Cochlear whole mount in situ hybridization: Identification of longitudinal and radial gradients

Brain Research Protocols

Volumn 9, Issue 1, 2002, Pages 65-76

  Judice, Tiffany N ^a   Nelson, Nathan C ^a   Beisel, Chase L ^a   Delimont, Duane C ^a   Fritzsch, Bernd ^b   Beisel, Kirk W ^a  

^a BOYS TOWN NATIONAL RESEARCH HOSPITAL   (United States)

^b CREIGHTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cochlea; Expression pattern; Hair cell; In situ hybridization; Nicotinic acetylcholine receptor 9; Otoferlin; Prestin

Indexed keywords

ANIMAL TISSUE; ARTICLE; CELLULAR DISTRIBUTION; COCHLEA; CONTROLLED STUDY; CORTI ORGAN; DOWN REGULATION; EPITHELIUM; GENE CONTROL; GENE EXPRESSION REGULATION; HAIR CELL; IN SITU HYBRIDIZATION; INNER EAR; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT; SIGNAL DETECTION;

NON-PROGRAMMATIC;

AGING; ANIMALS; ANTISENSE ELEMENTS (GENETICS); COCHLEA; DISSECTION; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HAIR CELLS; IN SITU HYBRIDIZATION; MEMBRANE PROTEINS; MICE; MICROSCOPY; PROTEINS; RATS; RECEPTORS, NICOTINIC;

EID: 0036178693     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(01)00138-6     Document Type: Article

References (23)

1. Characterization of G-protein βγ expression in inner ear
2. Longitudinal gradients of KCNQ4 expression in spiral ganglion and cochlear hair cells correlate with progressive hearing loss in DFNA2
3. Expression and localization of prestin and the sugar transporter GLUT-5 during development of electromotility in cochlear outer hair cells
4. Fluorescence in situ hybridization of scarce leptin receptor mRNA using the enzyme-labeled fluorescent substrate method and tyramide signal amplification
5. Alpha 9: An acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells
6. Spatial shaping of cochlear innervation by temporally-regulated neurotrophin expression
7. Mechanisms of hair cell tuning
8. Immunohistochemical localization of several cytoskeletal proteins in inner ear sensory and supporting cells
9. Expression of a potassium current in inner hair cells during development of hearing in mice
10. Transient expression of an inwardly rectifying potassium conductance in developing inner and outer hair cells along the mouse cochlea
11. Neurofilament proteins in avian auditory hair cells
12. The distribution of the calcium buffer calbindin in the cochlea of the guinea-pig
13. α and β subunits of acetylcholine receptors in the human inner ear
14. Development of sensory and neural structures in the mammalian cochlea
15. + channel isoforms along the tonotopic gradient of the chicken's cochlea
16. Preservation of mRNA during in situ hybridization in the cochlea
17. Differential expression of the alpha 9 nicotinic acetylcholine receptor subunit in neonatal and adult cochlear hair cells
18. Structure of the mammalian cochlea
19. Cryoembedding and sectioning of cochleas for immunocytochemistry and in situ hybridization
20. Polysialic acid in the cochlea of the developing mouse
21. OTOF encodes multiple long and short isoforms: Genetic evidence that the long ones underlie recessive deafness DFNB9
22. Prestin is the motor protein of cochlear outer hair cells
23. Visualization of α9 acetylcholine receptor expression in hair cells of transgenic mice containing a modified bacterial artificial chromosome