Detection of tumor mutations in the presence of excess amounts of normal DNA

Nature Biotechnology

Volumn 20, Issue 2, 2002, Pages 186-189

  Sun, Xiyuan ^a   Hung, K ^a   Wu, L ^b   Sidransky, D ^b   Guo, Baochuan ^a  

^a CLEVELAND STATE UNIVERSITY   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; CELLS; DNA; IONIZATION; MASS SPECTROMETRY; PROTEINS; TUMORS;

MUTATIONS;

BIOTECHNOLOGY;

DNA; DNA FRAGMENT; GENOMIC DNA; PEPTIDE NUCLEIC ACID;

ARTICLE; CANCER SCREENING; EARLY DIAGNOSIS; GENE MUTATION; GENOME; HUMAN; HUMAN CELL; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TUMOR CELL;

ALLELES; CODON; DNA; DNA MUTATIONAL ANALYSIS; GENES, P53; HUMANS; LUNG NEOPLASMS; MUTATION; NEOPLASMS; POLYMERASE CHAIN REACTION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0036168249     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0202-186     Document Type: Article

References (20)

1. Colorectal cancer: Molecules and populations
2. Biomarkers for early detection of colon cancer
3. Early detection of lung cancer: Clinical perspectives of recent advances in biology and radiology
4. Nucleic acid-based methods for the detection of cancer
5. Molecular detection of tumor cells in bronchoalveolar lavage fluid from patients with early stage lung cancer
6. Detecting colorectal cancer in stool with the use of multiple genetic targets
7. Colorectal cancer screening by detection of altered human DNA in stool: Feasibility of a multitarget assay panel
8. Rapid p53 sequence analysis in primary lung cancer using an oligonucleotide probe array
9. Single base pair mutation analysis by PNA directed PCR clamping
10. Sensitive detection of p53 gene mutations by a "mutant enriched" PCR-SSCP technique
11. Facilitated detection of oncogene mutations from exfoliated tissue materials by a PNA-mediated "enriched PCR" protocol
12. Rapid determination of short DNA sequences by the use of MALDI-MS
13. Detecting CFTR gene mutations by using primer oligo base extension and mass spectrometry
14. A new MALDI-TOF based mini-sequencing assay for genotyping of SNPs
15. Methods for detection of point mutations: Performance and quality assessment
16. A highly sensitive assay for mutant ras genes and its application to the study of presentation and relapse genotypes in acute leukemia
17. Detection of K-ras oncogene mutation by polymerase chain reaction-based ligase chain reaction
18. Multiplex PCR/LDR for detection of K-ras mutations in primary colon tumors
19. Association between cigarette smoking and mutation of the p53 gene in squamous-cell carcinoma of the head and neck
20. A simplified procedure for developing multiplex PCRs