Microdensitometric assay of enzymatic activities in parthenogenetically activated and in vitro fertilized bovine oocytes

Acta Histochemica

Volumn 104, Issue 2, 2002, Pages 193-198

  Ferrandi, Bruno ^a,b   Cremonesi, Fausto ^a   Consiglio, Anna Lange ^a   Luciano, Alberto Maria ^a   Gandolfi, Fulvio ^a   Modina, Silvia ^a   Carnevali, Antino ^a   Porcelli, Franca ^a  

^a UNIVERSITY OF MILAN   (Italy)

^b Facolta di Medicina Veterinaria   (Italy)

Author keywords

Bovine; Microspectrophotometric determination; Oocyte metabolism; Parthenogenetic activation

Indexed keywords

BIOASSAY; CALCIUM; DEHYDROGENATION; GENES; METABOLISM;

FERTILIZATION;

ENZYME KINETICS;

6 N,N DIMETHYLADENINE; CALCIUM IONOPHORE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; LACTATE DEHYDROGENASE; SUCCINATE DEHYDROGENASE; ADENINE; COLORING AGENT; DRUG DERIVATIVE; IONOPHORE; N(6),N(6) DIMETHYLADENINE; N(6),N(6)-DIMETHYLADENINE;

ANIMAL CELL; ARTICLE; CATTLE; CONTROLLED STUDY; ENZYME ACTIVITY; FEMALE; FERTILIZATION IN VITRO; MICRODENSITOMETRY; MICROSPECTROPHOTOMETRY; NONHUMAN; OOCYTE; OOCYTE MATURATION; PARTHENOGENESIS; ANIMAL; DENSITOMETRY; ENZYMOLOGY; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MALE; METABOLISM; PHYSIOLOGY; PREGNANCY; ULTRASTRUCTURE;

ANIMALIA; BOS TAURUS; BOVINAE;

ADENINE; ANIMALS; CATTLE; COLORING AGENTS; DENSITOMETRY; FEMALE; FERTILIZATION IN VITRO; GLUCOSEPHOSPHATE DEHYDROGENASE; IMMUNOHISTOCHEMISTRY; IONOPHORES; L-LACTATE DEHYDROGENASE; MALE; OOCYTES; PARTHENOGENESIS; PREGNANCY; SUCCINATE DEHYDROGENASE;

EID: 0035987916     PISSN: 00651281     EISSN: None     Source Type: Journal    
DOI: 10.1078/0065-1281-00640     Document Type: Article

References (27)

1. Generation of reactive oxygen species, lipid peroxidation, and human sperm function
2. Hexokinase activity in mouse embryos developed in vivo and in vitro
3. Estimation and manipulation of glutathione levels in prepuberal mouse ovaries and ova: Relevance to sperm nucleus transformation in the fertilized egg
4. Histone HI kinase activity in bovine oocytes following calcium stimulation
5. A cytochemical method for measuring enzyme activity in individual preovulatory mouse oocytes
6. A stable propidium iodide staining procedure for flow cytometry
7. Quantitative cytochemical study of some enzymatic activities in preovulatory bovine oocytes after in vitro maturation
8. Effect of 6-dimethylaminopurine on germinal vesicle breakdown of bovine oocytes
9. Comparative analysis of calf and cow oocytes during in vitro maturation
10. The effect of in vivo glutathione depletion with buthionine sulfoximine on rat embryo development
11. Development of parthenogenetic and cloned ovine embryos: Effect of activation protocols
12. Bovine blastocyst production in vitro after inhibition of oocyte meiotic resumption for 24 h
13. Synthesis of LDH-1 during mamalian oogenesis and early development
14. Oxidative stress during the interaction of gametes
15. Capacitation of bovine sperm by heparin
16. Changes in the metabolism of glucose, pyruvate, glutamine and glycine during maturation of cattle oocytes in vitro
17. Developmental capacity of bovine oocytes following inhibition of meiotic resumption by cyclohexamide or 6-dimethylaminopurine
18. Development of glucose utilization studied in single oocytes and preimplantation embryos from mice
19. The proliferative and meiotic history of mammalian female germ cells
20. The production of antibodies against mammalian LDH-1
21. Immunochemical studies of lactate dehydrogenase and glucose-6-phosphate dehydrogenase in preimplantation mouse embryos
22. Inhibition of protein kinases after an induced calcium transient that causes transition of bovine oocytes to embryonic cycles without meiotic completion
23. Paternal contribution to the mammalian zygote: Fertilization after sperm-egg fusion
24. Quantitative aspects of the cytochemical demonstration of glucose-6-phosphate dehydrogenase with tetranitro BT studied in a model system of polyacrylamide films
25. Model film studies in enzyme histochemistry with special reference to glucose-6-phosphate dehydrogenase
26. Enzyme cytochemical staining of individual cells with the use of a polyacrylamide carrier. Studies on the synthetizing reaction technique, the indigogenic method, the metal salt method, the post-azo-coupling technique, and the tetrazolium salt technique
27. Cytophotometry of glucose-6-phosphate dehydrogenase activity in individual cells