A chemiluminescence immunoassay for evaluation of Cryptosporidium parvum growth in vitro

FEMS Microbiology Letters

Volumn 136, Issue 3, 1996, Pages 251-256

  You, Xiangdong ^a   Arrowood, Michael J ^b   Lejkowski, Marisa ^a   Xie, Longti ^b   Schinazi, Raymond F ^a,c   Mead, Jan R ^a,c  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b NATL CENTER FOR INFECTIOUS DISEASES   (United States)

^c Georgia VA Res Ctr AIDS HIV I   (United States)

Author keywords

Cell culture; Chemiluminescence immunonssay; Cryptosporidium parvum; Drug evaluation; In vitro

Indexed keywords

MADURAMICIN; PAROMOMYCIN;

ANTIMICROBIAL ACTIVITY; ANTIPROTOZOAL ACTIVITY; ARTICLE; CELL CULTURE; CHEMOLUMINESCENCE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; DRUG RESEARCH; DRUG SCREENING; IMMUNOASSAY; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA; CRYPTOSPORIDIUM PARVUM;

EID: 0029975920     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1097(96)00004-3     Document Type: Article

References (14)

1. O'Donoghue, P.J. (1995) Cryptosporidium and cryptosporidiosis in man and animals. Int. J. Parasitol. 25, 139-195.
2. Flanigan, T., Aji, T., Marshall, R., Soave, R., Aikawa, M. and Kaetzel, C. (1991) Asexual development of Cryptosporidium parvum within a differentiated human enterocyte cell line. Infect. Immun. 59, 234-239.
3. Buraud, M., Forget, E., Favennec, L., Bitzet, J., Govert, J.-G. and Deluol, A.-M. (1991) Sexual stage development of cryptosporidia in the Caco-2 cell line. Infect. Immun. 59, 4610-4613.
4. Gut, J., Petersen, C., Nelson, R. and Leech, J. (1991) Cryptosporidium parvum: in vitro cultivation in Madin-Darby canine kidney cells. J. Protozool. 38, 72S-73S.
5. Arrowood, M.J., Xie, L. and Hurd, M. (1994) In vitro assays of maduramicin activity against Cryptosporidium parvum. J. Euk. Microbiol. 41, 23S.
6. Favennec, L., Egraz-Bernard, M., Comby, E., Lemeteil, D., Ballet, J.-J. and Brasseur, P. (1994) Immunofluorescence detection of Cryptosporidium parvum in Caco-2 cells: a new screening method for anticryptosporidial agents. J. Euk. Microbiol. 41, 39S.
7. Woods, K.M., Nesterenko, M.V., Upton, S.J. (1995) Development of a microtitre ELISA to quantify development of Cryptosporidium parvum in vitro. FEMS Microbiol. Lett. 128, 89-94.
8. Kricka, L.J. (1991) Chemiluminescent and bioluminescent techniques. Clin. Chem. 37, 1472-1481.
9. Arrowood, M.J. and Sterling, C.R. (1987) Isolation of Cryptosporidium oocyst and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J. Parasitol. 73, 314-319.
10. Belen'kii, M. and Schinazi, R.F. (1994) Multiple drug effect analysis with confidence interval. Antiviral Res. 25, 1-11.
11. Dyke, K.V. and Dyke, R.V. (1990) Luminescence Immunoassay and Molecular Applications, pp. 61-62. CRC Press, Boca Raton, FL.
12. Kricka, L.J. (1993) Ultrasensitive immunoassay techniques. Clin. Biochem. 26, 325-331.
13. Rehg, J.E. (1994) A comparison of anticryptosporidial activity of paromomycin with that of other aminoglycosides and azithromycin in immunosuppressed rats. J. Infect. Dis. 170, 934-938.
14. Mead, J.R., You, X., Pharr, J.E., Belenkaya, Y., Arrowood, M.J., Fallen, M.T. and Schinazi, R.F. (1995) Evaluation of maduramicin and alborixin in a SCID mouse model of chronic cryptosporidiosis. Antimicrob. Agents Chemother. 39, 854-858.