Glyco-Western blotting: Biotinylated dermatan sulfate as a probe for the detection of dermatan sulfate binding proteins using western blotting

Connective Tissue Research

Volumn 43, Issue 1, 2002, Pages 1-7

  Saito, A ^a   Munakata, H ^b   Satoh, K ^b  

^a KINKI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

Author keywords

Avidin Biotin; Biotinylated dermatan sulfate; Western blotting

Indexed keywords

ALKALINE PHOSPHATASE; AVIDIN; BINDING PROTEIN; BIOTIN; BOVINE SERUM ALBUMIN; BUFFER; CHONDROITIN 4 SULFATE; CORE PROTEIN; DERMATAN SULFATE; PYROXYLIN;

ARTICLE; ARTIFICIAL MEMBRANE; BIOTINYLATION; CELL CULTURE; CELL LYSATE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME SUBSTRATE; HUMAN; HUMAN CELL; LUNG ALVEOLUS CELL; MOLECULAR PROBE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; WESTERN BLOTTING;

BOVINAE;

EID: 0036227452     PISSN: 03008207     EISSN: None     Source Type: Journal    
DOI: 10.1080/gye.43.1.1.7     Document Type: Article

References (28)

1. Proteoglycans: Structures and interactions
2. Proteoglycans of the extracellular environment: Clues from the gene and protein side offer novel perspective in molecular diversity and function
3. Matrix proteoglycans: From molecular design to cellular function
4. Regulated diversity of heparan sulfate
5. Targeting the glycosaminoglycan-binding sites on proteins
6. Glycosaminoglycan-protein interactions: Definition of consensus sites in glycosaminoglycan binding proteins
7. Leucine-rich repeat glycoproteins of the extracellular matrix
8. Activation of heparin cofactor II by dermatan sulfate
9. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
10. Very-high-resolution two-dimensional electrophoretic separation of proteins on giant gel
11. Biotinylated hyaluronan: A versatile and highly sensitive probe capable of detecting nanogram levels of hyaluronan binding proteins (hyaladherins) on electroblots by a novel affinity detection procedure
12. Biotinylated hyaluronic acid: A new tool for probing hyaluronate-receptor interactions
13. A modified uronic acid carbazole reaction
14. Two-dimensional electrophoresis with immobilized pH gradient gel strip (in Japanese)
15. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
16. Mucopolysaccharides of skin
17. A method for the separation of acid mucopolysacharides: Its application to the isolation of heparin from the skin of rats
18. Influence of core protein sequence on glycosaminoglycan assembly
19. 3H]Acetyl-labeling, a convenient method for radiolabeling of glycosaminoglycans
20. Molecular characterization of Xenopus embryo heparan sulfate: Differential structural requirements for the specific binding to basic fibroblast growth factor and follistatin
21. Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe
22. Detection of aggregatable proteoglycan populations by affinity blotting using biotinylated hyaluronan
23. Antibody-affinity blotting, a sensitive technique for the detection of α-fetoprotein separated by lectin affinity electrophoresis in agarose gels
24. Negative regulation of transforming growth factor-beta by the proteoglycan decorin
25. Nucleotide sequence of mouse HSP60 (chaperonin, GroEL homolog) cDNA
26. Homologous plant and bacterial proteins chaperone oligomeric protein assembly