Evaluation of PCR assays in presence of antibody to thermostable DNA polymerases for detection of microbial agents: Avoiding false negative results for specimen containing low-titer agent

Journal of Clinical Laboratory Analysis

Volumn 12, Issue 4, 1998, Pages 238-241

  Poddar, Saibal K ^a   Sawyer, Mark H ^a   Connor, James D ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

Monoclonal antibody; Taq DNA polymerase; Tth DNA polymerase

Indexed keywords

DNA POLYMERASE; MONOCLONAL ANTIBODY;

ADENOVIRUS; ANTIBODY TITER; ARTICLE; BACTERIUM DETECTION; CONTAMINATION; DNA SYNTHESIS INHIBITION; ENZYME ASSAY; GENE TARGETING; INFLUENZA VIRUS; NONHUMAN; TARGET CELL; THERMOSTABILITY; VIRUS DETECTION;

ADENOVIRUSES, HUMAN; ANTIBODIES, MONOCLONAL; DNA, VIRAL; DNA-DIRECTED DNA POLYMERASE; FALSE POSITIVE REACTIONS; HUMANS; INFLUENZA A VIRUS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TAQ POLYMERASE;

ADENO VIRUS; ADENOVIRIDAE; BACTERIA (MICROORGANISMS); INFLUENZA A VIRUS; INFLUENZA VIRUS;

EID: 0031746780     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2825(1998)12:4<238::AID-JCLA8>3.0.CO;2-3     Document Type: Article

References (17)

1. Innis MA, Gelfand DH: Optimization of PCR. In PCR Protocols: A Guide io Methods and Applications. M.A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White, eds. Academic Press, Inc., New York, 1990.
2. Pring-Akerblom P, Adrian T: Type and group specific polymerase chain reaction for adenovirus detection. Res Virol 145:25-35, 1994.
3. Wright KE., Wilson GAR, Novosad D, Dimock C, Tan D, Weber JM: Typing and subtyping of influenza viruses in clinical samples by PCR. J Clin Microbiol 33:1180-1184, 1995.
4. Fujimura RK, Shapshak P, Feaster D, Epler M, Goodkin K: A rapid method for comparative quantitative polymerase chain reaction of HIV-1 proviral DNA extracted from cryopreserved brain tissues. J Virol Methods 67:177-187 1997.
5. Mulder J, Mckinney N, Christopherson C, Sninnsky J, Greenfield L, Kwok S: Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma: Application to acute infection. J Clin Microbiol 32:292-300, 1994.
6. Bovin G, Gilbert C, Morissette M, Handfield J, Goyette N, Bergeron MG: A case of ganciclovir-resistant cytomegalovirus (CMV) retinitis in a patient with AIDS: Longitudinal molecular analysis of the CMV viral load and viral mutations in blood compartments. AIDS 11:867-873, 1997.
7. Kwok S, Higuchi R: Avoiding false positives with PCR. Nature 339:237-238, 1989.
8. Richard WC, Hobson AC, Huang MW: Coamplified positive control detects inhibition of polymerase chain reaction. J Clin Microbiol 30:3185-3189, 1992.
9. Wadowski RM, Laus S, Libert T, States SJ, Ehrlich GD: Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab. J Clin Microbiol 32:1054-1057, 1994.
10. Wiedbrauk DL, Werner JC, Drevon AM: Inhibition of PCR by aqueous and vitreous fluids. J Clin Microbiol 33:2643-2646, 1995.
11. Poddar SK, Sawyer MH, Connor JD: Effect of inhibitors in clinical specimens on Taq and Tth DNA plymerase-based PCR amplification of Influenza A virus. J Med Microbiol. (in press), 1998.
12. D'Aquila RT, Bechtel LJ, Videler JA, Eron JJ, Gorczyca P, Kaplan JC: Maximimizing sensitivity and specificity of PCR by preamplification heating. Nucleic Acids Res 20:1717-1723, 1991.
13. Kellogg DE, Rybalkin I, Chen S, et al.: Taqstart antibody: Hot start PCR facilitated by neutralizing monoclonal antibody directed against Taq DNA polymerase. BioTechniques 16:1134-1137, 1994.
14. Poddar SK, Sawyer MH, Connor JD: Optimized PCR amplification of influenza A virus RNA using Tth DNA polymerase, incorporating uracil N glycosylase (UNG) in a single tube reaction: J Clin Lab Anal 11:323-327, 1997
15. Kinchington PR, Turse SE, Kowalski RP, Gordon YJ: Use of polymerase chain reaction for the detection of adenoviruses in ocular swab specimens. Invest Ophthalmol Vis Sci 35:4126-4134, 1994.
16. Cheng J, Shoffner MA, Hvichia GE, Kricka LJ, Wilding P: Chip PCR II: Investigation of different PCR amplification systems in microfabricated silicon-glass chips. Nucleic Acids Res 24:380-385, 1996.
17. Jayasena, SD: Oligonucleotide aptamers to replace manual "Hot-start" conditions in PCR. Abstract in Cambridge Healthtech Institute's fifth annual conference on advances in nucleic acid amplification and detection, 1997.