The use of nested polymerase chain reaction and restriction fragment length polymorphism for the detection and typing of mucosal human papillomaviruses in samples containing low copy numbers of viral DNA

Journal of Virological Methods

Volumn 105, Issue 1, 2002, Pages 159-170

  Kay, Patti ^a   Meehan, Kathleen ^b   Williamson, Anna Lise ^a,c  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

^b CAPE PENINSULA UNIVERSITY OF TECHNOLOGY   (South Africa)

^c UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Low copy numbers; Nested PCR; Novel; RFLP

Indexed keywords

PRIMER DNA; VIRUS DNA;

ARTICLE; CHEEK MUCOSA; CLINICAL ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; DNA SEQUENCE; FEMALE; GENBANK; HUMAN; HUMAN CELL; IMMUNE DEFICIENCY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; VIRUS DETECTION; VIRUS DIAGNOSIS; VIRUS IDENTIFICATION; VIRUS INFECTION; VIRUS ISOLATION; VIRUS TYPING; WART VIRUS; CLASSIFICATION; EVALUATION; GENE DOSAGE; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MOUTH MUCOSA; OROPHARYNX TUMOR; PAPILLOMA VIRUS; UTERINE CERVIX CARCINOMA IN SITU; VIROLOGY;

CERVICAL INTRAEPITHELIAL NEOPLASIA; DNA, VIRAL; FEMALE; GENE DOSAGE; HUMAN; MOUTH MUCOSA; OROPHARYNGEAL NEOPLASMS; PAPILLOMAVIRUS, HUMAN; PAPOVAVIRIDAE INFECTIONS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SUPPORT, NON-U.S. GOV'T; TUMOR VIRUS INFECTIONS; HUMANS; PAPILLOMAVIRIDAE; PAPILLOMAVIRUS INFECTIONS;

HUMAN PAPILLOMAVIRUS TYPE 16; PAPILLOMAVIRUS;

EID: 0035994784     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(02)00100-3     Document Type: Article

References (32)

1. Concurrent HPV infection in oral and genital mucosa
2. Identification and assessment of known and novel human papillomaviruses by polymerase chain reaction amplification, restriction fragment length polymorphisms, nucleotide sequence, and phylogenetic algorithms
3. Viral gene detection in oral neoplasms using the polymerase chain reaction
4. Viral latency-the papillomavirus model
5. Simultaneous detection and typing strategy for human papillomaviruses based on PCR and restriction endonuclease mapping
6. Type-specific human papillomavirus DNA in abnormal smears as a predictor of high-grade cervical intraepithelial neoplasia
7. Detection of HPV-16 genome in oral cancers and potentially malignant lesions from India
8. Two newly identified human papillomavirus types (HPV40 and 57) I solated from mucosal lesions
9. Improved amplification of genital human papillomaviruses
10. Degenerate and nested PCR: A highly sensitive and specific method for detection of human papillomavirus infection in cutaneous warts
11. Use of multiple primer sets for optimal detection of human papillomavirus
12. Southern blot hybridization and PCR in detection of oral human papillomavirus (HPV) infections in women with genital HPV infections
13. Human papillomavirus in normal oral mucosa: A comparison of methods for sample collection
14. Two novel genital papillomavirus (HPV) Types, HPV 68 and HPV 70, related to the potentially oncogenic HPV 39
15. A novel genital Human papillomavirus (HPV), HPV Type 74, found in immunosuppressed patients
16. Typing of human papillomaviruses by polymerase chain reaction amplification with L1 consensus primers and RFLP analysis
17. Use of polymerase chain reaction amplification for the detection of genital human papillomavirus
18. Oral antibodies to human papillomavirus type 16 in women with cervical neoplasia
19. The role of viral phenotype and CCR-5 gene defects in HIV-1 transmission and disease progression
20. Human papillomavirus and cancer: The epidemiological evidence
21. Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa
22. Simultaneous polymerase chain reaction detection and restriction typing for the diagnosis of human genital papillomavirus infection
23. Oral cancer risk in relation to sexual history and evidence of human papillomavirus infection
24. Detection of human papillomavirus DNA by in situ DNA hybridization and polymerase chain reaction in premalignant and malignant oral lesions
25. Prevalence of mucosotropic human papillomaviruses in squamous-cell carcinomas of the head and neck
26. The high risk human papillomaviruses and oral cancer: Evidence for and against a causal relationship
27. High prevalence of human papillomaviruses in the normal oral cavity of adults
28. Novel HPV types present in oral papillomatous lesions from patients with HIV infection
29. Human papillomavirus DNA types in squamous cell carcinomas of the head and neck
30. Detection of genital human papillomaviruses by polymerase chain reaction amplification with degenerate nested primers
31. Analysis of human papillomavirus DNA in oral squamous cell carcinomas