Determination of nelfinavir free drug concentrations in plasma by equilibrium dialysis and liquid chromatography/tandem mass spectrometry: Important factors for method optimization

European Journal of Pharmaceutical Sciences

Volumn 15, Issue 2, 2002, Pages 185-195

  Herforth, Claudia ^a   Stone, Judith A ^a   Jayewardene, Anura L ^a   Blaschke, Terrence F ^b   Fang, Fang ^b   Motoya, Toshiro ^c   Aweeka, Francesca T ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c KAGOSHIMA UNIVERSITY   (Japan)

Author keywords

Equilibrium dialysis; Free fraction; HIV; Nelfinavir; Plasma protein binding; Protease inhibitor

Indexed keywords

ABSORPTION; ASSAYS; CONCENTRATION (PROCESS); DIALYSIS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PLASMAS;

FREE FRACTIONS;

DRUG PRODUCTS;

NELFINAVIR; PROTEINASE INHIBITOR;

ABSORPTION; ARTICLE; BIOASSAY; BLOOD SAMPLING; CONTROLLED STUDY; DRUG ADSORPTION; DRUG BLOOD LEVEL; DRUG DETERMINATION; EQUILIBRIUM DIALYSIS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; TANDEM MASS SPECTROMETRY; VALIDATION PROCESS;

ADSORPTION; CALIBRATION; CHEMISTRY, PHYSICAL; CHROMATOGRAPHY, LIQUID; DIALYSIS; HIV PROTEASE INHIBITORS; HUMANS; HYDROGEN-ION CONCENTRATION; MASS SPECTROMETRY; MOLECULAR STRUCTURE; NELFINAVIR; PROTEIN BINDING; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 0036175426     PISSN: 09280987     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0928-0987(01)00220-2     Document Type: Article

References (19)

1. Failure to detect nelfinavir in the cerebrospinal fluid (CSF) of HIV-1 infected patients with and without AIDS dementia complex
2. HIV-protease inhibitors
3. Importance of protein binding for the interpretation of serum or plasma drug concentrations
4. Serum protein pattern in normal pregnancy with special reference to acute-phase reactants
5. An LC/tandem MS method for the determination of indinavir, an HIV-1 protease inhibitor, in human plasma
6. Drug binding to human alpha-1-acid glycoprotein in health and disease
7. Rationale for monitoring free drug levels
8. Determination of unbound drug concentration and protein-drug binding fraction in plasma
9. Solubility, partition, and adsorption of digitalis glycosides
10. Comparative evaluation of microultrafiltration devices for determination of protein binding
11. Alpha 1-acid glycoprotein levels in AIDS patients before and after short-term treatment with zidovudine (ZDV)
12. Drug-protein binding sites. New trends in analytical and experimental methodology
13. Nelfinavir mesylate: A protease inhibitor
14. Plasma protein binding and therapeutic drug monitoring
15. Drug protein binding in chronic renal failure: Evaluation of nine drugs
16. Extraction of subnanomole amounts of amines and quaternary ammonium compounds from albumin solutions and human blood serum
17. Changes in plasma drug binding and alpha 1-acid glycoprotein in mother and newborn infant