Immunoglobulin heavy chain gene analysis in lymphomas: A multi-center study demonstrating the heterogeneity of performance of polymerase chain reaction assays

Journal of Molecular Diagnostics

Volumn 4, Issue 2, 2002, Pages 81-89

  Bagg, Adam ^a   Braziel, Rita M ^b   Arber, Daniel A ^c   Bijwaard, Karen E ^d   Chu, Albert Y ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^c CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

^d ARMED FORCES INSTITUTE OF PATHOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FORMALDEHYDE; IMMUNOGLOBULIN HEAVY CHAIN; PARAFFIN; PARAPROTEIN; PRIMER DNA;

ARTICLE; B CELL LYMPHOMA; CLINICAL LABORATORY; CONTROLLED STUDY; DNA EXTRACTION; DNA FLANKING REGION; FROZEN SECTION; GENE REARRANGEMENT; GENETIC ANALYSIS; HUMAN; HUMAN CELL; HUMAN TISSUE; LABORATORY DIAGNOSIS; METHODOLOGY; MULTICENTER STUDY; PATHOLOGIST; POLYMERASE CHAIN REACTION; PROFESSIONAL PRACTICE; QUESTIONNAIRE; REACTION ANALYSIS; SAMPLE SIZE; SENSITIVITY AND SPECIFICITY; SOUTHERN BLOTTING; STANDARDIZATION; SURGEON; T CELL LYMPHOMA; T LYMPHOCYTE RECEPTOR GENE; TISSUE FIXATION;

EID: 0036100034     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1525-1578(10)60685-X     Document Type: Article

References (47)

1. Molecular pathology of leukemia and lymphoma
2. Minimal residual disease: How low do we go?
3. Analysis of immunoglobulin and T cell receptor genes. Part II: Possibilities and limitations in the diagnosis and management of lymphoproliferative diseases and related disorders
4. The arrangement of immunoglobulin and T cell receptor genes in human lymphoproliferative disorders
5. Application of molecular genetics to the diagnosis of hematopoietic malignancies
6. Molecular diagnostic approach to non-Hodgkin's lymphoma
7. Polymerase chain reaction versus Southern blot hybridization: Detection of immunoglobulin heavy-chain gene rearrangements
8. Physical linkage of a human immunoglobulin heavy chain variable region gene segment to diversity and joining region elements
9. PCR-heteroduplex analysis of T-cell receptor gamma gene rearrangement in paraffin-embedded skin biopsies
10. DNA polymerase chain reaction using fine needle aspiration biopsy smears to evaluate non-Hodgkin's lymphoma
11. Variable rate of detection of immunoglobulin heavy chain V-D-J rearrangement by PCR: A systematic study of 41 B cell non-Hodgkin's lymphomas and leukemias
12. Detection of monoclonality in low-grade B cell lymphomas using the polymerase chain reaction is dependent on primer selection and lymphoma type
13. Evaluation of T cell receptor testing in lymphoid neoplasms: Results of a multi-center study of 29 extracted DNA and paraffin-embedded samples
14. A revised European-American classification of lymphoid neoplasms: A proposal from the International Lymphoma Study Group
15. Development of a highly sensitive assay, based on the polymerase chain reaction, for rare B lymphocyte clones in a polyclonal population
16. Rapid method for distinguishing clonal from polyclonal B cell populations in surgical biopsy specimens
17. Routine application of polymerase chain reaction in the diagnosis of monoclonality of B cell lymphoid proliferations
18. Identification and structural analysis of rearranged immunoglobulin heavy chain genes in lymphomas and leukemias
19. Optimal primer selection for clonality assessment by polymerase chain reaction analysis: I. low grade B cell lymphoproliferative disorders of nonfollicular center cell type
20. Determination of B cell clonality in paraffin-embedded lymph nodes using the polymerase chain reaction
21. Description of a novel FR1 IgH PCR strategy and its comparison with three other strategies for the detection of clonality in B cell malignancies
22. An improved method for detection of B-lymphoid clonality by polymerase chain reaction
23. Monoclonality in B cell lymphoma detected in paraffin wax-embedded sections using the polymerase chain reaction
24. Detection of immunoglobulin gene rearrangement of B cell non-Hodgkin's lymphomas and leukemias in fresh, unfixed and formalin-fixed, paraffin-embedded tissue by polymerase chain reaction
25. Clonality analysis of B cell lymphoma in fresh-frozen and paraffin-embedded tissues: The effects of variable polymerase chain reaction parameters
26. Interfering substances that cause inhibition in polymerase chain reaction (PCR) assays
27. The effect of fixation on detection of B cell clonality by polymerase chain reaction
28. Quality control and sensitivity of polymerase chain reaction techniques for the assessment of immunoglobulin heavy chain gene rearrangements from fixed- and paraffin-embedded samples
29. PCR analysis of immunoglobulin heavy chain (IgH) and TCR-γ chain gene rearrangements in the diagnosis of lymphoproliferative disorders: Results of a study of 525 cases
30. Comparison of different polymerase chain reaction-based approaches for clonality assessment of immunoglobulin heavy-chain gene rearrangements in B cell neoplasia
31. Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections
32. Optimal primer selection for clonality assessment by polymerase chain reaction analysis: III. intermediate and high-grade B cell neoplasms
33. Detection of clonal B cell populations in paraffin-embedded tissues by polymerase chain reaction
34. Detection of immunoglobulin heavy chain genes rearrangements in B cell leukemias, lymphomas, multiple myelomas, monoclonal, and polyclonal gammopathies
35. Detection of immunoglobulin kappa light chain rearrangements by polymerase chain reaction: An improved method for detecting clonal B cell lymphoproliferative disorders
36. Improved PCR method for detecting monoclonal immunoglobulin heavy chain rearrangement in B cell neoplasms
37. Lymphoid tumors displaying rearrangements of both immunoglobulin and T cell receptor genes
38. B cell target DNA quantity is a critical factor in the interpretation of B cell clonality by PCR
39. PCR analysis of the immunoglobulin heavy chain gene in polyclonal processes can yield pseudoclonal bands as an artifact of low B cell number
40. Limitations of clonality analysis of B cell proliferations using CDR3 polymerase chain reaction
41. Variability of polymerase chain reaction detection of the bcl-2-lgH translocation in an international multi-centre study
42. Quality assessment in diagnostic molecular pathology: Experience from a German-Austrian-Swiss multi-center trial
43. Quality assurance in RT-PCR-based BCR/ABL diagnostics: Results of an inter-laboratory test and a standardization approach
44. Variability in the levels of PML-RARa fusion transcripts detected by the laboratories participating in an external quality control program using several reverse transcription polymerase chain reaction protocols
45. Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. Report of the BIOMED-1 Concerted Action: Investigation of minimal acute leukemia