Ligand discovery using the inter-ligand Overhauser effect: Horse liver alcohol dehydrogenase

Biotechnology Letters

Volumn 24, Issue 8, 2002, Pages 623-629

  Li, Dawei ^a   London, Robert E ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

Alcohol dehydrogenase; Inter ligand Overhauser effect; Methylbenzamide; NMR; Sodium cyanoborohydride

Indexed keywords

ALCOHOL DEHYDROGENASE; BENZAMIDE DERIVATIVE; ENZYME INHIBITOR; LIGAND; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; SODIUM BOROHYDRIDE;

ARTICLE; COMPLEX FORMATION; ENZYME ANALYSIS; ENZYME INHIBITION; HORSE; MODEL; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; NUCLEAR OVERHAUSER EFFECT;

EQUUS CABALLUS;

EID: 0036240110     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1015095308654     Document Type: Article

References (26)

1. Small molecular conformation in the receptor-bound state by the two-dimensional spin exchange experiment
2. Reduction of nicotinamide adenine dinucleotides by sodium cyanoborohydride
3. A link between protein structure and enzyme catalyzed hydrogen tunneling
4. The conformation of coenzyme A bound to chloramphenicol acetyltransferase determined by transferred NOE experiments
5. +-pyrazole complex as determined by N-15 NMR Spectroscopy
6. Cd-113 NMR in binary and ternary complexes of cadmium-substituted horse liver alcohol dehydrogenase
7. Flexibility of liver alcohol dehydrogenase in stereoselective binding of 3-butylthiolane 1-oxides
8. Binding of substrate in a ternary complex of horse liver alcohol dehydrogenase
9. + bound to yeast and horse liver alcohol-dehydrogenase in solution - The use of the proton-proton transferred nuclear Overhause enhancement
10. Transient kinetic studies of substrate inhibition in the horse liver alcohol dehydrogenase reaction
11. Zim-modified cyanoborohydride as a selective reducing agent
12. The inter-ligand Overhauser effect: A powerful new NMR approach for mapping structural relationships of macromolecular ligands
13. Inter-ligand Overhauser effects in Type II dihydrofolate reductase. Biochemistry
14. Theoretical analysis of the inter-ligand Overhauser effect, a new approach for mapping structural relationships of macromolecular ligands
15. Development and evaluation of a boronate inhibitor of γ-glutamyl transpeptidase
16. Rapid recording of 2D NMR spectra without phase cycling. Application to the study of hydrogen exchange in proteins
17. Structures of horse liver alcohol dehydrogenase complexed with NAD(+) and substituted benzyl alcohols
18. Binding of formamides to liver alcohol dehydrogenase
19. The reaction of glutathione with amino acids and related compounds as catalyzed by γ-glutamyl transpeptidase
20. Electronic, hydrophobic, and steric effects of binding of inhibitors to the horse liver alcohol dehydrogenase-reduced pyridine coenzyme binary complex
21. The binding of 1,10-phenanthroline to specifically active-site cobalt(II)-substituted horse liver alcohol dehydrogenase - A probe for the open-enzyme conformation
22. Inhibition of human alcohol dehydrogenases by formamides
23. Interactions of substrates, inhibitors, and coenzymes at the active site of horse liver alcohol dehydrogenase
24. Binding of ligands to the catalytic zinc ion in horse liver alcohol dehydrogenase
25. On the effects of some heterocyclic compounds on the enzymic activity of liver alcohol dehydrogenase
26. Effect of solution pH and composition on horse liver alcohol dehydrogenase thermostability