Intracellular cysteines of the cystic fibrosis transmembrane conductance regulator (CFTR) modulate channel gating

Cellular Physiology and Biochemistry

Volumn 12, Issue 1, 2002, Pages 1-8

  Ketchum, Christian ^a   Yue, Hongwen ^a   Alessi, Karen ^a   Devidas, Shreenivas ^a   Guggino, William ^a   Maloney, Peter ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

ABC transporter; ATP binding cassette; CFTR; Channel gating; Cysteine mutagenesis; Open probability; Sulphydryl labeling

Indexed keywords

ABC TRANSPORTER; CHLOROMERCURIBENZENESULFONIC ACID; CYSTEINE; FORSKOLIN; MERCURY CHLORIDE; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; ARTICLE; CELL LINE; CHANNEL GATING; CHLORIDE CURRENT; CONTROLLED STUDY; HUMAN; HUMAN CELL; MUTAGENESIS; NONHUMAN; OOCYTE; PRIORITY JOURNAL; PROTEIN FUNCTION; XENOPUS LAEVIS;

XENOPUS LAEVIS;

EID: 0036206202     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000047821     Document Type: Article

References (29)

1. Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA
2. Generation of cAMP-activated chloride currents by expression of CFTR
3. Cyclic AMP-dependent protein kinase opens chloride channels in normal but not cystic fibrosis airway epithelium
4. Phosphorylation of the R domain by cAMP-dependent protein kinase regulates the CFTR chloride channel
5. Nucleoside triphosphates are required to open the CFTR chloride channel
6. Demonstration that CFTR is a chloride channel by alteration of its anion selectivity
7. The cystic fibrosis transmembrane conductance regulator chloride channel. Iodide block and permeation
8. Amino acid residues lining the chloride channel of the cystic fibrosis conductance regulator
9. Locating the anion-selectivity filter of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel
10. Channel-lining residues in the M3 membrane-spanning segment of the cystic fibrosis transmembrane conductance regulator
11. Construction of a functional lactose permease devoid of cysteine residues
12. Determination of transmembrane protein structure by disulphide cross-linking: The E. coli Tar receptor
13. Acetylcholine receptor channel structure probed in cysteine-substitution mutants
14. Identification of a residue in the translocation pathway of a membrane carrier
15. Protein folding - What's the problem?
16. Cysteine scanning mutagenesis of the putative transmembrane helices IX and X in the lactose permease of Escherichia coli
17. Amino acids lining the channel of the gamma-aminobutyric acid type A receptor identified by cysteine substitution
18. The second half of the cystic fibrosis conductance regulator forms a functional chloride channel
19. Both the wild type and a functional isoform of CFTR are expressed in kidney
20. Effect of deleting the R domain on CFTR-generated chloride channels
21. Function of the second nucleotide-binding fold in the CFTR chloride channel
22. A functional R domain from cystic fibrosis transmembrane conductance regulator is predominately unstructured in solution
23. Severed molecules functionally define the boundaries of the cystic fibrosis transmembrane conductance regulator's NH(2)-terminal nucleotide binding domain
24. The inhibition of metabolic oxalate production by sulphydryl compounds
25. Effect of thiol reagents on phosphoinositide hydrolysis in the rat brain
26. The role of glutathione S-transferase and cytochrome P450-dependent metabolism in the olfactory toxicity of methyl iodide in the rat
27. Melibiose carrier of Escherichia coli use of cysteine mutagenesis to identify the amino acids on the hydrophilic face of transmembrane helix 2
28. Molecular characterization of cystic fibrosis: 16 Novel mutations identified by analysis of the whole cystic fibrosis conductance regulator (CFTR) coding regions and splice site junctions