Preparation of recombinant RNase single-chain antibody fusion proteins

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 20, Issue 1, 2002, Pages 63-76

  Newton, Dianne L ^a   Rybak, Susanna M ^b  

^a SAIC FREDERICK INC   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

RNase; RNase chimeras; RNase fusion proteins; RNase immunofusion proteins; RNase sFv; Single chain antibody

Indexed keywords

HYBRID PROTEIN; RIBONUCLEASE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ARTICLE; BINDING KINETICS; CELL INCLUSION; CHROMATOGRAPHY; EXPRESSION VECTOR; FAST PROTEIN LIQUID CHROMATOGRAPHY; GENE CONSTRUCT; GENE SEQUENCE; GENETIC CODE; MOLECULAR CLONING; NONHUMAN; PLASMID; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN RENATURATION; SEQUENCE ANALYSIS; SOLUBILIZATION;

ANIMALS; ANTIBODIES; CHROMATOGRAPHY; HUMANS; IMMUNOCONJUGATES; NEOPLASMS; RECOMBINANT FUSION PROTEINS; RIBONUCLEASES;

VECTORS;

EID: 0036154938     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:20:1:063     Document Type: Article

References (45)

1. Gene splicing by overlay exension: Tailor made genes using the polymerase chain reaction
2. Use of T7 RNA polymerase to direct expression of cloned genes
3. Immunotoxins: Magic bullets or misguided missiles?
4. Immunotoxins against cancer
5. Immunotoxins: An update
6. Targeted therapy of cancer with recombinant immunotoxins
7. Human immune response to multiple injections of murine monoclonal IgG
8. Human anti-murine immunoglobulin response in patients receiving monoclonal antibody therapy
9. Clinical use of immunotoxins: Monoclonal antibodies conjugated to protein toxins
10. Toxicity and immunogenicity of monoclonal antimelanoma antibody-ricin A chain immunotoxins in rats
11. Human response to immunotoxins
12. Human immune response to monoclonal antibodies
13. Comprehensive pharmacokinetics of a humanized antibody and analysis of residual anti-idiotypic responses
14. Immunoenzymes
15. Molecular evolution of the ribonuclease superfamily
16. From housekeeper to microsurgeon: The diagnostic and therapeutic potential of ribonucleases
17. Natural and engineered cytotoxic ribonucleases: Therapeutic potential
18. Eosinophils, ribonucleases and host defense: Solving the puzzle
19. Amino acid sequence of human tumor derived angiogenin
20. Sequence of the cDNA and gene for angiogenin, a human angiogenesis factor
21. Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells
22. Angiogenin abolishes cell-free protein synthesis by specific ribonucleolytic inactivation of ribosomes
23. Comparison of RNases and Toxins upon injection into Xenopus oocytes
24. Cytotoxic potential of RNase and RNase hybrid proteins
25. Cytotoxic ribonuclease chimeras: Targeted tumoricidal activity in vitro and in vivo
26. Anti-tumor ribonuclease combined with or conjugated to monoclonal antibody MRK16, overcomes multidrug resistance to vincristine in vitro and in vivo
27. Potent and specific antitumor effects of an anti-CD22-targeted cytotoxic ribonuclease: Potential for the treatment of non-Hodgkin lymphoma
28. Epidermal growth factor receptor-dependent cytotoxic effect by an EGF-ribonuclease conjugate on human cancer cell lines: A trial for less immunogenic chimeric toxin
29. Human pancreatic RNase1-human epidermal growth factor fusion: An entirely human immunotoxin analog with cytotoxic properties against squamous cell carcinomas
30. Cloning and cytotoxicity of fusion proteins of EGF and angiogenin
31. Inhibition of cell growth by a fused protein of human ribonuclease 1 and human basic fibroblast growth factor
32. Humanization of immuntoxins
33. Antitransferrin receptor antibody-RNase fusion protein expressed in the mammary gland of transgenic mice
34. Expression and characterization of recombinant human eosinophil-derived neurotoxin anti-transferrin sFv
35. Cytotoxicity of a human pancreatic RNase A immunotoxin
36. Angiogenin single-chain immunofusions: Influence of peptide linkers and spacers between fusion protein domains
37. Functional properties of human ribonuclease fusion proteins expressed in Escherichia coli or transgenic mice
38. Single-chain-antigen-binding proteins
39. Protein engineering of single-chain Fv analogs and fusion proteins
40. A bifunctional fusion protein containing Fc-binding fragment B of staphylococcal protein A amino terminal to antidigoxin single-chain Fv
41. In vitro recombination and mutagenesis of DNA; SOEing together tailor-made genes
42. Interaction of rifamycin with bacterial RNA polymerase
43. Extraction of recombinant protein from bacteria
44. A method for increasing the yield of properly folded recombinant fusion proteins: Single chain immunotoxins from renaturation of bacterial inclusion bodies
45. Independent domain folding of Pseudomonas exotoxin and single-chain immunotoxins: Influence of interdomain connections