Comparison of PCR and microscopic methods for detecting Trypanosoma cruzi

Journal of Clinical Microbiology

Volumn 34, Issue 5, 1996, Pages 1171-1175

  Kirchhoff, Louis V ^a,b,c   Votava, Julie R ^a,c,d   Ochs, Diane E ^a,b   Moser, David R ^a,b  

^a UNIVERSITY OF IOWA   (United States)

^b UNIVERSITY OF IOWA   (United States)

^c VETERANS AFFAIRS MEDICAL CENTER   (United States)

^d YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ANIMAL MODEL; ARTICLE; BLOOD SAMPLING; CHAGAS DISEASE; DIAGNOSTIC ACCURACY; INTERMETHOD COMPARISON; LABORATORY DIAGNOSIS; MICROBIOLOGICAL EXAMINATION; MICROSCOPY; MOUSE; NONHUMAN; PARASITISM; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTOZOON; TRYPANOSOMA CRUZI;

EID: 0029868633     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.5.1171-1175.1996     Document Type: Article

References (18)

1. Avila, H. A., J. B. Pereira, O. Thiemann. E, de Paiva, W. Degrave, C. M. Morel, and L. Simpson. 1993. Detection of Trypanosomu cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of kinetoplast minicircle DNA: comparison with serology and xenodiagnosis J. Clin. Microbiol. 31:2421-2426.
2. Avila, H. A., D. S. Sigman, L. M. Cohen, R. C. Millikan, and L. Simpson. 1991. Polymerase chain reaction amplification of Trypanosoma cruzi kinetoplast minicircle DNA isolated from whole blood lysates' diagnosis of chronic Chagas' disease. Mol. Biochem. Parasital. 48:211-222.
3. Britto, C., M. A. Cardoso, C. M. Monteiro Vanni, A. Hasslocher-Moreno, S. S. Xavier, W. Oelemann, A. Santoro, C. Pirmez, C. M. Morel, and P. Wincker. 1995. Polymerase chain reaction detection of Trypanosoma end in human blood samples as a tool for diagnosis and treatment evaluation. Parasitology 110:241-247.
4. Diaz, C., V. Nussenzweig, and A. Gonzalez. 1992. An improved polymerase chain reaction assay to detect Trypanosoma cruzi in blood. Am. J. Trop Med. Hyg. 46:616-623.
5. Hoft, D. F., R. G. Lynch, and L. V. Kirchhoff. 1993. Kinetic analysis of antigen specific immune responses in resistant and susceptible mice during infection with Trypanosoma cruzi. J. Immunol. 151:7038-7047
6. Kirchhoff, L. V. 1993. American trypanosomiasis (Chagas' disease) - a tropical disease now in the United States. N. Engl. J. Med. 329:639-644.
7. Kirchhoff, L. V., A. A. Gam, R. D. Gusmao, R. S. Goldsmith, J. M. Rezende, and A. Rassi. 1987. Increased specificity of serodiagnosis of Chagas' disease by detection of antibody to the 72- and 90-kilodalton glycoproteins of Trypanosoma cruzi. J. Infect. Dis. 155:561-564.
8. Kirchhoff, L. V., and D. F. Hoft. 1990. Immunization and challenge of mice with insect-derived metacyclic trypomastigotes of Trypanosoma cruzi. Parasite Immunol. 12:65-74.
9. Moser, D. R., L. V. Kirchhoff, and J. E. Donelson. 1989. Detection of Ttypanosoma cruzi by polymerase chain reaction gene amplification. J. Clin. Microbiol. 27:1744-1749.
10. Otsu, K., J. E. Donelson, and L. V. Kirchhoff. 1993. Interruption of a Trypanosoma cruzi gene encoding a protein containing 14-amino acid repeats by targeted insertion of the neomycin phosphotransferase gene. Mol. Biochem. Parasitol. 57:317-330.
11. Persing, D. H., and G. Cimino. 1993. Amplification product inactivation methods, p. 105-121 In D. H. Persing, F C. Tenover, T F Smith, and T. J. White (ed.), Diagnostic molecular biology: principles and applications. American Society for Microbiology. Washington, D C.
12. Pizzi, T. 1957. Inmunología de la enfermedad de Chagas. Thesis. University of Chile, Santiago.
13. Postan, M., A. W. Cheever, J. A. Dvorak, and J. P. McDaniel. 1986. A histopathological analysis of the course of myocarditis in C3H/He mice infected with Trypanosoma cruzi clone Sylvio-X10/4 Trans. R. Soc. Trop. Med. Hyg. 80:50-55.
14. Rowland, E. C., and D. M. Ritter. 1984. Corpus Christi strain-induced protection to Trypanosoma cruzi infection in C3H(He) mice: transfer of resistance to Brazil strain challenge with lymphocytes, J. Parasitol. 70:760-766.
15. Russomando, G., A. Figueredo, M. Almiron, M. Sakamoto, and K. Morita. 1992. Polymerase chain reaction-based detection of Trypanosoma cruzi DNA in serum. J. Clin. Microbiol. 30:2864-2868.
16. Sturm, N. R., W. Degrave, C. Morel, and L. Simpson. 1989. Sensitive detection and schizodeme classification of Trypanosoma cruzi cells by amplification of kinetoplast minicircle DNA sequences: use in diagnosis of Chagas' disease. Mol. Biochem. Parasitol. 33:205-214.
17. Wincker, P., C. Britto, J. B. Pereira, M. A. Cardoso, W. Oelemann, and C. M. Morel. 1994. Use of a simplified polymerase chain reaction procedure to detect Trypanosoma cruzi in blood samples from chronic chagasic patients in a rural endemic area. Am. J. Trop. Med. Hyg 51:771-777.
18. Zeledon, R., J. C. P. Dias, A. Brilla-Salazar, J. M. de Rezende, L. G. Vargas, and A. Urbina. 1988. Does a spontaneous cure for Chagas disease exist? Rev. Soc. Bras. Med. Trop. 21:15-20.