In situ localisation of β-glucans in the cell wall of Schizosaccharomyces pombe

Yeast

Volumn 18, Issue 5, 2001, Pages 433-444

  Humbel, Bruno M ^a,b   Konomi, Mami ^a   Takagi, Tomoko ^a   Kamasawa, Naomi ^a   Ishijima, Sanae A ^a   Osumi, Masako ^a  

^a JAPAN WOMEN'S UNIVERSITY   (Japan)

^b UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Glucan; Cell division; Cell wall; Electron microscopy; Freeze substitution; High pressure freezing; Immunogold labelling; Schizosaccharomyces pombe

Indexed keywords

1,3 ALPHA GLUCAN; ALPHA GALACTOMANNAN; BETA 1,3 GLUCAN; BETA 1,6 GLUCAN; BETA GLUCAN; GALACTOMANNAN; GOLD; POLYSACCHARIDE; UNCLASSIFIED DRUG; GLUCAN;

ARTICLE; CELL DIVISION; CELL LABELING; CELL MEMBRANE; CELL SURFACE; CELL VACUOLE; CELL WALL; CHEMICAL COMPOSITION; CONTROLLED STUDY; ELECTRON; ENDOPLASMIC RETICULUM; GOLGI COMPLEX; HYPOTHESIS; IMMUNOELECTRON MICROSCOPY; MACROMOLECULE; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; SCANNING ELECTRON MICROSCOPY; SCHIZOSACCHAROMYCES POMBE; TRANSMISSION ELECTRON MICROSCOPY; METABOLISM; METHODOLOGY; SCHIZOSACCHAROMYCES; ULTRASTRUCTURE;

SCHIZOSACCHAROMYCES POMBE;

CELL WALL; GLUCANS; GOLGI APPARATUS; MICROSCOPY, IMMUNOELECTRON; SCHIZOSACCHAROMYCES;

EID: 0035970279     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.694     Document Type: Article

References (46)

1. Preparation and antigen specificity of an anti-(1→3)-β-d-glucan antibody
2. Transmission and scanning electron microscopic examination of intracellular organelles in freeze-substituted Kloeckera and Saccharomyces cerevisiae yeast cells
3. Synthesis of the yeast cell wall and its regulation
4. Histochemical demonstration of heavy metals. A revised version of the silver method suitable for both light and electron microscopy
5. Is there a role for GPIs in yeast cell-wall assembly?
6. The use of primuline to identify the septum polysaccharide of the fission yeast Schizosaccharomyces pombe
7. Freeze-dried human leukocytes stabilized with uranyl acetate during low temperature embedding or with OsO4 vapor after embedding
8. Vegetative ultrastructure
9. Identification of a putative alpha-glucan synthase essential for cell wall construction and morphogenesis in fission yeast
10. High-pressure freezing of cell suspensions in cellulose capillary tubes
11. Cell wall architecture of the fission yeast Schizosaccharomyces pombe
12. Improved structural preservation by combining freeze substitution and low temperature embedding
13. Freeze-substitution for immuno-chemistry
14. Three-dimensional analysis of protein aggregate body in Saccharomyces cerevisiae cells
15. Structure of the cell surface of the yeast Candida tropicalis and its relation to hydrocarbon transport
16. Fission yeast alpha-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function
17. Analysis of the process of glucan network formation in fission yeast using a cell free system
18. Ultrastructure of the cell wall of Schizosaccharomyces pombe following treatment with various glucanases
19. The molecular structures of some glucans from the cell walls of Schizosaccharomyces pombe
20. The location of (1→3)-β-glucans in the walls of pollen tubes of Nicotina alata using a (1→3)-β-glucan-specific monoclonal antibody
21. Glucomannoproteins in the cell wall of Saccharomyces cerevisiae contain a novel type of carbohydrate side chain
22. Localization of synthesis of beta 1,6-glucan in Saccharomyces cerevisiae
23. Recent progress in high pressure-freezing
24. The integrating power of cryofixation-based electron microscopy in biology
25. Improved structural preservation by freeze substitution
26. Cryofixation of thick specimens by high pressure freezing
27. Structural differences between two types of basidiomycete septal pore caps
28. Contrast enhancement by ruthenium tetroxide for observation of the ultrastructure of yeast cells
29. Modern acrylics for post-embedding immunostaining techniques
30. Biogenesis of yeast wall and surface components
31. The ultrastructure of yeast: Cell wall structure and formation
32. Electron microscopical study on the biosynthesis and assembly of yeast cell wall components
33. Morphological aspects of cell wall formation and cytoskeleton in yeast
34. Dynamics of cell wall formation in yeast
35. Dynamics of cell wall formation in fission yeast, Schizosaccharomyces pombe
36. Cell wall formation in regenerating protoblasts of Schizosaccharomyces pombe
37. Ultrahigh-resolution low-voltage SEM reveals ultra-structure of the glucan network formation from fission yeast protoplast
38. Freeze-substitution in virus research: A preview
39. β-1,6-Glucan synthesis in Saccharomyces cerevisiae
40. Cell wall 1,6-beta-glucan synthesis in Saccharomyces cerevisiae depends on ER glucosidases I and II, and the molecular chaperone BiP/Kar2p
41. Freeze-substitution and freeze-drying
42. Use of TEM, SEM, and STEM in imaging 1-nm colloidal gold particles
43. Electron microscopy after rapid freezing on a metal surface and substitution fixation
44. A simplified lead citrate stain for use in electron microscopy
45. Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze-substitution
46. Novel entry pathway of bovine herpesvirus 1 and 5