Functional assembly of the foreign carotenoid lycopene into the photosynthetic apparatus of Rhodobacter sphaeroides, achieved by replacement of the native 3-step phytoene desaturase with its 4-step counterpart from Erwinia herbicola

Molecular Microbiology

Volumn 44, Issue 1, 2002, Pages 233-244

  Garcia Asua, Guillermo ^a   Cogdell, Richard J ^b   Hunter, C Neil ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; BACTERIOCHLOROPHYLL; CAROTENOID; LYCOPENE; OXYGENASE; PHYTOENE DESATURASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; CATALYSIS; ENERGY ABSORPTION; ENERGY RESOURCE; ENZYME REPLACEMENT; ERWINIA; ERWINIA HEBICOLA; FATTY ACID DESATURATION; LIGHT ABSORPTION; NONHUMAN; PHOTOSYNTHESIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN FUNCTION; PROTEIN STABILITY; RHODOBACTER SPHAEROIDES;

ANTIOXIDANTS; CAROTENOIDS; CELL MEMBRANE; DNA PRIMERS; ERWINIA; GENE EXPRESSION REGULATION, BACTERIAL; OXIDOREDUCTASES; PHOTOSYNTHESIS; PLASMIDS; RHODOBACTER SPHAEROIDES;

BACTERIA (MICROORGANISMS); ERWINIA; NEGIBACTERIA; PANTOEA AGGLOMERANS; PHOTOBACTERIA; RHODOBACTER; RHODOBACTER SPHAEROIDES; RHODOPSEUDOMONAS;

EID: 0036225748     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2002.02871.x     Document Type: Article

References (37)

1. Structure and sequence of the photosynthesis gene cluster
2. Purification and biochemical characterization of a hydroxyneurosporene desaturase involved in the biosynthetic pathway of the carotenoid spheroidene in Rhodobacter sphaeroides
3. Absorption spectral shifts of carotenoids related to medium polarizability
4. Conserved enzymes mediate the early reactions of carotenoid biosynthesis in non-photosynthetic and photosynthetic prokaryotes
5. ξ-carotene biosynthesis by extracts of the C115 mutant of Phycomyces blakesleeanus
6. Worked examples of isolation and analysis, example 3: Bacteria
7. Energy-transfer between the carotenoid and the bacteriochlorophyll within the B800-850 light-harvesting pigment-protein complex of Rhodopseudomonas-Sphaeroides
8. Localized transposon Tn5 mutagenesis of the photosynthetic gene cluster of Rhodobacter sphaeroides
9. Carotenoids
10. - mutant of Rhodobacter sphaeroides
11. Mechanism of energy transfer from carotenoids to bacteriochlorophyll: Light harvesting by carotenoids having different extents of pi electron conjugation incorporated into the B850 antenna complex from the carotenoidless bacterium Rhodobacter sphaeroides R26-1
12. Photosensitized oxidation and singlet oxygen: Consequences in biological systems
13. -10 in the B800 bacteriochlorophyll and carotenoid pigment environment within the light harvesting LH2 complex of Rhodobacter sphaeroides
14. Expression in Escherichia coli, purification, and reactivation of the recombinant Erwinia uredovora phytoene desaturase
15. Carotenoid diversity: A modular role for the phytoene desaturase step
16. The gene crtI mediates the conversion of phytoene into colored carotenoids in Rhodopseudomonas capsulata
17. The carotenoid shift in Rhodopseudomonas sphaeroides. Change induced under continuous illumination
18. Functional assignment of Erwinia herbicola Eho10 carotenoid genes expressed in Escherichia coli
19. Transfer of genes coding for apoproteins of reaction center and light-harvesting LH1 complexes to Rhodobacter sphaeroides
20. Introduction of new carotenoids into the bacterial photosynthetic apparatus by combining the carotenoid biosynthetic pathways of Erwinia herbicola and Rhodobacter sphaeroides
21. The crystal structure of the light-harvesting complex II (B800-B850) from Rhodospirillum molischanum
22. A null lesion in the rhodopin 3,4-desaturase of Rhodospirillum rubrum unmasks a cryptic branch of the carotenoid biosynthetic pathway
23. The relationship between carotenoid biosynthesis and the assembly of the light-harvesting LH2 complex in Rhodobacter sphaeroides
24. Early steps in carotenoid biosynthesis: Sequence and transcriptional analysis of the crtI and crtB genes of Rhodobacter sphaeroides. Overexpression and reactivation of crtI. Escherichia coli and Rhodobacter sphaeroides
25. Complete DNA sequence, specific Tn5 insertion map, and gene assignment of the carotenoid biosynthesis pathway of Rhodobacter sphaeroides
26. Efficient energy transfer from carotenoid S2 state in a photosynthetic light-harvesting complex
27. Accumulation of carotenoids in structural and regulatory mutants of the bacterium Myxococcus xanthus
28. Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria
29. The photosynthesis gene cluster of Rhodobacter sphaeroides
30. Modification of a hydrogen bond between a bound chromophore and the α subunit of the light-harvesting LH1 antenna of Rhodobacter sphaeroides
31. Pleiotropic effects of puf interposon mutagenesis on carotenoid biosynthesis in Rubrivivax gelatinosus: A new gene organization in purple bacteria
32. In vitro caroteno-genesis and characterisation of the phytoene desaturase reaction in Anacystis
33. Substrate specificity of the expressed carotenoid 3,4 desaturase from Rubrivivax gelatinosus reveals the detailed reaction sequence to spheroidene and spirilloxanthin
34. Projection structures of three photosynthetic complexes from Rhodobacter sphaeroides: LH2 at 6 Å, LH1 and RC-LH1 at 25 Å
35. Alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution
36. A structural role of the carotenoid in the light-harvesting II protein of Rhodobacter capsulatus