Solubilization of Na,K-ATPase from rabbit kidney outer medulla using only C12E8

Brazilian Journal of Medical and Biological Research

Volumn 35, Issue 3, 2002, Pages 277-288

  Santos, H L ^a   Lamas, R P ^a   Ciancaglini, P ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

( )2 dimer; C12E8; Membrane solubilization; Na,K ATPase; Rabbit kidney medulla

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; 3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 2 HYDROXY 1 PROPANESULFONIC ACID; 4 NITROPHENYL PHOSPHATE; ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); DETERGENT; DIMER; DODECYL SULFATE SODIUM; HYDROLASE; OLIGOMYCIN; OUABAIN; SEPHAROSE; VANADATE SODIUM;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL MEMBRANE; CHROMATOGRAPHY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; GEL FILTRATION; GEL FILTRATION CHROMATOGRAPHY; INTERMETHOD COMPARISON; KIDNEY MEDULLA; LOW TEMPERATURE; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; RABBIT; SOLUBILIZATION;

ANIMALIA; ORYCTOLAGUS CUNICULUS; STROPHANTHUS GRATUS;

EID: 0036205952     PISSN: 0100879X     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/S0100-879X2002000300002     Document Type: Article

References (40)

1. Properties of detergent
2. Methodological aspects of purification and reconstitution of transport proteins from mammalian plasma membranes
3. Solubilization and functional reconstitution of biomembrane components
4. A practical approach to the choice of a suitable detergent and optimal conditions to solubilize a membrane protein
5. Interaction of membrane proteins and lipids with solubilizing detergents
6. Functional reconstitution of the sodium pump. Kinetics of exchange reactions performed by reconstituted Na,K-ATPase
7. +)-ATPase
8. Asymmetric incorporation of Na,K-ATPase into phospholipid vesicles
9. Soluble and enzymatically stable Na,K-ATPase from mammalian kidney consisting predominantly of protomer αβ-units. Preparation, assay and reconstitution of active Na,K transport
10. + by the Na,K-ATPase in the presence of oligomycin
11. Functional domains of Na,K-ATPase; conformational transitions in the α-subunit and ion occlusion
12. The identification of the sodium-potassium pump
13. Structure and function of the Na,K pump: Ten years of molecular biology
14. Structure-function relationships based on ATP binding and cation occlusion at equilibrium in Na,K-ATPase
15. Transport-linked conformational changes in Na,K-ATPase. Structure-function relationships of ligand binding and E1-E2 conformational transitions
16. Ligand binding sites of Na,K-ATPase
17. Ouabain binding kinetics of the rat alpha two and alpha three isoforms of the sodium-potassium adenosine triphosphate
18. Insect Na,K-ATPase
19. Soluble and active renal Na,K-ATPase with maximum protein molecular mass 170,000 ± 9,000 daltons; formation of larger units by secondary aggregation
20. Solubilized αβ Na,K-ATPase remains protomeric during turnover yet shows apparent negative cooperativity toward ATP
21. Binding of 2′(3′)-O-(2,4,6-trinitrophenyl)ADP to soluble alpha beta protomers of Na,K-ATPase modified with fluorescein isothiocyanate. Evidence for two distinct sites
22. Photoin-activation of fluorescein isothiocyanate-modified Na,K-ATPase by 2′(3′)-O-(2,4,6-trinitrophenyl)8-azidoadenosine 5′-diphosphate
23. +-activated phosphatase reside on different alpha-subunits
24. Inhibitors of the Na,K-ATPase
25. Identification of a model cardiac glycosid receptor: Comparisons with Na,K-ATPase
26. E2P phosphoforms of Na,K-ATPase. I. Comparison of phosphointermediates formed from ATP and Pi by their reactivity toward hydroxylamine and vanadate
27. Purification of Na,K-ATPase. Enzyme sources, preparative problems and preparation from mammalian kidney
28. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein
29. Disc electrophoresis II - Method and application to human serum proteins
30. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
31. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
32. Sigraf-A versatile computer-program for fitting enzyme kinetic data
33. Consequences of mutations to the phosphorylation site of the alpha-subunit of Na,K-ATPase for ATP binding and E1-E2 conformational equilibrium
34. Purification of active Na,K-ATPase using a new ouabain-affinity column
35. Reconstitution of membrane proteins into liposomes: Application to energy-transducing membrane proteins
36. Structural basis for E1-E2 conformational transitions in Na,K-pump proteins
37. +-ATPase sensing ATP binding?
38. Substrate sites for the Na,K dependent ATPase
39. A two-site model of interacting ATP sites