia coli strains blocked in Tat-dependent protein export exhibit pleiotropic defects in the cell envelope
31. A family of bacteriocin ABC transporters carry out proteolytic processing of their substrates concomitant with export
32. Subunit interactions in the twin arginine translocase complex of Escherichia coli
33. Purified components of the Escherichia coli Tat protein transport system form a double-layered ring structure
34. TatB and TatC form a functional and structural unit of the twin- arginine translocase from Escherichia coli
35. Functional reconstitution of bacterial Tat translocation in vitro
36. Green fluorescent protein functions as a reporter for protein localization in Escherichia coli
37. Export of Thermus thermophilus alkaline phosphatase via the twin arginine translocation pathway in Escherichia coli
38. Sec-independent protein translocation by the maize Hcf106 protein
39. Component specificity for the thylakoidal Sec and Delta pH-dependent protein transport pathways
40. The maize tha4 gene functions in sec-independent protein transport in chloroplasts and is related to hcf106, tatA, and tatB
41. A SecY homologue is required for the elaboration of the chloroplast thylakoid membrane and for normal chloroplast gene expression
42. New method for generating deletions and gene replacements in Escherichia coli
43. BAD promoter
44. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension