Synchronization of porcine fetal fibroblast cells with topoisomerase-inhibitor hoechst 33342

Animal Reproduction Science

Volumn 66, Issue 1-2, 2001, Pages 109-116

  Kühholzer, Birgit ^a   Prather, Randall S ^a  

^a UNIVERSITY OF MISSOURI   (United States)

Author keywords

Bisbenzimide; Cell cycle; Fetal fibroblasts; Flow cytometry; G2 M synchronization

Indexed keywords

BENZIMIDAZOLE DERIVATIVE; DNA TOPOISOMERASE; ENZYME INHIBITOR; HOE 33342;

ANIMAL; ARTICLE; CELL CYCLE; CELL CYCLE G2 PHASE; CELL NUCLEUS TRANSPLANTATION; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; FETUS; FIBROBLAST; GESTATIONAL AGE; MITOSIS; PRENATAL DEVELOPMENT; SWINE;

ANIMALS; BENZIMIDAZOLES; CELL CYCLE; DNA TOPOISOMERASES, TYPE I; ENZYME INHIBITORS; FETUS; FIBROBLASTS; G2 PHASE; GESTATIONAL AGE; MITOSIS; NUCLEAR TRANSFER TECHNIQUES; SWINE;

SUIDAE;

EID: 0035971740     PISSN: 03784320     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4320(01)00088-4     Document Type: Article

References (19)

1. Catalytic inhibitors of DNA Topoisomerase II
2. Effects of analogs of the DNA minor groove binder Hoechst 3358 on Topoisomerase II and I mediated activities
3. Flow cytometric cell cycle analysis of cultured porcine fetal fibroblast cells used for nuclear transfer
4. A new mammalian DNA Topoisomerase I poison Hoechst 33342: Cytotoxicity and drug resistance in human cell cultures
5. DNA minor groove-binding ligands: A different class of DNA Topoisomerase I inhibitors
6. Reversible synchronization of cultured rodent and human diploid fibroblast cells in G2 phase
7. Dynamic imaging of the metaphase II spindle and maternal chromosomes in bovine oocytes: Implications for enucleation efficiency verification, avoidance of parthenogenesis, and successful embryogenesis
8. Cell cycle synchronization of porcine fetal fibroblasts: Effect of serum deprivation and reversible cell cycle inhibitors
9. Cell line-specific differences in the control of cell cycle progression in the absence of mitosis
10. Production of sextruplet mice by transferring metaphase nuclei from four-cell embryos
11. Porcine nuclear transfer embryos derived from injection of G2/M stage fibroblasts into preactivated in vitro matured oocytes
12. Feasibility of production of nuclear transfer pigs by using G2/M stage fibroblasts as donors
13. Effect of minor groove binding drugs on mammalian Topoisomerase I activity
14. Investigating the biological functions of DNA topoisomerases in eukaryotic cells
15. Optimization of porcine oocyte activation following nuclear transfer
16. Cell cycle synchronization: Reversible induction of G2 synchrony in cultured rodent and human diploid fibroblasts
17. Etoposide targets Topoisomerase IIα and IIβ in leukemic cells: Isoform-specific cleavable complex visualized and quantified in situ by a novel immunofluorescence technique
18. Modulation of Topoisomerase II catalytic activity by DNA minor groove binding agents distamycin, Hoechst 33258, and 4′,6-aiamine-2-phenylindole