High-sensitivity hybridization assay for quantitation of residual E. coli DNA

BioTechniques

Volumn 32, Issue 5, 2002, Pages 1162-1167

  Ji, Xiaoying ^a   Lee, Kwan ^a   DiPaolo, Byron ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELL MEMBRANES; ESCHERICHIA COLI; GENETIC ENGINEERING; PROTEINS; SENSITIVITY ANALYSIS;

RECOMBINANT PROTEIN THERAPEUTICS;

DNA;

BACTERIAL DNA; DIGOXIGENIN; RECOMBINANT PROTEIN;

ARTICLE; CHEMOLUMINESCENCE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DNA DETERMINATION; DNA PROBE; ESCHERICHIA COLI; FOOD AND DRUG ADMINISTRATION; GENETIC RECOMBINATION; HOST CELL; HYBRIDIZATION; ISOTOPE LABELING; NONHUMAN; QUALITY CONTROL; QUANTITATIVE ANALYSIS; SAFETY; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0036255104     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02325dd06     Document Type: Article

References (9)

1. Using a CCD camera imaging system as a recording device to quantify human DNA by slot-blot hybridization
2. Validation of a chemiluminescent hybridization assay for quantitative determination of host cell DNA in clarified conditioned medium
3. Quantitation of residual E. coli DNA in recombinant biopharmaceutical proteins by hybridization analysis
4. Hybridization analysis for the quantitative determination of residual DNA in some recombinant protein expressed in E. coli cells
5. Calibration and LOD/LOQ estimation of a chemiluminescent hybridization assay for residual DNA in recombinant protein drugs expressed in E. coli using a four parameter logistic model
6. Detection of picogram amounts of nucleic acid by dot blot hybridization
7. Determining residual DNA levels: Comparison of hybridization with an alternative method
8. Reassessing the control of residual DNA in biopharmaceuticals