Conformational changes at the active site of adenylate kinase detected using a fluorescent probe and monoclonal antibody binding

Biochimie

Volumn 84, Issue 4, 2002, Pages 335-339

  Zhang, Tian Hong ^a   Luo, Jie ^a   Zhou, Jun Mei ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

Author keywords

Adenylate kinase; Conformational change; Fluorescence quenching; Fluorescent probe; Monoclonal antibody

Indexed keywords

ADENYLATE KINASE; GUANIDINE; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 3D3; UNCLASSIFIED DRUG; CYSTEINE; FLUORESCENT DYE; UREA;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; BINDING KINETICS; CONCENTRATION RESPONSE; CONFORMATIONAL TRANSITION; DRUG BINDING; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME DENATURATION; FLUORESCENCE ANALYSIS; MOLECULAR PROBE; NONHUMAN; PROTEIN FOLDING; RABBIT; ANIMAL; BINDING SITE; CHEMISTRY; FLUORESCENCE; METABOLISM; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DENATURATION; TIME;

ADENYLATE KINASE; ANIMAL; ANTIBODIES, MONOCLONAL; BINDING SITES; CYSTEINE; FLUORESCENCE; FLUORESCENT DYES; GUANIDINE; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DENATURATION; RABBITS; SUPPORT, NON-U.S. GOV'T; TIME FACTORS; UREA; ANIMALS;

EID: 0035997036     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-9084(02)01384-6     Document Type: Article

References (18)

1. Studies on adenosine triphosphate transphorylases, amino acid sequence of rabbit muscle ATP-AMP transphosphorylase
2. Studies on adenosine triphosphate transphosphorylases XIV, equilibrium binding properties of the crystalline rabbit and calf muscle ATP-AMP transphosphorylase (adenylate kinase) and derived peptide fragments
3. Inactivation precedes conformation change during thermal denaturation of adenylate kinase
4. Sequential unfolding of adenylate kinase during denaturation by guanidine hydrochloride
5. Refolding of urea-denaturated adenylate kinase
6. Activation and conformational changes of adenylate kinase in urea solution
7. Preparation and characterization of monoclonal antibodies against adenylate kinase
8. Use of monoclonal antibodies in the study of conformation and conformational alterations in proteins
9. Investigating protein conformation, dynamics and folding with monoclonal antibodies
10. Energy transfer in lactose repressor protein modified with N-{[(iodoacetyl)amino]ethyl}-5-naphthylamine-1-sulfonate
11. Synthesis and characterization of two fluorescent sulfhydryl reagents
12. Fluorescent and spin label probes of the environments of the sulfhydryl groups of porcine muscle adenylate kinase
13. Refined structure of porcine cytosolic adenylate kinase at 2.1 Å resolution
14. Activation of adenylate kinase by denaturants is due to the increasing conformational flexibility at its active sites
15. Location of the active sites of some enzymes in limited and flexible molecular regions
16. Conformational flexibility of enzyme active sites
17. Inactivation precedes overall molecular conformation changes during enzyme denaturation