Pancreatic β-cell protein granuphilin binds Rab3 and Munc-18 and controls exocytosis

Molecular Biology of the Cell

Volumn 13, Issue 6, 2002, Pages 1906-1915

  Coppola, Thierry ^a   Frantz, Christian ^a   Perret Menoud, Véronique ^a   Gattesco, Sonia ^a   Hirling, Harald ^a   Regazzi, Romano ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; COMPLEMENTARY DNA; GRANUPHILIN; GUANOSINE TRIPHOSPHATASE; INSULIN; MUTANT PROTEIN; N ETHYLMALEIMIDE; POLYCLONAL ANTIBODY; RAB PROTEIN; SNARE PROTEIN; SYNAPTOTAGMIN; UNCLASSIFIED DRUG; ANTIBODY; CARRIER PROTEIN; MUNC18 PROTEIN; NERVE PROTEIN; PRIMER DNA; RECOMBINANT PROTEIN; SYTL4 PROTEIN, RAT; VESICULAR TRANSPORT PROTEIN; ZINC FINGER PROTEIN;

ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; EXOCYTOSIS; INSULIN RELEASE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PANCREAS ISLET BETA CELL; PANCREAS ISLET CELL; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RAT; SECRETORY VESICLE; ANIMAL; BINDING SITE; CELL FRACTIONATION; CHEMISTRY; IMMUNOLOGY; METABOLISM; PANCREAS ISLET; PHYSIOLOGY; PLASMID; PROTEIN CONFORMATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ULTRASTRUCTURE;

ANIMAL; ANTIBODIES; BASE SEQUENCE; BINDING SITES; CARRIER PROTEINS; CLONING, MOLECULAR; DNA PRIMERS; EXOCYTOSIS; ISLETS OF LANGERHANS; NERVE TISSUE PROTEINS; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; RAB3 GTP-BINDING PROTEINS; RATS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SUBCELLULAR FRACTIONS; SUPPORT, NON-U.S. GOV'T; VESICULAR TRANSPORT PROTEINS; ZINC FINGERS; ANIMALS; MUNC18 PROTEINS;

EID: 0035985165     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.02-02-0025     Document Type: Article

References (38)

1. Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines
2. Mutants of Rab3A analogous to oncogenic Ras mutants
3. Evidence that the Rab3a-binding protein, rabphilin3a, enhances regulated secretion
4. Rabphilin dissociated from Rab3 promotes endocytosis through interaction with rabaptin-5
5. Disruption of Rab3-calmodulin interaction, but not other effector interactions, prevents Rab3 inhibition of exocytosis
6. A conformational switch in syntaxin during exocytosis: Role of munc-18
7. β-Granule transport and exocytosis
8. Synaptotagmin-like protein 1-3: A novel family of C-terminal-type tandem C2 proteins
9. Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: Identification of the Slp homology domain
10. The small GTP-binding protein Rab3A regulates a late step in synaptic vesicle fusion
11. Rab3a controls exocytosis in cholecystokinin-secreting cells
12. Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase
13. Protein modeling for all
14. 2+-regulated exocytosis in neuroendocrine cells
15. 2+-dependent exocytosis from adrenal chromaffin cells
16. Subcellular distribution and function of Rab3A, B, C, and D isoforms in insulin-secreting cells
17. The Rab3-interacting molecule RIM is expressed in pancreatic β-cells and is implicated in insulin exocytosis
18. Membrane fusion and exocytosis
19. High affinity Rab3 binding is dispensable for rabphilin-dependent potentiation of stimulated secretion
20. The GTPase Rab3a negatively controls calcium-dependent exocytosis in neuroendocrine cells
21. Molecular mechanisms and regulation of insulin exocytosis as a paradigm of endocrine secretion
22. t-SNARE activation through transient interaction with a Rab-like guanosine triphosphatase
23. Rab3A and Rab3D control the total granule number and the fraction of granules docked at the plasma membrane in PC12 cells
24. Role of the Rab3A-binding domain in targeting of rabphilin-3a to vesicle membranes of PC12 cells
25. Expression of SNARE proteins in enteroendocrine cell lines and functional role of tetanus toxin-sensitive proteins in cholecystokinin release
26. Structural basis of Rab effector specificity: Crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A
27. Evolution of the Rab family of small GTP-binding proteins
28. 2+, diacylglycerol, and protein kinase C activity
29. Expression, localization and functional role of small GTPases of the Rab3 family in insulin-secreting cells
30. Involvement of the ras-like GTPase rab3d in RBL-2H3 mast cell exocytosis following stimulation via high affinity IgE receptors (FcRI)
31. Mechanism of intracellular protein transport
32. Rabphilin-3A, a putative target protein for smg p25A/rab3A p25 small GTP-binding protein related to synaptotagmin
33. A possible target protein for smg-25/rab3A small GTP-binding protein
34. Rab3a binding and secretion-enhancing domains in Rim1 are separate and unique
35. Munc18-1 promotes large dense-core vesicle docking
36. Novel rabphilin-3-like protein associates with insulin-containing granules in pancreatic beta cells
37. Rim1 and rabphilin-3 bind Rab3-GTP by composite determinants partially related through N-terminal α-helix motifs
38. Rim is a putative Rab3 effector in regulating synaptic-vesicle fusion