Structure and functional characterization of the periplasmic N-terminal polypeptide domain of the sugar-specific ion channel protein (ScrY porin)

Protein Science

Volumn 11, Issue 6, 2002, Pages 1565-1574

  Michels, Jenny ^a   Geyer, Armin ^a   Mocanu, Viorel ^a   Welte, Wolfram ^a   Burlingame, Alma L ^b   Przybylski, Michael ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Circular dichroism; Coiled coil; Mass spectrometry; N terminal periplasmic domain; ScrY porin; Supramolecular association; TR NOE NMR spectroscopy

Indexed keywords

DIMER; MUTANT PROTEIN; POLYPEPTIDE; PORIN; RECOMBINANT PROTEIN; SUCROSE SPECIFIC PORIN; SUGAR; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; DRUG DERIVATIVE; MALTOSE; MALTOTETROSE; OLIGOSACCHARIDE; PEPTIDE FRAGMENT; SCRY PROTEIN, BACTERIA;

ALPHA HELIX; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BETA SHEET; BINDING AFFINITY; CIRCULAR DICHROISM; CRYSTAL STRUCTURE; ELECTROPHORESIS; LIGHT SCATTERING; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN STRUCTURE; SALMONELLA TYPHIMURIUM; SUPRAMOLECULAR CHEMISTRY; X RAY CRYSTALLOGRAPHY; CHEMISTRY; GENE DELETION; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; PROTEIN TERTIARY STRUCTURE; SPECTROSCOPY;

NEGIBACTERIA; SALMONELLA TYPHIMURIUM;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; MALTOSE; MOLECULAR SEQUENCE DATA; MUTATION; OLIGOSACCHARIDES; PEPTIDE FRAGMENTS; PORINS; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SALMONELLA TYPHIMURIUM; SEQUENCE DELETION; SPECTRUM ANALYSIS;

EID: 0036114292     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.2760102     Document Type: Article

References (29)

1. Mass spectrometric mapping of ion channel proteins (porins) and identification of their supramolecular membrane assembly
2. Determination of the secondary structures of proteins by circular dichroism and optical rotary dispersion
3. The confirmation of the sialyl Lewis X ligand changes upon binding to E-selectin
4. Extended sugar slide function for the periplasmic coiled coil domain of ScrY
5. Direct monitoring of protein-chemical reactions utilizing nanoelectrospray mass spectrometry
6. Crystallisation and preliminary X-ray diffraction analysis of ScrY, a specific bacterial outer membrane porin
7. Structure of the sucrose-specific porin ScrY from Salmonella typhimurium and its complex with sucrose
8. Outer membrane proteins of Pseudomonas
9. Plasmid-mediated sucrose metabolism in Escherichia coll: Characterization of ScrY, the structural gene for a phosphoenolpyruvate-dependent sucrose phosphotransferase system outer membrane porin
10. Synthetic model for two-stranded α-helical coiled coils. Design, synthesis, and characterization of an 86-residue analog of tropomyosin
11. Synthesis of a model protein of defined secondary and quarternary structure. Effect of chain length on the stabilization and formation of two-stranded α-helical coiled-coils
12. Studying noncovalent protein complexes by electrospray ionization mass spectrometry
13. Coiled coils: New structures and new functions
14. Struktur- und funktionscharakterisierung von ionenkanal-proteinen mit proteinchemischen, biophysikalischen und massenspektrometrischen methoden
15. The sucrose-porin (ScrY) from S. thyphimurium: Synthesis, structure characterisation and supramolecular association of the N-terminal polypeptide domain
16. Identification of proteins in polyacrylamide gels by mass spectrometric peptide mapping combined with database search
17. Use of the transferred nuclear Overhauser effect to determine the conformations of ligands bound to proteins
18. Conformation of sLex tetrasaccharide, free in solution and bound to E-P-, and L-Selectin
19. NMR studies of carbohydrateprotein interactions in solution
20. Electrospray mass spectrometry of biomacromolecules complexes with non-covalent interactions - New analytical perspectives for supramolecular chemistry and molecular recognition processes
21. Approaches to the characterization of tertiary and supramolecular protein structures by combination of protein chemistry and mass spectrometry
22. Self-association of a synthetic peptide from the N- terminus of the hepatitis d virus protein into an immunoreactive α-helical multimer
23. Plasmid-mediated uptake and metabolism of sucrose by Escherichia coli K-12
24. A sugar-specific porin, ScrY, is involved in sucrose uptake in enteric bacteria
25. Approaches to the characterization of membrane channel proteins (porins) by UV MALDI-MS
26. The sugar-specific outer membrane channel ScrY contains functional characteristics of general diffusion pores and substrate-specific porins
27. The deletion of 70 amino acids near the N-terminal end of the sucrose-specific porin ScrY causes its functional similarity to LamB in vivo and in vitro
28. Linking genome and proteome by mass spectrometry; large-scale identification of yeast proteins from two dimensional gels
29. Calculation of protein conformation from circular dichroism