Determination of the mitotic index by microinjection of fluorescently labelled tubulin

European Journal of Cell Biology

Volumn 81, Issue 3, 2002, Pages 169-174

  Schwindling, Sandra L ^a   Faust, Michael ^a   Montenarh, Mathias ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

Fluorescently labelled tubulin; Immunofluorescence; Microinjection; Mitosis; Spindle

Indexed keywords

ALPHA TUBULIN; BETA TUBULIN; FLUORESCENT DYE;

ANIMAL CELL; ARTICLE; ASSAY; CELL CYCLE; CELL GROWTH; CELL POPULATION; CONTROLLED STUDY; MAMMAL CELL; MEASUREMENT; METHODOLOGY; MICROINJECTION; MITOSIS; MITOSIS INDEX; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS;

ANIMALIA; MAMMALIA;

EID: 0036226902     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1078/0171-9335-00235     Document Type: Article

References (17)

1. 2 arrest after DNA damage
2. Temporal and spatial control of cyclin B1 destruction in metaphase
3. Casein kinase II catalyses a mitotic phosphorylation on threonine 1342 of human DNA topoisomerase IIα which is recognized by the 3F3/2 phosphoepitope antibody
4. Monoclonal antibodies to mitotic cells
5. A new culture model facilitating rapid quantitative testing of mitotic spindle inhibition in mammalian cells
6. Cell-cycle modulation of MPM-2-specific spindle pole body phosphorylation in Aspergillus nidulans
7. Mitotic phosphorylation of DNA topoisomerase II α by protein kinase CK2 creates the MPM-2 phosphoepitope on Ser-1469
8. Cytoplasmic accumulation of cdc25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells
9. cdc25 is a nuclear protein expressed constitutively throughout the cell cycle in nontransformed mammalian cells
10. Polo-like kinases: A team that plays throughout mitosis
11. Entry into mitosis without Cdc2 kinase activation
12. The cell cycle
13. Flow cytometric discrimination of mitotic cells: Resolution of M, as well as G1, S, and G2 phase nuclei with mithramycin, propidium iodide, and ethidium bromide after fixation with formaldehyde
14. Mitotic effects of a constitutively active mutant of the Xenopus Polo-like kinase P1×1
15. Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-α tubulin
16. Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos
17. Fluorescent localisation of contractile proteins in tissue culture cells