Purification of active Na+-K+-ATPase using a new ouabain-affinity column

American Journal of Physiology - Cell Physiology

Volumn 275, Issue 4 44-4, 1998, Pages

  Yingst, Douglas R ^a   Yang, Shang You ^a   Schiebinger, Rick ^a  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Affinity chromatography; Dog kidney; Kinase; Phosphatase; Phosphorylation; Potassium; Rat adrenal glomerulosa; Rat kidney; Sodium

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); OUABAIN;

ANIMAL TISSUE; ARTICLE; DOG; ENZYME PHOSPHORYLATION; ENZYME PURIFICATION; KIDNEY MICROSOME; NONHUMAN; POTASSIUM TRANSPORT; PRIORITY JOURNAL; RAT; SODIUM TRANSPORT; ZONA GLOMERULOSA;

EID: 0031751812     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.1998.275.4.c1167     Document Type: Article

References (36)

1. Allen, J. C., and A. Schwartz. A possible biochemical explanation for the insensitivity of the rat to cardiac glycosides. J. Pharmacol. Exp. Ther. 168: 42-46, 1969.
2. Arystarkhova, E., and K. J. Sweadner. Isoform-specific monoclonal antibodies to Na,K-ATPase alpha subunits. Evidence for a tissue-specific post-translational modification of the alpha subunit. J. Biol. Chem. 271: 23407-23417, 1996.
3. Beguin, P., M. Peitsch, and K. Geering. Alpha 1 but not alpha 2 or alpha 3 isoforms of Na,K-ATPase are efficiently phosphorylated in a novel protein kinase C motif. Biochemistry 35: 14098-14108, 1996.
4. Blostein, R. Relationships between erythrocyte membrane phsphorylation and adenosine triphosphate hydrolysis. J. Biol. Chem. 243: 1957-1965, 1968.
5. Borghini, I., K. Geering, A. Gjinovci, C. B. Wollheim, and W.-F. Pralong. In vivo phosphorylation of the Na,K-ATPase α subunit in sciatic nerves of control and diabetic rats: effect of protein kinase modulators. Proc. Natl. Acad. Sci. USA 91: 6211-6215, 1994.
6. +-ATPase in response to beta-adrenergic hormone. Am. J. Physiol. 273 (Cell Physiol. 42): C893-C901, 1997.
7. Cortas, N., D. Elstein, D. Markowitz, and I. S. Edelman. Anomalous mobilities of Na,K-ATPase alpha subunit isoforms in SDS-PAGE: identification by N-terminal sequencing. Biochim. Biophys. Acta 1070: 223-228, 1991.
8. Craven, T. G., D. C. Kem, and R. J. Schiebinger. Atrial natriuretic peptides: the role of phenylalanine on biological activity. Endocrinology 122: 826-830, 1988.
9. Eschenbruch, M., and R. R. Burk. Experimentally improved reliability of ultrasensitive silver staining of protein in polyacrylamide gels. Anal. Biochem. 125: 96-99, 1982.
10. +-ATPase: mechanisms underlying rapid and sustained changes in pump activity. Am. J. Physiol. 269 (Cell Physiol. 38): C295-C311, 1995.
11. Feschenko, M. S., and K. J. Sweadner. Conformation-dependent phosphorylation of Na,K-ATPase by protein kinase A and protein kinase C. J. Biol. Chem. 269: 30436-30444, 1994.
12. Feschenko, M. S., and K. J. Sweadner. Phosphorylation of Na,K-ATPase by protein kinase C at Ser 18 occurs in intact cells but does not result in direct inhibition of ATP hydrolysis. J. Biol. Chem. 272: 17726-17733, 1997.
13. Forbush, B., III. Assay of Na,K-ATPase in plasma membrane preparations: increasing the permeability of membrane vesicles using sodium dodecyl sulfate buffered with bovine serum albumin. Anal. Biochem. 128: 159-163, 1983.
14. Glynn, I. M. The action of cardiac glycosides on sodium and potassium movements in human red cells. J. Physiol. (Lond.) 136: 148-173, 1957.
15. + pump in rat adrenal glomerulosa cells: possible contribution to stimulation of aldosterone production. Endocrinology 130: 1637-1644, 1992.
16. +)-activated ATPase. A biochemical link to digitalis-induced inotropy. Pharmacol. Rev. 36: 143-163, 1984.
17. +) activated ATPase from ox brain. Biochim. Biophys. Acta 311: 51-66, 1973.
18. Hoffman, J. F. The red cell membrane and the transport of sodium and potassium. Am. J. Med. 41: 666-680, 1966.
19. +-ATPase: a method for estimating the extent of inhibition of an enzyme sample containing an unknown amount of bound cardiac gycoside. Life Sci. 16: 1253-1261, 1975.
20. +)-ATPase. III. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecyl sulphate. Biochim. Biophys. Acta 356: 36-52, 1974.
21. Jorgensen, P. L. Isolation and characterization of the components of the sodium pump. Q. Rev. Biophys. 7: 239-274, 1975.
22. +-ATPase without altering the cation binding properties of the enzyme. J. Biol. Chem. 270: 16206-16212, 1995.
23. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685, 1970.
24. +-sensitive reaction: kinetic and spectroscopic studies. Arch. Biochem. Biophys. 140: 371-378, 1970.
25. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275, 1951.
26. Middleton, J. P. Direct regulation of the Na,K pump by signal transduction mechanisms. Miner. Electrolyte Metab. 22: 293-302, 1996.
27. Okarma, T. B., P. Tramell, and S. M. Kalman. Inhibition of sodium- and potassium-dependent adenosine triphosphate by digoxin covalently bound to Sepharose. Mol. Pharmacol. 8: 476-480, 1972.
28. Post, R. L., S. Kume, T. Tobin, B. Orcutt, and A. K. Sen. Flexibility of an active center in sodium-plus-potassium adenosine triphosphate. J. Gen. Physiol. 54 Suppl.: 306S-326S, 1969.
29. Retz, K. C., and W. J. Steele. Acid precipitation of protein in the presence of Triton X-100 and deoxycholate. Anal. Biochem. 79: 457-461, 1977.
30. Shen, B.-Q., C.-M. Yang, and J. H. Widdicombe. Rapid procedure for obtaining tracheal apical membranes. Am. J. Physiol. 261 (Lung Cell. Mol. Physiol. 5): L102-L105, 1991.
31. Skou, J. C. The Na,K-pump. Methods Enzymol. 156: 1-25, 1988.
32. Szamel, M., M. Goppelt-Strübe, W. Bessler, K. H. Wiesmüller, and K. Resch. Separation of plasma membrane domains of calf thymocytes by affinity chromatography on ouabain-Sepharose. Biochim. Biophys. Acta 899: 247-257, 1987.
33. +)-ATPase. Biochim. Biophys. Acta 274: 551-555, 1972.
34. +)ATPase. Biochim. Biophys. Acta 198: 120-131, 1970.
35. Yeh, L.-A., L. Ling, L. English, and L. Cantley. Phosphorylation of the (Na,K)-ATPase by a plasma membrane-bound protein kinase in friend erythroleukemia cells. J. Biol. Chem. 258: 6567-6574, 1983.
36. +)-ATPase of human red blood cells. Biochem. Biophys. Res. Commun. 111: 970-979, 1983.