Purification and properties of monomeric (G1) forms of acetylcholinesterase secreted by Nippostrongylus brasiliensis

Molecular and Biochemical Parasitology

Volumn 90, Issue 2, 1997, Pages 513-524

  Grigg, Michael E ^a   Tang, Liang ^a   Hussein, Ayman S ^a   Selkirk, Murray E ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Acetylcholinesterase; Nippostrongylus brasiliensis

Indexed keywords

1,5 BIS(4 ALLYLDIMETHYLAMMONIUMPHENYL)PENTAN 3 ONE DIBROMIDE; ACETYLCHOLINESTERASE; CHOLINESTERASE INHIBITOR; DETERGENT; GALLAMINE; ISO OMPA; MONOMER; PHYSOSTIGMINE; PROPIDIUM IODIDE; SEPHAROSE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SUBSTRATE; GEL PERMEATION CHROMATOGRAPHY; MOLECULAR WEIGHT; NIPPOSTRONGYLUS BRASILIENSIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SUCROSE DENSITY GRADIENT CENTRIFUGATION;

ACETYLCHOLINESTERASE; ACETYLTHIOCHOLINE; ANIMALS; CENTRIFUGATION, DENSITY GRADIENT; CHOLINESTERASE INHIBITORS; CHROMATOGRAPHY, GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; NIPPOSTRONGYLUS; SUBSTRATE SPECIFICITY; TETRAISOPROPYLPYROPHOSPHAMIDE;

INVERTEBRATA; NEMATODA; NIPPOSTRONGYLUS BRASILIENSIS;

EID: 0031574297     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(97)00202-8     Document Type: Article

References (40)

1. Taylor P The cholinesterases. J Biol Chem. 266:1991;4025-4028.
2. Massoulié J, Sussman JL, Bon S, Silman I Structure and functions of acetylcholinesterase and butyrylcholinesterase. Prog Brain Res. 98:1993;139-146.
3. Inestrosa NC, Roberts WL, Marshall TL, Rosenberry TL Acetylcholinesterase from bovine caudate nucleus is attached to membranes by a novel subunit distinct from those of acetylcholinesterase in other tissues. J Biol Chem. 262:1987;4441-4444.
4. Silman I, Futerman AH Modes of attachment of acetylcholinesterase to the surface membrane. Eur J Biochem. 170:1987;11-22.
5. Roberts WL, Kim BH, Rosenberry TL Differences in the glycolipid membrane anchor of bovine and human erythrocyte acetylcholinesterases. Proc Natl Acad Sci USA. 84:1987;7817-7821.
6. Bon S, Toutant JP, Méflah K, Massoulié J Amphiphilic and nonamphiphilic forms of Torpedo cholinesterases: II. Electrophoretic variants and phosphatidylinositol phospholipase C-sensitive and -insensitive forms. J Neurochem. 51:1988;786-794.
7. Johnson D, Russell RL Multiple forms of acetylcholinesterase in the nematode Caenorhabditis elegans. J Neurochem. 41:1983;30-46.
8. Toutant J-P, Arpagaus M, Fournier D Native molecular forms of head acetylcholinesterase from adult Drosophila melanogaster: quaternary structure and hydrophobic character. J Neurochem. 50:1988;209-218.
9. Fournier D, Bergé J-B, Cardoso de Almeida DL, Bordier C Acetylcholinesterase from Musca domestica and Drosophila melanogaster brain are linked to membranes by a glycophospholipid anchor sensitive to an endogenous phospholipase. J Neurochem. 50:1988;1158-1163.
10. Arpagaus M, Richier P, Berge J-B, Toutant J-P Acetylcholinesterases of the nematode Steinernema carpocapsae. Characterization of two types of amphiphilic forms differering in their mode of membrane association. Eur J Biochem. 207:1992;1101-1108.
11. Johnson CD, Stretton AOW. Neural control of locomotion in Ascaris: anatomy, electrophysiology and biochemistry. In: Zuckerman B, editor. Nematodes as Biological Models. London: Academic Press, 1980:159-95.
12. Chalfie M, White J. The Nervous System. In: Wood WB, editor. The Nematode Caenorhabditis elegans. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1988:337-91.
13. Johnson CD, Duckett JG, Culotti JG, Herman PM, Meneely PM, Russell RL An acetylcholinesterase-deficient mutant of the nematode Cacnorhabditis elegans. Genetics. 97:1981;261-279.
14. Culotti JG, Von Ehrenstein G, Culotti M, Russell RL A second class of acetylcholinesterase-deficient mutants of the nematode Caenorhabditis elegans. Genetics. 97:1981;281-305.
15. Johnson CD, Rand JR, Herman RK, Stern BD, Russell RL The acetylcholinesterase genes of C. elegans: identification of a third gene (ace-3) and mosaic mapping of a synthetic lethal phenotype. Neuron. 1:1988;165-173.
16. Arpagaus M, Fedon Y, Cousin X, Chatonnet A, Bergé J-B, Fournier D, Toutant J-P cDNA sequence, gene structure, and in vitro expression of ace-l, the gene encoding acetylcholinesterase of class A in the nematode Caenorhabditis elegans. J Biol Chem. 269:1994;9957-9965.
17. Sanderson BE Release of cholinesterase by adult Nippostrongylus brasiliensis in vitro. Z Parasite. 40:1972;1-7.
18. Ogilvie BM, Rothwell TLW, Bremner KC, Schitzerling HJ, Nolan J, Keith RK Acetylcholinesterase secretion by parasitic nematodes, 1. Evidence for secretion of the enzyme by a number of species. Int J Parasitol. 3:1973;589-597.
19. Rhoads ML Cholinesterase in the parasitic nematode Stephanurus dentatus. Characterisation and sex dependence of a secretory cholinesterase. J Biol Chem. 256:1981;9316-9323.
20. Lee DL The fine structure of the excretory system in adult Nippostrongylus brasiliensis (Nematoda) and a suggested function for the enzyme. Tissue Cell. 2:1970;225-231.
21. McLaren DJ, Burt JS, Ogilvie BM The anterior glands of adult Necator americanus (nematode: Strongyloidea)-II. Cytochemical and functional studies. Int J Parasitol. 4:1974;39-46.
22. Pritchard DI, Leggett KV, Rogan MT, McKean PG, Brown A Necator americanus secretory acetylcholinesterase and its purification from excretory-secretory products by affinity chromatography. Parasite Immunol. 113:1991;187-199.
23. Pritchard DI, Brown A, Toutant J-P The molecular forms of acetylcholinesterase from Necator americanus (Nematoda), a hookworm parasite of the human intestine. Eur J Biochem. 219:1994;317-323.
24. Griffiths G, Pritchard DI Purification and biochemical characterisation of acetylcholinesterase (ACHE) from the excretory/secretory products of Trichostrongylus colubriformis. Parasitology. 108:1994;579-586.
25. Rhoads ML Secretory cholinesterases of nematodes: possible functions in the host-parasite relationship. Trop Vet. 2:1984;3-10.
26. Philipp MT Acetylcholinesterase secreted by intestinal nematodes: a reinterpretation of its putative role of 'biochemical holdfast'. Trans R Soc Trop Med Hyg. 78:1984;138-139.
27. Edwards AJ, Burt JS, Ogilvie BM The effect of host immunity upon some enzymes of the parasitic nematode Nippostrongylus brasiliensis. Parasitology. 62:1971;339-347.
28. Sanderson BE, Jenkins DC, Ogilvie BM Nippostrongylus brasiliensis: relation between immune damage and acetylcholinesterase levels. Int J Parasitol. 2:1972;227-232.
29. Sanderson BE, Jenkins DC, Phillipson RF Nippostrongylus brasiliensis: further studies of the relation between host immunity and worm acetylcholinesterase levels. Int J Parasitol. 6:1976;99-102.
30. Blackburn CC, Selkirk ME Characterisation of the secretory acetylcholinesterases from adult Nippostrongylus brasiliensis. Mol Biochem Parasitol. 53:1992;79-88.
31. Sine J-P, Toutant J-P, Weigel P, Colas B Amphiphilic forms of butyrylcholinesterase in mucosal cells of rat intestine. Biochemistry. 31:1992;10893-10900.
32. Dudai Y, Silman I Acetylcholinesterase. Methods Enzymol. 34:1974;571-580.
33. Karnovsky MJ, Roots L A 'direct-coloring' method for cholinesterases. J Histochem Cytochem. 12:1964;219-221.
34. Ellman GL, Courtney KD, Andres V, Featherstone RM A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 7:1961;88-95.
35. Austin L, Berry WK Two selective inhibitors of cholinesterase. Biochem J. 54:1953;695-700.
36. Healer J, Ashall F, Maizels RM Characterization of proteolytic enzymes from larval and adult Nippostrongylus brasiliensis. Parasitology. 103:1991;305-314.
37. Radic Z, Reiner E, Taylor P Role of the peripheral anionic site on acetylcholinesterase: inhibition by substrates and coumarin derivatives. Mol Pharmacol. 39:1991;98-104.
38. Radic Z, Pickering NA, Vellom DC, Camp S, Taylor P Three distinct domains in the cholinesterase molecule confer selectivity for acetyl- and butyryl-cholinesterase inhibitors. Biochemistry. 32:1993;12074-12084.
39. Eichler J, Anselmet A, Sussman JL, Massoulié J, Silman I Differential effects of 'peripheral' site ligands on Torpedo and chicken acetylcholinesterase. Mol Pharmacol. 45:1994;335-340.
40. Radic Z, Gibney G, Kawamoto S, MacPhee-Quigley K, Bongiorno C, Taylor P Expression of recombinant acetylcholinesterase in a baculovirus system: kinetic properties of glutamate 199 mutants. Biochemistry. 31:1992;9760-9767.