Radiolysis of lac repressor by γ-rays and heavy ions: A two-hit model for protein inactivation

Biophysical Journal

Volumn 82, Issue 5, 2002, Pages 2373-2382

  Charlier, Michel ^a   Eon, Séverine ^a   Sèche, Édouard ^a   Bouffard, Serge ^b   Culard, Françoise ^a   Spotheim Maurizot, Mélanie ^a  

^a CENTRE DE BIOPHYSIQUE MOLÉCULAIRE   (France)

^b UNIVERSITÉ DE CAEN   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; DIMER; HEAVY ION; PEPTIDE; REPRESSOR PROTEIN; TYROSINE;

ARTICLE; DNA BINDING; GAMMA RADIATION; GENE INACTIVATION; LACTOSE OPERON; NONHUMAN; OXIDATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN BINDING; RADIOLYSIS;

EID: 0036224897     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(02)75582-3     Document Type: Article

References (27)

1. DNA-induced conformational changes in cyclic AMP receptor protein: Detection and mapping by a protein footprinting technique using multiple chemical proteases
2. Repressor recognition of operator and effectors
3. A closer view of the conformation of the Lac repressor bound to operator
4. -) in aqueous solution
5. Lac repressor-lac operator interaction. Circular dichroism study
6. Protein damage and degradation by oxygen radicals. I. General aspects
7. Radiation disrupts protein-DNA complexes through damage to the protein. The lac repressor-operator system
8. Sequence of the lac I gene
9. La radiolyse de l'eau et des solutions aqueuses: Historique et actualité
10. How lac repressor find lac operator in vitro
11. Radiolytic footprinting. Beta rays, gamma photons, and fast neutrons probe DNA-protein interactions
12. Reaction mechanisms in the radiolysis of peptides, polypeptides and proteins
13. The nucleotide sequence of the lac operator
14. Mapping protein domains involved in macromolecular interactions: A novel protein footprinting approach
15. Nonspecific DNA binding of genome-regulating proteins as a biological control mechanism: Measurement of DNA-bound Escherichia coli lac repressor in vivo
16. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer
17. Millisecond radiolytic modification of peptides by synchrotron x-rays identified by mass spectrometry
18. Determination of macromolecular folding and structure by synchrotron x-ray radiolysis techniques
19. Radiation chemistry of biopolymers
20. The lacI gene: Its role in lac operon control and its use as a genetic system
21. The formation of strand breaks in DNA after high-LET irradiation: A comparison of data from in vivo and cellular systems
22. Refined structure of lac repressor headpiece (1-56) determined by relaxation matrix calculations from 2D and 3D NOE data: Change of tertiary structure upon binding to the lac operator
23. Hinge-helix formation and DNA bending in various lac repressor-operator complexes
24. Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions
25. Wrapping of flanking non-operator DNA in lac repressor-operator complexes: Implications for DNA looping
26. Lac repressor fragments produced in vivo and in vitro: An approach to the understanding of the interaction of repressor and DNA