The FinOP repressor system of plasmid R1: Analysis of the antisense RNA control of traJ expression and conjugative DNA transfer

Molecular Microbiology

Volumn 21, Issue 4, 1996, Pages 811-821

  Koraimann, Günther ^a   Teferle, Karin ^a   Markolin, Gertrude ^a   Woger, Wolfgang ^a   Högenauer, Gregor ^a  

^a UNIVERSITY OF GRAZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY RNA; MESSENGER RNA;

ARTICLE; BACTERIUM CONJUGATION; CONTROLLED STUDY; DNA TRANSFER; GENE EXPRESSION REGULATION; NONHUMAN; PLASMID; PRIORITY JOURNAL; BACTERIAL STRAIN; ESCHERICHIA COLI; FINO GENE; FINP GENE; GENE ACTIVITY; GENE DOSAGE; GENE FUSION; GENE INTERACTION; GENE MAPPING; GENE REPRESSION; GENE TARGETING; POINT MUTATION; REGULATOR GENE; REPRESSOR GENE; SITE DIRECTED MUTAGENESIS; TRAJ GENE;

BACTERIA (MICROORGANISMS); ENTEROBACTERIACEAE;

EID: 0029745991     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.361401.x     Document Type: Article

References (37)

1. Ausubel, P.M., Brent, R., Kingston, R.E., Moore, D.D., Smith, J.A., Seidman, J.G., Struhl, K. (1987) Current Protocols in Molecular Biology. John Wiley and Sons, New York.
2. Balbas, P., Soberon, X., Merino, E., Zurita, M., Lomeli, H., Valle, F., Flores, N., and Bolivar, F. (1986) Plasmid vector pBR322 and its special purpose derivatives - a review. Gene 50: 3-40.
3. van Biesen, T., and Frost, L.S. (1992) Differential levels of fertility inhibition among F-like plasmids are related to the cellular concentration of finO mRNA. Mol Microbiol 6: 771-780.
4. van Biesen, T., and Frost, L.S. (1994) The FinO protein of IncF plasmids binds FinP antisense RNA and its target, traJ mRNA, and promotes duplex formation. Mol Microbiol 14: 427-436.
5. van Biesen, T., Soderbom, F., Wagner, E.G.H., and Frost, L.S. (1993) Structural and functional analyses of the FinP antisense RNA regulatory system of the F-conjugative plasmid. Mol Microbiol 10: 35-43.
6. Casadaban, M.J., and Cohen, S.N. (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol 138: 179-207.
7. Cheah, K.C., and Skurray, R. (1986) The F plasmid carries an IS3 insertion within finO. J Gen Microbiol 132: 3269-3275.
8. Cram, D.S., Loh, S.M., Cheah, K.C., and Skurray, R.A. (1991) Sequence and conservation of genes at the distal end of the transfer region on plasmids F and R6-5. Gene 104: 85-90.
9. Crooke, S.T., and Lebleu, B. (1993) Antisense Research and Application. Boca Raton: CRC Press.
10. Dempsey, W.B. (1989) Sense and antisense transcripts of traM, a conjugal transfer gene of the antibiotic resistance plasmid R100. Mol Microbiol 3: 561-570.
11. Dempsey, W.B. (1994) traJ sense RNA initiates at two different promoters in R100-1 and forms two stable hybrids with antisense finP RNA. Mol Microbiol 13: 313-326.
12. Eguchi, Y., Itoh, T., and Tomizawa, J. (1991) Antisense RNA. Annu Rev Biochem 60: 631-652.
13. Emory, S.A., and Belasco, J.G. (1990) The ompA 5′ untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency. J Bacteriol 172: 4472-4481.
14. Falkow, S. (1975) Infectious Multiple Drug Resistance. Pion Limited, London.
15. Frost, L., Lee, S., Yanchar, N., and Paranchych, W. (1989) finP and fisO mutations in FinP antisense RNA suggest a model for FinOP action in the repression of bacterial conjugation by the F lac plasmid JCFLO. Mol Gen Genet 218: 152-160.
16. Frost, L.S., Ippen-Ihler, K., and Skurray, R.A. (1994) Analysis of the sequence and gene products of the transfer region of the F sex factor. Microbiol Rev 58: 162-210.
17. Hawkins, J.W., and Nellen, W. (1994) Diversification of antisense research and development: review of the Ringberg meeting, April 1994. Mechanisms of antisense-mediated gene silencing. Antisense Res Dev 4: 127-129.
18. Hjalt, T.A., and Wagner, E.G.H. (1995) Bulged-out nucleotides in an antisense RNA are required for rapid target RNA binding in vitro and inhibition in vivo. Nucl Acids Res 23: 580-587.
19. Koraimann, G., Koraimann, C., Koronakis, V., Schlager, S., and Högenauer, G. (1991) Repression and derepression of conjugation of plasmid R1 by wild-type and mutated finP antisense RNA. Mol Microbiol 5: 77-87.
20. Koraimann, G., Schroller, C., Graus, H., Angerer, D., Teferle, K., and Högenauer, G. (1993) Expression of gene 19 of plasmid R1 is controlled by RNase III. Mol Microbiol 9: 717-727.
21. Lee, S.H., Frost, L.S., Paranchych, W. (1992) FinOP repression of the F plasmid involves extension of the half-life of FinP antisense RNA by FinO. Mol Gen Genet 235: 131-139.
22. Meynell, E., and Datta, N. (1967) Mutant drug resistant factors of high transmissibility. Nature 214: 885-887.
23. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
24. Minton, N.P. (1984) Improved plasmid vectors for the isolation of translational lac gene fusions. Gene 31: 269-273.
25. Mullineaux, P., and Willetts, N.S. (1985) Promoters in the transfer region of plasmid F. In Plasmids in Bacteria. Helinski, D.R., Cohen, S.N., Clewell, D.B., Jackson, D.A., and Hollaender, A. (eds). New York: Plenum Publishing Corporation, pp. 605-614.
26. Neckers, L., Whitesell, L., Rosolen, A., and Geselowitz, D.A. (1992) Antisense inhibition of oncogene expression. Crit Rev Oncog 3: 175-231.
27. Nellen, W., and Lichtenstein, C. (1993) What makes a messenger RNA anti-sensitive? Trends Biochem Sci 18: 419-423.
28. Predki, P.P., Nayak, L.M., Gottlieb, M.B., and Regan, L. (1995) Dissecting RNA-protein interactions: RNA-RNA recognition by Rop. Cell 80: 41-50.
29. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
30. Schwab, M., Gruber, H., and Högenauer, G. (1991) The TraM protein of plasmid R1 is a DNA-binding protein. Mol Microbiol 5: 439-446.
31. Silhavy, T.J., Berman, M.L., and Enquist, L.M. (1984) Experiments with Gene Fusions. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
32. Silverman, P.M., Wickersham, E., and Harris, R. (1991) Regulation of the F-plasmid traY promoter in Escherichia coli by host and plasmid factors. J Mol Biol 218: 119-128.
33. Vieira, J., and Messing, J. (1987) Production of single-stranded plasmid DNA. Meth Enzymol 153: 3-11.
34. Wagner, E.G.H., and Simons, R.W. (1994) Antisense RNA control in bacteria, phages and plasmids. Annu Rev Microbiol 48: 713-742.
35. Wagner, E.G.H., Blomberg, P., and Nordstrom, K. (1992) Replication control in plasmid R1: duplex formation between the antisense RNA, CopA, and its target, CopT, is not required for inhibition of RepA synthesis. EMBO J 11: 1195-1203.
36. Willetts, N. (1977) The transcription control of fertility in F-like plasmids. J Mol Biol 112: 41-48.
37. Yoshioka, Y., Ohtsubo, H., and Ohtsubo, E. (1987) Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO. J Bacteriol 169: 619-623.