Inhibition of bacterial pyridoxal-depending enzymes by (aminooxy)-acetic acid improves selective 15N isotope labeling of bacterially expressed protein

BioTechniques

Volumn 32, Issue 6, 2002, Pages 1248-1250

  Lopukhov, Leonid V ^a   Ponomareva, Anna A ^a   Yagodina, Lilia O ^a  

^a KAZAN INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOOXYACETIC ACID; BACTERIAL ENZYME; BACTERIAL PROTEIN; NITROGEN 15; PYRIDOXAL; AMINOTRANSFERASE; BARSTAR PROTEIN, BACILLUS AMYLOLIQUEFACIENS; ENZYME INHIBITOR; NITROGEN;

ANALYTIC METHOD; ARTICLE; CONCENTRATION RESPONSE; COST BENEFIT ANALYSIS; ENZYME INHIBITION; ESCHERICHIA COLI; ISOTOPE LABELING; MOLECULAR STABILITY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; SPECTROSCOPY; DRUG ANTAGONISM; ENZYMOLOGY; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

AMINOOXYACETIC ACID; BACTERIAL PROTEINS; CLONING, MOLECULAR; ENZYME INHIBITORS; ESCHERICHIA COLI; MAGNETIC RESONANCE SPECTROSCOPY; NITROGEN ISOTOPES; PYRIDOXAL; TRANSAMINASES;

EID: 0035987136     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02326bm06     Document Type: Article

References (9)

1. Superslow backbone protein dynamics as studied by 1D solid-state MAS exchange NMR spectroscopy
2. A novel method for selective isotope labeling of bacterially expressed proteins
3. Solution structure of barstar
4. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
5. 15N-amino acids
6. Structure determination of biological macromolecules in solution using nuclear magnetic resonance spectroscopy
7. NMR spectroscopy of large molecules and multimolecular assemblies in solution