A pH-dependent conformational change, rather than the chemical step, appears to be rate-limiting in the hammerhead ribozyme cleavage reaction

Journal of Molecular Biology

Volumn 315, Issue 2, 2002, Pages 121-130

  Murray, James B ^a   Dunham, Christine M ^a   Scott, William G ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Catalytic RNA; Conformational change; Hammerhead ribozyme; Mechanism of catalysis; pH dependence

Indexed keywords

COBALT; HAMMERHEAD RIBOZYME; OXYGEN; RIBOZYME; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME CONFORMATION; ENZYME DEGRADATION; ENZYME KINETICS; ENZYME MECHANISM; ERROR; PH; PRIORITY JOURNAL; RNA SEQUENCE; X RAY DIFFRACTION;

EID: 0036293201     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5145     Document Type: Article

References (22)

1. Autolytic processing of dimeric plant virus satellite RNA
2. A small catalytic oligoribo-nucleotide
3. Simple RNA enzymes with new and highly specific endoribonuclease activities
4. Crystallographic structures of the hammerhead ribozyme: Relationship to ribozyme folding and catalysis
5. RNA sstructure and catalysis
6. Role of divalent metal ions in the hammerhead RNA cleavage reaction
7. Evidence for the role of solvated metal hydroxide in the hammerhead cleavage mechanism
8. The hammerhead, hairpin and VS ribozymes are catalytically proficient in monovalent cations alone
9. Comparison of the hammerhead cleavage reactions stimulated by monovalent and divalent cations
10. The hammerhead cleavage reaction in monovalent cations
11. Hammerhead ribozyme kinetics
12. Kinetic characterization of two I/II format hammer-head ribozymes
13. Hammerhead ribozymes with a faster cleavage rate
14. Three-dimensional structure of a hammerhead ribozyme
15. The crystal-structure of an all-RNA hammerhead ribozyme - A proposed mechanism for RNA catalytic cleavage
16. Capturing the structure of a catalytic RNA intermediate: The hammerhead ribozyme
17. The structural basis of hammerhead ribozyme self-cleavage
18. Capture and visualization of a catalytic RNA enzyme-product complex using crystal lattice trapping and X-ray holographic reconstruction
19. Structure and function of the hammerhead ribozyme: An unfinished story
20. Conventional and time-resolved ribozyme X-ray crystallography
21. New parameters for the refinement of nucleic acid-containing structures