Effects of replacement of prolines with alanines on the catalytic activity and thermostability of inorganic pyrophosphatase from thermophilic bacterium PS-3

Journal of Biochemistry

Volumn 131, Issue 1, 2002, Pages 53-58

  Masuda, Hideki ^a   Uchiumi, Toshio ^a   Wada, Michiko ^a   Ichiba, Tetsuroh ^b   Hachimori, Akira ^a  

^a SHINSHU UNIVERSITY   (Japan)

^b OTSUKA PHARMACEUTICAL CO LTD   (Japan)

Author keywords

Inorganic pyrophosphatase; Mutagenesis; Polymerase chain reaction; Proline; Thermophilic bacterium PS 3

Indexed keywords

ALANINE; INORGANIC PYROPHOSPHATASE; MAGNESIUM CHLORIDE; PROLINE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CIRCULAR DICHROISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MUTAGENESIS; NONHUMAN; PH; POLYMERASE CHAIN REACTION; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

BACILLUS SP. PS3; BACTERIA (MICROORGANISMS);

EID: 0036167774     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003077     Document Type: Article

References (35)

1. On the metabolic significance of phosphorolytic and pyrophosphorolytic reactions
2. Microbial inorganic pyrophosphatase
3. Cytoplasmic inorganic pyrophosphatase
4. Pyrophosphatase is essential for growth of Escherichia coli
5. Yeast PPA2 gene encodes a mitochondrial inorganic pyrophosphatase that is essential for mitochondrial function
6. Expression of E. coli inorganic pyrophosphatase in transgenic plants alters photo-assimilate partitioning
7. Evolutionary aspect of inorganic pyrophosphatase
8. Cloning and expression of a unique inorganic pyrophosphatase from Bacillus subtilis: Evidence for a new family of enzymes
9. Bacillus subtilis ORF yybQ encodes a manganese-dependent inorganic pyrophosphatase with distinctive properties: The first of a new class of soluble pyrophosphatase?
10. 2+ ions against fluoride inhibition
11. Quaternary structure and metal ion requirement of Family II pyrophosphatase from Bacillus subtilis, Streptococcus gordonii and Streptococcus mutans
12. The structure of E. coli soluble inorganic pyrophosphatase at 2.7 Å resolution
13. Crystallographic identification of metalbinding sites in Escherichia coli inorganic pyrophosphatase
14. The structural basis for pyrophosphatase catalysis
15. X-ray structure of yeast inorganic pyrophosphatase complexed with manganese and phosphate
16. Crystal structure of holo inorganic pyrophosphatase from Escherichia coli at 1.9 Å resolution. Mechanism of hydrolysis
17. Structure and function analysis of Escherichia coli inorganic pyrophosphatase: Is a hydroxide ion the key to catalysis?
18. Evolutionary conservation of enzymatic catalysis: Quantitative comparison of the effects of mutation of aligned residues in Saccharomyces cerevisiae and Escherichia coli inorganic pyrophosphatases on enzymatic activity
19. Effect of D97E substitution on the kinetic and thermodynamic properties of Escherichia coli inorganic pyrophosphatase
20. Effect of E20D substitution in the active site of Escherichia coli inorganic pyrophosphatase on its quaternary structure and catalytic properties
21. 2+ binding
22. Escherichia coli inorganic pyrophosphatase: Site-directed mutagenesis of the metal binding sites
23. Structural and functional consequences of substitutions at the tyrosine 55-lysine 104 hydrogen bond in Escherichia coli inorganic pyrophosphatase
24. Primary structure of the inorganic pyrophosphatase from thermophilic bacterium PS-3
25. Cloning and expression of the inorganic pyrophosphatase gene from thermophilic bacterium PS-3
26. Effects of divalent cations on thermophilic inorganic pyrophosphatase
27. Effects of replacement of His-118, His-125 and Trp-143 by alanine on the catalytic activity and subunit assembly of inorganic pyrophosphatase from thermophilic bacterium PS-3
28. Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding
29. Sulfolobus acidcaldarius inorganic pyrophosphatase: Structure, thermostability, and effect of metal ion in an archael pyrophosphatase
30. Crystal structure of inorganic pyrophosphatase from Thermus thermophilus
31. DNA sequencing with chain-terminating inhibitors
32. Purification and characterization of inorganic pyrophosphatase from Bacillus stearothermophilus
33. Some observations on the role of copper ions in the reduction of phosphomolybdate by ascorbic acid and their application in the determination of inorganic phosphate
34. An unusual route to thermostability disclosed by the comparison of Thermus thermophilus and Escherichia coli inorganic pyrophosphatases
35. Hydrophobic interactions of Val-75 are critical for oligomeric thermostability of inorganic pyrophosphatase from Bacillus stearothermophilus