Routine preparation of air-dried negatively stained and unstained specimens on holey carbon support films: A review of applications

Micron

Volumn 33, Issue 5, 2002, Pages 461-480

  Harris, J Robin ^a   Scheffler, Dirk ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Ammonium molybdate; Holey carbon films; Negative staining; Transmission electron microscopy (TEM); Trehalose

Indexed keywords

PHASEOLUS (ANGIOSPERM);

CARBON; HEMOCYANIN; KEYHOLE LIMPET HEMOCYANIN; KEYHOLE-LIMPET HEMOCYANIN; LIPID; LIPOPROTEIN; MACROGOL DERIVATIVE; POLYMER; PROTEIN; TREHALOSE;

ANIMAL; ARTICLE; CELL ORGANELLE; CHEMISTRY; ELECTRON MICROSCOPY; HISTOLOGY; HUMAN; METHODOLOGY; STAINING; ULTRASTRUCTURE; VIRUS;

ANIMALS; CARBON; HEMOCYANIN; HISTOLOGICAL TECHNIQUES; HUMANS; LIPIDS; LIPOPROTEINS; MICROSCOPY, ELECTRON; ORGANELLES; POLYETHYLENE GLYCOLS; POLYMERS; PROTEINS; STAINING AND LABELING; TREHALOSE; VIRUSES;

EID: 0036013787     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-4328(01)00039-7     Document Type: Article

References (78)

1. Iron-dependent self-assembly of recombinant yeast frataxin: Implications for Friedreich ataxia
2. Cryoelectron microscopy of viruses
3. Cryo-negative staining
4. Cryoelectron microscopy of protein-lipid complexes of human myelin basic protein charge isomer differing in degree of citrullination
5. Functional ABC triblock copolymers for controlled surface patterns on nanometer scale
6. A negative staining method for high resolution electron microscopy of viruses
7. The molecular architecture of the extracellular hemoglobin of Ophelia bicornis; Analysis of individual molecules
8. The structure of ferritin cores determined by electron nanodiffraction
9. Preservation of mammalian cells - Learning nature's tricks
10. Particle-surface interaction in thin vitrified films for cryo-electron microscopy
11. Unexpected property of trehalose as observed by cryo-electron microscopy
12. Intracellular trehalose improves the survival of cryopreserved mammalian cells
13. Electron microscopy of a hemagglutinin from Limulus polyphemus
14. Macromolecular organization of hemocyanins and apohemocyanins as revealed by electron microscopy
15. Controlled cleavage of KLH1 and KLH2 by the V8 protease from Staphylococcus aureus
16. The molecular architecture of the extracellular hemoglobin of Ophelia bicornis
17. Limitations to significant information in biological electron microscopy as a result of radiation damage
18. The production of paracrystalline two-dimensional monolayers of purified protein molecules
19. A negative staining study of aqueous suspensions of sphingomyelin
20. Electron microscopy of cholesterol
21. The negative staining carbon film procedure: Technical considerations and a survey of macromolecular applications
22. Negative staining and cryoelectron microscopy: The thin film techniques
23. Negative staining of thinly spread biological particulates
24. Preparation of thin-film frozen-hydrated/vitrified biological specimens for cryolelectron microscopy
25. A negative staining study of human erythrocyte ghosts and rat liver nuclear membranes
26. Human erythrocyte catalase: 2-D crystal nucleation and multiple 2-D crystal forms
27. Negative staining
28. Negative staining: A brief assessment of current technical benefits, limitations and future possibilities
29. Two-dimensional crystallization, transmission electron microscopy and image processing of keyhole limpet hemocyanin (KLH)
30. Electron microscopy of a double helical tubular filament in keyhole limpet (Megathura crenulata) hemolymph
31. Human erythrocyte catalase: New 2-dimensional crystal forms and image processing
32. Keyhole limpet hemocyanin (KLH): Negative staining in the presence of trehalose
33. Keyhole limpet hemocyanin (KLH): Purification of intact KLH1 through selective dissociation of KLH2
34. The structure of gas-filled n-butyl-2-cyanoacrylate (BCA) polymer particles
35. Negative stain containing trehalose: Applications to tubular and filamentous structure
36. Keyhole limpet hemocyanin (KLH). I: Reassociation from Immucothel® followed by separation of KLH1 and KLH2
37. Keyhole limpet hemocyanin (KLH). II: Characteristic reassociation properties of purified KLH1 and KLH2
38. Keyhole limpet hemocyanin (KLH): Slow in vitro reassociation of KLH1 and KLH2
39. Cholesterol-steptolysin O interaction: An EM study of wild-type and mutant streptolysin O
40. Application of the negative staining technique to both aqueous and organic solvent solutions of polymer particles
41. Haliotis tuberculata hemocyanin (HtH): Analysis of oligomeric stability of HtH1 and HtH2, and comparison with keyhole limpet hemocyanin KLH1 and KLH2
42. Comparison of the decameric structure of peroxiredoxin II by transmission electron microscopy and X-ray crystallography
43. Transmission electron microscopical studies on some hemolymph proteins from the marine polychaete Nereis virens
44. Trehalose embedding technique for high-resolution electron crystallography: Application to structural study on bacteriorhodopsin
45. A negative staining-carbon film technique for studying viruses in the electron microscope. I. Preparation procedures for examining icosahedral and filamentous viruses
46. Electron microscope observations on the structure of microsomal particles from Escherichia Coli
47. Molecular design of PhoE porin and its functional consequences
48. Structure of PhoE porin in projection at 3.5 Å resolution
49. Structural architecture of an outer membrane channel as determined by electron crystallography
50. 20-channel AQP-CHIP, in projection at 3.5 Å resolution
51. Negative staining of proteins
52. Utility of butvar support film and methylamine tungstate stain in three-dimensional electron microscopy: Agreement between stain and frozen-hydrated reconstructions
53. Two-dimensional crystallization of membrane proteins
54. Lowresolution data analysis for low-density lipoprotein particle
55. Negative staining of protein macromolecules: A simple rapid method
56. Double-shell vesicles, strings of vesicles and filaments found in crosslinked micellar solutions of poly(1,2-butadiene)-block-poly(ethylene oxide) diblock copolymers
57. Ultrastructure of vitrified (frozen-hydrated) ferritin and apoferritin
58. Ultrastructure of ferritin and apoferritin
59. Unconventional negative staining: Heavy metals are not required for negative staining
60. Light atom derivatives of structure-preserving sugars are unconventional negative stains
61. Quaternary structure of gastropod hemocyanin
62. The fibril structure of type V collagen triple-helical domain
63. Evidence for conformational stabilization of beta-lactoglobulin when dried with trehalose
64. Cryotechniques in macromolecular research
65. Structure of keyhole limpet hemocyanin type 1 (KLH1) at 15 Å resolution by electron cryomicroscopy and angular reconstitution
66. Three-dimensional structure of low density lipoproteins by electron cryomicroscopy
67. Ultrastructure, fractionation and biochemical analysis of Cryptosporidium parvum sporozoites
68. Nanoparticles built of cross-linked heterotelechelic, amphiphilic poly(dimethylsiloxane)-b-poly(ethylene oxide) diblock copolymers
69. +-ATPase from Kluyveromyces lactis. Protection by trehalose
70. Wormlike polystyrene brushes in thin films
71. Spreading of carbosilane dendrimers at the air/water interface
72. On the negative staining of the protein crystal structure
73. Capsid fine structure of cowpea chlorotic mottle virus: From a computer analysis of negatively stained virus arrays
74. Comparison of the low-resolution structures of ornithine decarboxylase by electron microscopy and X-ray crystallography: The utility of methylamine tungstate and Butvar support film in the study of macromolecules by transmission electron microscopy
75. Beef liver catalase structure: Interpretation of electron micrographs
76. Molecular structure determination by electron microscopy of unstained acrystalline specimens
77. The material bone: Structure-mechanical function relations
78. The formation of two-dimensional arrays of isometric plant viruses in the presence of polyethylene glycol