Repression and activation domains of Rme1p structurally overlap, but differ in genetic requirements

Molecular Biology of the Cell

Volumn 13, Issue 5, 2002, Pages 1709-1721

  Blumental Perry, Anna ^a   Li, Weishi ^a   Simchen, Giora ^a   Mitchell, Aaron P ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; PROTEIN RME1P; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN; FUNGAL PROTEIN; REPRESSOR PROTEIN; RGR1 PROTEIN, S CEREVISIAE; RME1 PROTEIN, S CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSCRIPTION FACTOR;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; GENE MUTATION; MEIOSIS; PRIORITY JOURNAL; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; TRANSCRIPTION REGULATION; BINDING SITE; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BINDING SITES; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; MEIOSIS; PROTEIN STRUCTURE, TERTIARY; REPRESSOR PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION, GENETIC; ZINC FINGERS; TRANSCRIPTION FACTORS;

SACCHAROMYCES CEREVISIAE;

EID: 0036000017     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.01-09-0468     Document Type: Article

References (45)

1. Mutational analysis of the transcription activation domain of Re1A: Identification of a highly synergistic minimal acidic activation module
2. Positive control of yeast meiotic genes by the negative regulator UME6
3. A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast gene
4. Characterization of functional domains within the multifunctional transcription factor,YY1
5. Genetics of transcriptional regulation in yeast: Connections to the RNA polymerase II CTD
6. The yeast RME1 gene encodes a putative zinc finger protein that is directly repressed by a1-a2
7. Repression by the yeast meiotic inhibitor RME1
8. Requirement for RGR1 and SIN4 in RME1-dependent repression in Saccharomyces cerevisiae
9. The transcriptional activator GCN4 contains multiple activation domains that are critically dependent on hydrophobic amino acids
10. Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site
11. The Epstein-Barr virus R transactivator (Rta) contains a complex, potent activation domain with properties different from those of VP16
12. Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein
13. Identification of seven hydrophobic clusters in GCN4 making redundant contributions to transcriptional activation
14. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast
15. Genetic and physical interactions between yeast RGR1 and SIN4 in chromatin organization and transcriptional regulation
16. Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae
17. Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters
18. Carbohydrate metabolism during ascospore development in yeast
19. IME1, a positive regulator gene of meiosis in S. cerevisiae
20. Regulation of mating and meiosis in yeast by the mating-type region
21. Meiosis and sporulation in Saccharomyces cerevisiae
22. Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme
23. Interaction of yeast repressor-activator protein Ume6p with glycogen synthase kinase 3 homolog Rim11p
24. Activation of meiosis and sporulation by repression of the RME1 product in yeast
25. Mediator protein mutations that selectively abolish activated transcription
26. The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression
27. Pattern of aromatic and hydrophobic amino acids critical for one of two subdomains of the VP16 transcriptional activator
28. Induction of meiosis in Saccharomyces cerevisiae depends on conversion of the transcriptional repressor Ume6 to a positive regulator by its regulated association with the transcriptional activator Ime1
29. Generating yeast transcriptional activators containing no yeast protein sequences
30. Structure and molecular analysis of RGR1, a gene required for glucose repression of Saccharomyces cerevisiae
31. Dissection of the DNA binding domain of yeast Zn-finger protein Rme1p, a repressor of meiotic activator IME1
32. Genomic footprinting of the yeast zinc finger protein Rme1p and its roles in repression of the meiotic activator IME1
33. Transcriptional repression at a distance through exclusion of activator binding in vivo
34. A C-terminal segment with properties of α-helix is essential for DNA binding, and in vivo function of zinc finger protein Rme1p
35. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
36. Role of IME1 expression in regulation of meiosis in Saccharomyces cerevisiae
37. Epistasis analysis of suppressor mutations that allow HO expression in the absence of the yeast SW15 transcriptional activator
38. Identification of functionally related genes that stimulate early meiotic gene expression in yeast
39. Separation of transcriptional activation and silencing functions of the RAP1-encoded repressor/ activator protein 1: Isolation of viable mutants affecting both silencing and telomere length
40. Rme1, a negative regulator of meiosis, is also a positive activator of G1 cyclin gene expression
41. Stimulation of yeast meiotic gene expression by the glucose-repressible protein kinase Riml5p
42. Transcriptional activation by the acidic domain of Vmw65 requires the integrity of the domain and involves additional determinants distinct from those necessary for TFIIB binding