Utility of MTT assay in three-dimensional cultured human skin model as an alternative for Draize skin irritation test: Approach using diffusion law of irritant in skin and toxicokinetics-toxicodynamics correlation

Pharmaceutical Research

Volumn 19, Issue 5, 2002, Pages 669-675

  Watanabe, T ^a   Hasegawa, T ^a   Takahashi, H ^b   Ishibashi, T ^b   Itagaki, H ^c   Sugibayashi, K ^a  

^a JOSAI UNIVERSITY   (Japan)

^b TOYOBO CO LTD   (Japan)

^c SHISEIDO RESEARCH CENTER   (Japan)

Author keywords

Cetylpyridinium chloride; Draize score; Living Skin Equivalent high; MTT assay; Skin irritation

Indexed keywords

CETYLPYRIDINIUM SALT; IRRITANT AGENT; SURFACTANT; TETRAZOLIUM; THIAZOYL BLUE; UNCLASSIFIED DRUG; COLORING AGENT; THIAZOLE DERIVATIVE; THIAZOLYL BLUE;

ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL COUNT; CELL VIABILITY; COLORIMETRY; CONTROLLED STUDY; CYTOTOXICITY; DISEASE MODEL; DRAIZE SKIN IRRITATION TEST; GUINEA PIG; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IC 50; IN VITRO STUDY; IN VIVO STUDY; MALE; NONHUMAN; PRIORITY JOURNAL; SCORING SYSTEM; SKIN CULTURE; SKIN PERMEABILITY; TOXICOKINETICS; ANIMAL; BIOLOGICAL MODEL; COMPARATIVE STUDY; DRUG EFFECT; HUMAN; METHODOLOGY; PREDICTION AND FORECASTING; SKIN; SKIN TEST; TOXICITY TESTING;

ANIMAL; CETYLPYRIDINIUM; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COLORIMETRY; COMPARATIVE STUDY; DYES; GUINEA PIGS; HUMAN; IN VITRO; IRRITANTS; MALE; MODELS, BIOLOGICAL; PREDICTIVE VALUE OF TESTS; SKIN; SKIN TESTS; TETRAZOLIUM SALTS; THIAZOLES; TOXICITY TESTS; ANIMALS; COLORING AGENTS; HUMANS;

EID: 0036001404     PISSN: 07248741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1015366331634     Document Type: Article

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