Probing FinO-FinP RNA interactions by site-directed protein-RNA crosslinking and gelFRET

RNA

Volumn 8, Issue 6, 2002, Pages 816-823

  Ghetu, Alexandru F ^a   Arthur, David C ^a   Kerppola, Tom K ^a   Glover, J N Mark ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Conjugation; Protein RNA interactions; Site specific crosslinking

Indexed keywords

GENE PRODUCT; PROTEIN FINO; PROTEIN FINP; RNA; UNCLASSIFIED DRUG;

ARTICLE; CROSS LINKING; ENERGY TRANSFER; FLUORESCENCE; PLASMID; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN RNA BINDING; PROTEIN STRUCTURE; RNA PROBE; RNA STRUCTURE;

BACTERIAL PROTEINS; BASE SEQUENCE; CROSS-LINKING REAGENTS; DNA PRIMERS; ESCHERICHIA COLI PROTEINS; F FACTOR; MOLECULAR PROBES; POINT MUTATION; REPRESSOR PROTEINS; RNA; RNA-BINDING PROTEINS;

EID: 0036269981     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838202026730     Document Type: Article

References (24)

1. Phenyl-azide-mediated photocross-linking analysis of Cro-DNA interaction
2. Molecular basis of cooperative DNA bending and oriented heterodimer binding in the NFAT1-Fos-Jun-ARRE2 complex
3. The nature of the transfer inhibitor of several F-like plasmids
4. Analysis of the sequence and gene products of the transfer region of the F sex factor
5. Crystal structure of the bacterial conjugation repressor FinO
6. The FinO repressor of bacterial conjugation contains two RNA binding regions
7. Directed cleavage of RNA with proteintethered EDTA-Fe
8. Sitedirected mutagenesis by overlap extension using the polymerase chain reaction
9. In vitro analysis of the interaction between the FinO protein and FinP antisense RNA of F-like conjugative plasmids
10. Degradation of FinP antisense RNA from F-like plasmids: The RNA-binding protein, FinO, protects FinP from ribonuclease E
11. Repression and derepresslon of conjugation of plasmid R1 by wild-type and mutated finP antisense RNA
12. The FinOP repressor system of plasmid R1: Analysis of the antisense RNA control of traJ expression and conjugative DNA transfer
13. FinOP repression of the F plasmid involves extension of the half-life of FinP antisense RNA by FinO
14. DNA bending determines Fos-Jun heterodimer orientation
15. Gene transfer between distantly related bacteria
16. Promoters in the transfer region of plasmid F
17. Determination of the orientation of a DNA binding motif in a protein-DNA complex by photocrosslinking
18. Gel-based fluorescence resonance energy transfer (gelFRET) analysis of nucleoprotein complex architecture
19. Long-range electrostatic interactions influence the orientation of Fos-Jun binding at AP-1 sites
20. Dynamics of Fos-JunNFAT1 complexes
21. Control of the orientation of Fos-Jun binding and the transcriptional cooperativity of Fos-Jun-NFAT1 complexes
22. Analysis of the major domains of the F-fertility inhibition protein, FinO
23. The FinO protein of IncF plasmids binds FinP antisense RNA and its target, traJ mRNA, and promotes duplex formation
24. Structural and functional analyses of the FinP antisense RNA regulatory system of the F conjugative plasmid