Quantitative gene expression analysis in renal biopsies: A novel protocol for a high-throughput multicenter application

Kidney International

Volumn 61, Issue 1, 2002, Pages 133-140

  Cohen, Clemens D ^a   Frach, Karin ^a   Schlöndorff, Detlef ^a   Kretzler, Matthias ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Lab on a chip; Microdissection; Real time RT PCR; RNA isolation; RNase inhibitor

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; CLINICAL PROTOCOL; CONTROLLED STUDY; ELECTROPHORESIS; GENE EXPRESSION; HUMAN; HUMAN TISSUE; KIDNEY BIOPSY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRESERVATION; PRIORITY JOURNAL; PROTEIN QUALITY; QUALITATIVE ANALYSIS; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0036147231     PISSN: 00852538     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1523-1755.2002.00113.x     Document Type: Article

References (23)

1. Renal biopsy
2. Diagnostic techniques in the work-up of renal allograft dysfunction-an update
3. A reevaluation of routine electron microscopy in the examination of native renal biopsies
4. Future molecular approaches to the diagnosis and treatment of glomerular diseases
5. Molecular biology approach to human kidney biopsies
6. Real-time quantitative RT-PCR after laser-assisted cell picking
7. Chemokine and chemokine receptor expression during initiation and resolution of immune complex glomerulonephritis
8. Analysis of mouse glomerular podocyte mRNA by single-cell reverse transcription-polymerase chain reaction
9. The contribution of increased collagen synthesis to human glomerulosclerosis: A quantitative analysis of alpha 2IV collagen mRNA expression by competitive polymerase chain reaction
10. Molecular analysis of glomerular diseases in renal biopsies: Initial results of a collaborative international study
11. Angiotensin II receptor type 1 gene expression in human glomerulonephritis and diabetes mellitus
12. Glomerular type IV collagen in patients with diabetic nephropathy with and without additional glomerular disease
13. Constitutive expression and modulation of the functional thrombin receptor in the human kidney
14. Renal biopsy collagen I mRNA predicts scarring in rabbit anti-GBM disease: Comparison with conventional measures
15. Approach to the molecular basis of nephron heterogeneity: Application of reverse transcription-polymerase chain reaction to dissected tubule segments
16. A microfluid system for high-speed reproducible DNA sizing and quantitation
17. Processing renal biopsies for diagnostic mRNA quantification: Improvement of RNA extraction and storage conditions
18. Molecular classification of cancer: Class discovery and class prediction by gene expression monitoring
19. Biological stability of RNA isolated from RNAlater-treated brain tumor and neuroblastoma xenografts
20. High yields of RNA and DNA suitable for array analysis from cell sorter purified epithelial cell and tissue populations
21. Gene expression profiles of laser-captured adjacent neuronal subtypes
22. High-fidelity mRNA amplification for gene profiling
23. Laser microdissection and gene expression analysis on formaldehyde-fixed archival tissue