Principles and practices for treatment of cutaneous wounds with cultured skin substitutes

American Journal of Surgery

Volumn 183, Issue 4, 2002, Pages 445-456

  Boyce, Steven T ^a   Warden, Glenn D ^a,b  

^a UNIVERSITY OF CINCINNATI   (United States)

^b SHRINERS HOSPITALS FOR CHILDREN   (United States)

Author keywords

Burns; Cultured skin; Skin grafts; Wound closure

Indexed keywords

ANTIINFECTIVE AGENT; BIOPOLYMER;

ARTICLE; BURN; DONOR; HISTOGENESIS; HUMAN; MORPHOGENESIS; ORGANOGENESIS; POSTOPERATIVE COMPLICATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SKIN AUTOGRAFT; SKIN CELL; SKIN CULTURE; SKIN INJURY; SKIN PIGMENTATION; TISSUE ENGINEERING; WOUND CARE; WOUND CLOSURE; WOUND INFECTION;

BIOCOMPATIBLE MATERIALS; BURNS; CELLS, CULTURED; FORECASTING; HUMANS; KERATINOCYTES; SKIN; SKIN PHYSIOLOGY; SKIN TRANSPLANTATION; SKIN, ARTIFICIAL; SOFT TISSUE INJURIES; TISSUE ENGINEERING;

EID: 0036227481     PISSN: 00029610     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0002-9610(02)00813-9     Document Type: Article

References (110)

1. Prevention and treatment of postburn scars and contracture
2. Biomechanical alterations in normal skin and hypertrophic scar after thermal injury
3. Epidermiology, demographic and outcome characteristics of burn injury
4. Recent outcomes in the treatment of burn injury in the United States: A report from the American Burn Association patient registry
5. Burn incidence and medical care use in the United States: Estimates, trends, and data sources
6. Expression of an exogenous growth hormone gene in transplantable human epidermal cells
7. Targeted expression of insulin-like growth factor to human keratinocytes: Modification of the autocrine control of keratinocyte proliferation
8. Genetically modified skin to treat disease: Potentials and limitations
9. Systemic delivery of secreted protein by grafts of epidermal keratinocytes: Prospects for keratinocyte gene therapy
10. Enhanced vascularization of cultured skin substitutes genetically modified to overexpress vascular endothelial growth factor
11. Aggressive use of free flaps in children for burn scar contractures and other soft-tissue deficits
12. Longitudinal trapezius fasciocutaneous flap for the treatment of mentosternal burn scar contractures
13. Tissue expansion in children: A retrospective study of complications
14. Epidermal lipids, barrier function, and desquamation
15. Stratum corneum architecture, metabolic activity and interactivity with subjacent cell layers
16. Human keratinocytes contain carbohydrates that are recognized by keratan sulfate-specific monoclonal antibodies
17. The biochemistry and role of epidermal lipid synthesis
18. Lipid metabolism in cultured keratinocytes
19. Characterization of growth hormone enhanced donor site healing in patients with large cutaneous burns
20. Differential expression and localization of insulin-like growth factors I and II in cutaneous wounds of diabetic and nondiabetic mice
21. Management of pediatric facial burns
22. The mesh skin autograft
23. Functional outcomes for the patient with burn injuries
24. Characteristics and uses of biologic dressings and skin substitutes
25. Addendum: Multicenter experience with cultured epithelial autografts for treatment of burns
26. New techniques for the in vitro culture of human skin keratinocytes and perspectives on their use for grafting of patients with extensive burns
27. Skin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers
28. Lack of long term durability of cultured keratinocyte burn wound coverage: A case report
29. Cultured epithelial autograft: Five years of clinical experience with twenty-eight patients
30. Wound closure and outcome in extensively burned patients treated with cultured autologous keratinocytes
31. Skin repair with the cultured cells and biopolymers
32. Cultured skin substitutes: A review
33. Permanent coverage of large burn wounds with autologous cultured human epithelium
34. Skin regenerated from cultured epithelial autografts on full-thickness burn wounds from 6 days to 5 years after grafting
35. Cultured epithelial autografts: Three years experience with eighteen patients
36. Comparison of cultured epidermal autograft and massive excision with serial autografting plus homograft overlay
37. Cultured epidermal autografts: A life-saving and skin-saving technique in children
38. Composite autologous-allogeneic skin replacement: Development and clinical application
39. Clinical trials of a living dermal tissue replacement placed beneath meshed, split-thickness skin grafts on excised wounds
40. The organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function
41. Transplantation of human melanocytes
42. Effect of recombinant IGF binding protein-1 on primary cultures of human keratinocytes and fibroblasts: Selective enhancement of IGF-1 but not IGF-2-induced cell proliferation
43. Pigmentation and inhibition of wound contraction by cultured skin substitutes with adult melanocytes after transplantation to athymic mice
44. Regulation of pigmentation in cultured skin substitutes by cytometric sorting of melanocytes and keratinocytes
45. Quantitative threshold changes in cutaneous sensation of patients with burns
46. Sensory loss over grafted areas in patients with burns
47. Human hair follicle regeneration following amputation and grafting into the nude mouse
48. Gene expression of insulin-like growth factor-1 and IGF-1 receptor during wound healing in rats
49. Cultured skin for wound closure
50. The 1999 Clinical Research Award. Cultured skin substitutes combined with Integra to replace native skin autograft and allograft for closure of full-thickness burns
51. Acceleration of skin regeneration from cultured epithelial autografts by transplantation to homograft dermis
52. Cutaneous wound repair
53. Cells, matrix, growth factors, and the surgeon: The biology of scarless fetal wound repair
54. Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture
55. Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells
56. Cultivation, frozen storage, and clonal growth of normal human epidermal keratinocytes in serum-free media
57. Biologic attachment, growth, and differentiation of cultured human epidermal keratinocytes on a graftable collagen and chondroitin-6-sulfate substrate
58. Characterization, barrier function and drug metabolism of an in vitro skin model
59. Epidermis generated in vitro: Practical consideration and applications
60. Lipid supplemented medium induces lamellar bodies and precursors of barrier lipids in cultured analogues of human skin
61. Methods for cultivation of keratinocytes at the air-liquid interface
62. Triglyceride metabolism in human keratinocytes cultured at the air-liquid interface
63. Loss of requirement for exogenous epidermal growth factor by cultured analog of human skin
64. Use of a serum-free epidermal culture model to show deleterious effects of epidermal growth factor on morphogenesis and differentiation
65. Dual control of cell growth by somatomedins and platelet-derived growth factor
66. PDGF and FGF stimulate wound healing in the genetically diabetic mouse
67. Enchancement of wound healing by topical treatment with epidermal growth factor
68. The safety and efficacy of topically applied recombinant basic fibroblast growth factor on the healing of chronic pressure sores
69. Effects of the platelet-derived growth factor/insulin-like growth factor-I combination on bone regeneration around titanium dental implants: Results of pilot study in beagle dogs
70. Artifical dermis for major burns; A multi-center randomized clinical trial
71. Successful use of a physiologically acceptable skin in the treatment of extensive burn injury
72. Current treatment recommendations for topical burn therapy
73. In vitro toxicity of topical antimicrobial agents to human fibroblasts
74. Cellular amd bacterial toxicities of topical antimicrobials
75. Evaluation of synthetic wound dressing capable of releasing silver sulfadiazine
76. Selection of topical antimicrobial agents for cultured skin for burns by combined assessment of cellular cytotoxicity and antimicrobial activity
77. Cytotoxicity testing of topical antimicrobial agents on human keratinocytes and fibroblasts for cultured skin grafts
78. Non-cytotoxic combinations of topical antimicrobial agents for use with cultured skin
79. The wet disc antimicrobial solution assay: An in vitro method to test efficacy of antimicrobial solutions for topical use
80. Cultured epidermis for coverage of burn wounds: A single center experience
81. Clinical evaluation of an acellular allograft dermal matrix in full-thickness burns
82. Wound healing potential of cultured epithelium
83. Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate
84. The use of cultured autologous epidermis in the treatment of extensive burn wounds
85. Insulin-like growth factors-1 and -2 and their role in the re-epithelialization of wounds; Interactions with insulin like growth factor binding protein type 1
86. The skin
87. The 1996 Clinical Research award. Use of a pneumatonometer in burn scar assessment
88. Rating the burn scar
89. Cultured skin substitutes reduce donor skin harvesting for closure of excised, full-thickness burns
90. Surface electrical capacitance as an index of epidermal barrier properties of composite skin substitutes and skin autografts
91. FDA and EEC regulations related to skin documentation and measuring devices
92. Definitions and guidelines for assessment of wounds and evaluation of healing
93. Treatment of chronic wounds with cultured cells and biopolymers
94. Genetically modified human epidermis overexpressing PDGF-A directs the development of a cellular and vascular connective tissue stroma when transplanted to athymic mice-implications for the use of genetically modified keratinocytes to modulate dermal regeneration
95. Genetic modification of cultured skin substitutes by transduction of human keratinocytes with PDGF-A
96. Systematic distribution of apolipoprotein E secreted by grafts of epidermal keratinocytes: Implications for epidermal function and gene therapy
97. Genetically modified keratinocytes transplanted to wounds reconstitute the epidermis
98. A model for keratinocyte gene therapy: Preclinical and therapeutic considerations
99. Epidermis as a secretory tissue
100. Surface electrical capacitance as a non-invasive index of epidermal barrier in cultured skin substitutes in athymic mice
101. Early assessment of pediatric burns by laser doppler flowmetry
102. A portable scanning spectrophotometer using wavelengths for the rapid measurements of skin pigments
103. Quantitative assessment of UV-induced pigmentation and erythema
104. Mechanical properties and Youngs modulus of human skin in vivo
105. Cultured epithelial autografting on meshed skin graft scars: Evaluation of skin elasticity
106. The application of laser surface scanning for quantifying human wound progression
107. History, precedent, and progress in the development of mammalian cell culture systems for preparing vaccines: Safety considerations revisited
108. The new generation of bichemical polymers
109. Biomedical polymers