A CLN2-related and thermostable serine-carboxyl proteinase, kumamolysin: Cloning, expression, and identification of catalytic serine residue

Journal of Biochemistry

Volumn 131, Issue 5, 2002, Pages 757-765

  Oyama, Hiroshi ^a   Hamada, Takatoshi ^b   Ogasawara, Shin ^a   Uchida, Kenichi ^c   Murao, Sawao ^b   Beyer, Bret B ^d   Dunn, Ben M ^d   Oda, Kohei ^a  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^b SOJO UNIVERSITY   (Japan)

^c TEIKYO UNIVERSITY   (Japan)

^d UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

Author keywords

Catalytic triad; Kumamolysin; Pepstatin insensitive carboxyl proteinase; Serine carboxyl proteinase; Thermostable proteinase

Indexed keywords

ASPARTIC PROTEINASE; KUMAMOLYSIN; PROTEINASE INHIBITOR; RECOMBINANT PROTEIN; SERINE; AMINO ACID; ASPARTIC ACID; CARBOHYDRATE DERIVATIVE; GLUTAMIC ACID; PEPSTATIN; PROTEINASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; DRUG ANTAGONISM; ENZYME ACTIVE SITE; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HEAT; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; RESTRICTION MAPPING; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; WESTERN BLOTTING; AMINO TERMINAL SEQUENCE; CATALYSIS; DEGENERATIVE DISEASE; ENZYME ACTIVITY; ENZYME INACTIVATION; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; PSEUDOMONAS; THERMOSTABILITY; XANTHOMONAS;

AMINO ACID SEQUENCE; ASPARTIC ENDOPEPTIDASES; BASE SEQUENCE; BLOTTING, WESTERN; CATALYTIC DOMAIN; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; HEAT; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEASE INHIBITORS; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SEQUENCE HOMOLOGY; SERINE; STRUCTURE-ACTIVITY RELATIONSHIP;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; XANTHOMONAS;

EID: 0036090236     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003162     Document Type: Article

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