Quantitative real-time PCR: A critique of method and practical considerations

Hematology

Volumn 7, Issue 1, 2002, Pages 59-67

  Ong, Yong Lee ^a   Irvine, Alexandra ^b  

^a BELFAST CITY HOSPITAL   (United Kingdom)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Perkin Elmer ABI PRISM 7700 sequence detection system; Quantitative real time PCR

Indexed keywords

EXONUCLEASE; FLUOROCHROME;

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENE SEQUENCE; HISTORY; INSTRUMENTATION; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; POLYMERIZATION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION;

EID: 0036287624     PISSN: 10245340     EISSN: None     Source Type: Journal    
DOI: 10.1080/10245330290015573     Document Type: Article

References (21)

1. "Real time quantitative PCR"
2. "Advances in quantitative PCR technology: 5′ nuclease assays"
3. "Developments in quantitative PCR"
4. "Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions"
5. "Detection of specific polymerase chain reaction product by utilizing the 5′ to 3′ exonuclease activity of Thermus aquaticus DNA polymerase"
6. "Allelic discrimination by nick-translation PCR with fluorogenic probes"
7. "Zwischenmolekulare Energie-wanderung und Fluoreszenz"
8. "Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization"
9. "Isolation and complete sequence of a functional glyceraldehyde-3-phosphate dehydrogenase gene"
10. "Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose"
11. "Detection of specific sequences among DNA fragments separated by gel electrophoresis"
12. "Novel 5′ exonuclease-based real-time PCR assay for the detection of t(14;18)(q32;q21) in patients with follicular lymphoma"
13. "Real-time fluorescence detection of a single DNA molecule"
14. "Detection of minimal residual disease in patients with AML1/ETO-associated acute myeloid leukemia using a novel quantitative reverse transcription polymerase chain reaction assay"
15. "Investigation of minimal residual disease in childhood and adult acute lymphoblastic leukemia by molecular analysis"
16. "Real-time RT-PCR for the detection and quantification of AML1/MTG8 fusion transcripts in t(8;21) positive AML patients"
17. "A quantitative reverse transcriptase polymerase chain reaction method for the detection of leukaemic cells with t(8;21) in peripheral blood"
18. "Quantitation of minimal residual disease in t(8;21)-positive acute myelogenous leukemia patients using real-time quantitative RT-PCR"
19. "Comparison of nested competitive RT-PCR and real-time RT-PCR for the detection and quantification of AML1/MTG8 fusion transcripts in t(8;21) positive acute myelogenous leukemia"
20. "The assessment of bcl-2 and bax in acute myeloid leukaemia: A comparison of immunocytochemistry and real-time PCR"
21. "Detection of methylthioadenosine phosphorylase (MTAP) and p16 gene deletion in T cell acute lymphoblastic leukaemia by real-time quantitative PCR assay"