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Volumn 24, Issue 8, 2002, Pages 657-662

Production of a functional dihydrofolate synthase with a cleavable poly-His tag in Saccharomyces cerevisiae

Author keywords

Dihydrofolate synthase; Poly His; Protein purification; Thrombin cleavage; Yeast expression system

Indexed keywords

DIHYDROFOLATE SYNTHETASE; POLYHISTIDINE; SYNTHETASE; UNCLASSIFIED DRUG;

EID: 0036237181     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1015051526401     Document Type: Article
Times cited : (4)

References (20)
  • 5
    • 0022354535 scopus 로고
    • Thrombin specificity. Requirement for apolar amino acids adjacent to the thrombin cleavage site of polypeptide substrate
    • (1985) Eur. J. Biochem. , vol.151 , pp. 217-224
    • Chang, J.Y.1
  • 6
    • 0032806779 scopus 로고    scopus 로고
    • Affinity purification of Schistosoma japonicum glutathione-S-transferase and its site-directed mutants with glutathione affinity chromatography and immobilized metal affinity chromatography
    • (1999) J. Chromatogr. , vol.A852 , pp. 151-159
    • Chen, H.M.1    Luo, S.L.2    Chen, K.T.3    Lii, C.K.4
  • 10
    • 0025977037 scopus 로고
    • Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase
    • (1991) Anal. Biochem. , vol.192 , pp. 262-267
    • Guan, K.L.1    Dixon, J.E.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.