Adaptable doxycycline-regulated gene expression systems for Drosophila

Gene

Volumn 270, Issue 1-2, 2001, Pages 103-111

  Stebbins, Michael J ^a,b   Yin, Jerry C P ^a  

^a Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

^b STONY BROOK UNIVERSITY   (United States)

Author keywords

Inducible; Spatial temporal; Tet Off transactivator; Tetracycline; Transgenic

Indexed keywords

ACTIN; DOXYCYCLINE; LUCIFERASE; TRANSACTIVATOR PROTEIN;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA VECTOR; DROSOPHILA; EMBRYO; ENZYME INDUCTION; FEEDING; FEMALE; GENE CONTROL; GENE EXPRESSION; GENE INDUCTION; GENE TARGETING; GENETIC ENGINEERING; LARVA; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE; TISSUE DISTRIBUTION; TRANSGENE;

ACTINS; ANIMALS; ANIMALS, GENETICALLY MODIFIED; DOSE-RESPONSE RELATIONSHIP, DRUG; DOXYCYCLINE; DROSOPHILA; EMBRYO, NONMAMMALIAN; GENE DOSAGE; GENE EXPRESSION REGULATION; GENE EXPRESSION REGULATION, DEVELOPMENTAL; KINETICS; LARVA; LUCIFERASES; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATORS; TRANSGENES;

EID: 0035972777     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(01)00447-4     Document Type: Article

References (28)

1. Tet repressor-based system for regulated gene expression in eukaryotic cells: Principles and advances
2. Spatial and temporal targeting of gene expression in Drosophila by means of a tetracycline-dependent transactivator system
3. The Drosophila dCREB2 gene affects the circadian clock
4. Doxycycline-induced transgene expression during Drosophila development and aging
5. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
6. A 5′ element of the chicken beta-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila
7. Nalyot, a mutation of the Drosophila myb-related Adf1 transcription factor, disrupts synapse formation and olfactory memory
8. Chromatin structure and gene expression
9. Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter
10. In vivo analysis of scaffold-associated regions in Drosophila: A synthetic high-affinity SAR binding protein suppresses position effect variegation
11. Tight control of gene expression in mammalian cells by tetracycline- responsive promoters
12. Transcriptional activation by tetracyclines in mammalian cells
13. A repressible female-specific lethal genetic system for making transgenic insect strains suitable for a sterile-release program
14. A position-effect assay for boundaries of higher order chromosomal domains
15. A group of scs elements function as domain boundaries in an enhancer- blocking assay
16. Dosage-sensitive and complementary functions of roundabout and commissureless control axon crossing of the CNS midline
17. The heat-shock response
18. A DNA segment controlling metal-regulated expression of the Drosophila melanogaster metallothionein gene Mtn
19. Expression of engrailed proteins in arthropods, annelids, and chordates
20. The Drosophila hsp70 message is rapidly degraded at normal temperatures and stabilized by heat shock
21. Regulation of HSP70 synthesis by messenger RNA degradation
22. A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: In situ hybridization using digoxigenin-labelled cRNA probes
23. Olfactory learning deficits in mutants for leonardo, a Drosophila gene encoding a 14-3-3 protein
24. Insect population control using a dominant, repressible, lethal genetic system
25. Genetic dissection of consolidated memory in Drosophila
26. Exploring the sequence space for tetracycline-dependent transcriptional activators: Novel mutations yield expanded range and sensitivity
27. Induction of a dominant negative CREB transgene specifically blocks long-term memory in Drosophila