Cloning and expression and purification of Hepatitis B e-antigen precursor in Escherichia coli

Chinese Medical Journal

Volumn 115, Issue 5, 2002, Pages 722-725

  Zhou, Fuyuan ^a   Sui, Lili ^a   Luo, Kangxian ^a   Hou, Jinlin ^a  

^a FIRST MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

HBeAg precursor; Hepatitis B virus; pQE30

Indexed keywords

CYTOPLASM PROTEIN; HEPATITIS B(E) ANTIBODY; HEPATITIS B(E) ANTIGEN; RECOMBINANT ANTIGEN;

ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ANTIGENICITY; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE SEQUENCE; HEPATITIS B; HEPATITIS B VIRUS; LIVER CELL; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PATHOGENESIS; PRECURSOR; PROMOTER REGION; TRANSCRIPTION REGULATION; WESTERN BLOTTING;

CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; HEPATITIS B E ANTIGENS; PLASMIDS; PROTEIN PRECURSORS; RECOMBINANT PROTEINS;

EID: 0036266416     PISSN: 03666999     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. The hepatitis B virus
2. Induction of T-helper cell response to hepatitis B core antigen in chronic hepatitis B: A major factor in activation of the host immune response to the hepatitis B virus
3. Specigicity of T lymphocyte cytotoxicity to autologous hepatocytes in chronic hepatitis B virus infection: Evidence that T cells are directed against HBV core antigen expressed on hepatocytes
4. Cellular immune response to hepatitis B virus-encoded antigens in acute and chronic hepatitis B virus infection
5. Immunochemical structure of hepatitis B e antigen in the serum
6. Nucleotide sequence of the hepatitis B virus genome cloned in E. coli
7. Precore-mediated inhibition of hepatitis B virus progeny DNA synthesis
8. Formation of rransmemdbraneous hepstitis B e-antigen by co-translational in vitro processing of the viral precore protein
9. Genetic variation in the cleavage site of the precore region of hepatitis B virus in Chinese patients with fulminant hepatitis
10. Hepatitis B virus gene function: The precore region targets the core antigen to cellular membranes and causes the secretion of the e antigen
11. Posttranscriptional regulation of hepatitis B virus replication by the precore protein
12. Structure of hepatitis B virus core and e-antigen. A single precore amino acid prevents nucleocapsid assembly
13. Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus
14. Production of hepatitis B virus preS polypeptide in escherichia coli by mutation of the 5′-end coding sequence and its purification and characterization
15. Cloning and expression in Escherichia coli of the recombinant His-tagged DNA polymerases from Pyrococcus furiosus and Pyrococcus woesei
16. 2+-chelate affinity chromatography