A Ca2+-dependent global conformational change in the 3D structure of phosphorylase kinase obtained from electron microscopy

Structure

Volumn 10, Issue 1, 2002, Pages 23-32

  Nadeau, Owen W ^a   Carlson, Gerald M ^a   Gogol, Edward P ^a  

^a UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

Author keywords

Ca2+; Electron microscopy; Phosphorylase kinase; Single particle analysis

Indexed keywords

CALCIUM ION; CALMODULIN; OLIGOMER; PHOSPHORYLASE KINASE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENZYME ACTIVATION; ENZYME ANALYSIS; ENZYME BINDING; ENZYME CONFORMATION; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBUNIT; FEMALE; GLYCOGENOLYSIS; NONHUMAN; PRIORITY JOURNAL; SKELETAL MUSCLE;

ANIMALS; CALCIUM; ENZYME ACTIVATION; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, ELECTRON; MODELS, MOLECULAR; PHOSPHORYLASE KINASE; PROTEIN STRUCTURE, QUATERNARY; PROTEIN SUBUNITS; RABBITS;

ANIMALIA;

EID: 0036153608     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(01)00678-5     Document Type: Article

References (36)

1. Phosphorylase kinase: The complexity of its regulation is reflected in the complexity of its structure
2. Molecular basis of signal integration in phosphorylase kinase
3. 2+-dependent modulator protein as the fourth subunit of rabbit skeletal muscle phosphorylase kinase
4. 2+
5. The role of calcium ions, calmodulin and troponin in the regulation of phosphorylase kinase from rabbit skeletal muscle
6. 2+/calmodulin on phosphorylase kinase
7. Effector-sensitive cross-linking of phosphorylase b kinase by the novel cross-linker 4-phenyl-1,2,4-triazoline-3,5-dione
8. Activators of phosphorylase kinase alter the cross-linking of its catalytic subunit to the C-terminal one-sixth of its regulatory α subunit
9. Antipeptide antibodies as probes of subunit-dependent structural changes in the regulatory domain of the gamma-subunit of phosphorylase kinase
10. Phosphorylase kinase conformers
11. The role of phosphorylase kinase in the nervous and hormonal control of glycogenolysis in muscle
12. Electron microscopical examination of different aggregation and decomposition states of phosphorylase kinase
13. Analyses of phosphorylase kinase by transmission and scanning transmission electron microscopy
14. Structure of phosphorylase kinase. A three-dimensional model derived from stained and unstained electron micrographs
15. Direct observation of phosphorylase kinase and phosphorylase b by scanning tunneling microscopy
16. Direct visualization of phosphorylase-phosphorylase kinase complexes by scanning tunneling and atomic force microscopy
17. The subunit structure of rabbit-skeletal-muscle phosphorylase kinase, and the molecular basis of its activation reactions
18. Escherichia coli 70 S ribosome at 15 Å resolution by cryo-electron microscopy: Localization of fMet-tRNAfMet and fitting of L1 protein
19. Three-dimensional structure of phosphorylase kinase at 22 Å resolution and its complex with glycogen phosphorylase b
20. An epitope proximal to the carboxyl terminus of the α-subunit is located near the lobe tips of the phosphorylase kinase hexadecamer
21. Proximal regions of the catalytic γ and regulatory β subunits on the interior lobe face of phosphorylase kinase are structurally coupled to each other and with enzyme activation
22. Self-association of the α subunit of phosphorylase kinase as determined by two-hybrid screening
23. Direct visualization of the calmodulin subunit of phosphorylase kinase via electron microscopy following subunit exchange
24. Isolation and physicochemical properties of active complexes of rabbit muscle phosphorylase kinase
25. Isolation and properties of the active γ subunit of phosphorylase kinase
26. Kinetic characterization of the calmodulin-activated catalytic subunit of phosphorylase kinase
27. The γ-subunit of skeletal muscle phosphorylase kinase contains two noncontiguous domains that act in concert to bind calmodulin
28. Purification and characterization of catalytic fragments of phosphorylase kinase gamma subunit missing a calmodulin-binding domain
29. Activated states of phosphorylase kinase as detected by the chemical cross-linker 1,5-difluoro-2,4-dinitrobenzene
30. 2+ as the primary cause for hysteresis in the phosphorylase kinase reactions
31. The isolation and crystallization of rabbit skeletal muscle phosphorylase b
32. Subunit interactions and their relationship to the allosteric properties of rabbit skeletal muscle phosphorylase b
33. Assays of protein kinase
34. Spider and Web: Processing and visualization of images in 3D electron microscopy and related fields